Construct: ORF ccsbBroad304_14207
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005114.1_s304c1, BRDN0000409148
- Derived from:
- ccsbBroadEn_14207
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEPDC1B (55789)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14207
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55789 | DEPDC1B | DEP domain containing 1B | XM_011543509.2 | 36.3% | 36.1% | 1_981del;1526C>A |
| 2 | human | 55789 | DEPDC1B | DEP domain containing 1B | NM_018369.3 | 35.2% | 35.1% | 1_1026del;1571C>A |
| 3 | human | 55789 | DEPDC1B | DEP domain containing 1B | NM_001145208.2 | 23.5% | 23.4% | 1_1026del;1239_1240ins186;1385C>A |
| 4 | mouse | 218581 | Depdc1b | DEP domain containing 1B | XM_006517614.3 | 42.6% | 46.5% | (many diffs) |
| 5 | mouse | 218581 | Depdc1b | DEP domain containing 1B | XM_006517613.3 | 36.7% | 40.1% | (many diffs) |
| 6 | mouse | 218581 | Depdc1b | DEP domain containing 1B | XM_006517612.3 | 32.2% | 35.2% | (many diffs) |
| 7 | mouse | 218581 | Depdc1b | DEP domain containing 1B | NM_178683.4 | 30.6% | 33.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 627
- ORF length:
- 561
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc acccctgtgt gatggctttg gtacccgaac actgatggtt cagacatttt 121 cccgttgcat cttgtgttcc aaggatgaag tggacttgga tgagttatta gctgctagat 181 tggtaacgtt tctgatggac aattaccagg aaattctgaa agtccctttg gccttgcaga 241 cctctataga ggagcgtgtg gctcatctac gaagagtcca gataaaatac ccaggagctg 301 atatggatat cactttatct gctccatcat tttgccgtca aattagtcca gaggaatttg 361 aatatcaaag atcatatggc tctcaggaac ctctggcagc cttgttggag gaagtcataa 421 cagatgccaa actctccaac aaagagaaaa agaagaaact gaagcagttt cagaaatcct 481 atcctgaagt ctatcaagaa cgatttccta caccagaaag tgcagcactt ctgtttcctg 541 aaaaacccaa accgaaacca cagctgctaa tgtgggcact aaagaagcct ttccaaccat 601 ttcaaagaaa tagaagtttt cgaatgtacc caactttctt gtacaaagtg gttggtaagc 661 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 721 ggcgcgttaa gtcgacaatc aacctctgga tta