Construct: ORF ccsbBroad304_14203
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000676.1_s304c1, BRDN0000408083
- Derived from:
- ccsbBroadEn_14203
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PPP4R3A (55671)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14203
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | XR_001750421.2 | 4.1% | 1_3187del;3371_4420del | |
| 2 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | XR_943491.3 | 4.1% | 1_3226del;3410_4459del | |
| 3 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | NM_001284281.1 | 4% | 2.1% | 1_1705del;1782_1783ins106 |
| 4 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | XR_002957561.1 | 4% | 1_3265del;3449_4498del | |
| 5 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | XR_002957560.1 | 4% | 1_3335del;3519_4568del | |
| 6 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | NR_158976.1 | 4% | 1_2871del;3055_4573del | |
| 7 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | NM_001284280.1 | 3% | 1.5% | 1_2383del;2460_2461ins106 |
| 8 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | NM_001366432.1 | 2.9% | 1.5% | 1_2422del;2499_2500ins106 |
| 9 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | XM_005267842.2 | 2.9% | 1.5% | 1_2422del;2499_2500ins106 |
| 10 | mouse | 68734 | Ppp4r3a | protein phosphatase 4 regul... | XR_001780474.1 | 5.3% | (many diffs) | |
| 11 | mouse | 68734 | Ppp4r3a | protein phosphatase 4 regul... | NM_211355.2 | 2.7% | 1.5% | (many diffs) |
| 12 | mouse | 68734 | Ppp4r3a | protein phosphatase 4 regul... | XM_017315174.1 | 2.6% | 1.4% | (many diffs) |
| 13 | mouse | 68734 | Ppp4r3a | protein phosphatase 4 regul... | NM_001160214.1 | 2.6% | 1.5% | (many diffs) |
| 14 | mouse | 68734 | Ppp4r3a | protein phosphatase 4 regul... | XM_017315173.1 | 2.6% | 1.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 249
- ORF length:
- 183
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttgtgatgat gatgaagatg atgatgagga tgaagataag gaagatacgt taccattgtc 121 aaagaaagca aaatttgatt cataataatg gcaacggcct aggatcagta cctgttgaaa 181 aaaactggtt ctccacccct cccccataca aaatccacaa caaagcgcag tggtctcttg 241 tgaatgactg acaacctttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 301 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 361 tcaacctctg gatta