Construct: ORF ccsbBroad304_14209
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013658.1_s304c1, BRDN0000403744
- Derived from:
- ccsbBroadEn_14209
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KDM3A (55818)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14209
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55818 | KDM3A | lysine demethylase 3A | NM_001146688.1 | 90.1% | 90% | (many diffs) |
| 2 | human | 55818 | KDM3A | lysine demethylase 3A | NM_018433.6 | 90.1% | 90% | (many diffs) |
| 3 | human | 55818 | KDM3A | lysine demethylase 3A | XM_024452994.1 | 90% | 89.9% | (many diffs) |
| 4 | human | 55818 | KDM3A | lysine demethylase 3A | XM_024452995.1 | 90% | 89.9% | (many diffs) |
| 5 | human | 55818 | KDM3A | lysine demethylase 3A | XM_017004494.1 | 68.6% | 68.5% | (many diffs) |
| 6 | human | 55818 | KDM3A | lysine demethylase 3A | XM_024452996.1 | 68.5% | 68.4% | (many diffs) |
| 7 | human | 55818 | KDM3A | lysine demethylase 3A | XM_006712051.4 | 40.2% | 40.1% | 0_1ins1980;1594G>A;1597_1983del |
| 8 | human | 55818 | KDM3A | lysine demethylase 3A | XM_024452997.1 | 40.1% | 40.1% | 0_1ins1980;1594G>A;1597_1989del |
| 9 | mouse | 104263 | Kdm3a | lysine (K)-specific demethy... | NM_001038695.3 | 80% | 81.8% | (many diffs) |
| 10 | mouse | 104263 | Kdm3a | lysine (K)-specific demethy... | NM_173001.3 | 80% | 81.8% | (many diffs) |
| 11 | mouse | 104263 | Kdm3a | lysine (K)-specific demethy... | XM_006505264.3 | 80% | 81.8% | (many diffs) |
| 12 | mouse | 104263 | Kdm3a | lysine (K)-specific demethy... | XM_006505265.1 | 60.7% | 62.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3642
- ORF length:
- 3576
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gctcacgctc ggagaaagtt ggccggtatt ggtggggagg aggtttctca 121 gtctgtccgc agccgacggc agcgatggca gccacgacag ctgggacgtg gagcgcgtcg 181 ccgagtggcc ctggctctcc gggaccattc gagctgtttc ccacaccgac gttaccaaga 241 aggatctgaa ggtgtgtgtg gaatttgatg gggaatcttg gaggaaaaga agatggatag 301 aagtctacag ccttctaagg agagcatttt tagtagaaca taatttggtt ttagctgaac 361 gaaagtcacc tgaaatttct gaacgaattg tacagtggcc tgcaataacg tacaaacctc 421 tgttggacaa agctggtttg ggatccataa cttctgttcg ctttctggga gatcaacaaa 481 gagtatttct ttctaaagac cttttgaagc ctatacagga tgtaaacagt cttcgacttt 541 ctcttacgga taatcagatt gtcagtaaag aatttcaagc tttgattgtg aagcatttag 601 atgaaagcca tcttttaaaa ggtgacaaaa acttagttgg ttcagaagta aaaatttata 661 gcttggaccc atctactcag tggttttcag caaccgttgt aaatggaaac ccagcatcaa 721 aaactcttca agtcaactgt gaggagattc cagcactgaa aattgttgat ccgtcactga 781 ttcatgttga agttgtacac gataaccttg tgacatgtgg taattctgca agaattggag 841 ctgtaaaacg caagtcttct gagaataatg gaaccctggt ttccaaacaa gcaaaatctt 901 gctctgaggc ctctcccagt atgtgtcctg tgcagtctgt acctacaaca gtttttaagg 961 agatactgct tggctgtact gcagcaactc cacctagtaa ggacccaaga cagcaaagta 1021 ctccccaggc tgccaactct ccacctaacc ttggagcaaa aattcctcaa ggatgtcata 1081 aacaaagttt accagaggaa atttcttcct gtctaaatac aaagtctgaa gctctgagaa 1141 caaaaccaga tgtctgcaaa gcagggttgc tctcaaagtc ctctcagatt ggaactggag 1201 acttgaaaat tctgactgag ccaaaaggca gctgtactca gcctaagaca aacactgatc 1261 aggaaaacag attggagtct gttccacaag cattgactgg ccttcctaag gagtgcttac 1321 ctacaaaggc ttcttctaag gcagaattgg aaattgccaa tcctcctgaa ctgcagaagc 1381 acctagaaca tgcaccttcc ccatcggatg tttcaaatgc accagaagtg aaagcaggtg 1441 tcaatagtga tagccctaat aactgttcag gaaaaaaggt agaaccttca gctttagctt 1501 gccgatcaca gaatttaaag gaatcttcag taaaagtaga taatgaaagc tgttgttcaa 1561 gaagcaacaa taaaatccag aatgccccat ccaggaagtc ggttttgaca gacccagcta 1621 aactcaaaaa gctgcaacag agtggcgagg ccttcgtaca ggatgattct tgtgtgaaca 1681 tcgtggcaca gttgcctaaa tgccgagagt gtcgcttgga cagtctccgc aaggataagg 1741 agcaacagaa ggactcacct gtgttttgcc gcttctttca cttcaggagg ttacaattca 1801 acaaacatgg tgtgttgcgg gtagaaggct tcttaacacc aaacaagtat gacaatgaag 1861 caattggctt gtggttacct ttaaccaaaa acgttgtggg gattgatttg gacacagcaa 1921 agtacatctt ggccaacatt ggagaccact tctgtcaaat ggtgatttct gaaaaggaag 1981 ctatgtcaac tattgagcca cacagacagg ttgcttggaa gcgagctgtc aaaggtgttc 2041 gagaaatgtg tgatgtgtgc gacaccacca tcttcaacct gcactgggtg tgtcctcggt 2101 gtgggtttgg agtatgtgtg gactgctacc ggatgaagag aaagaattgc caacagggtg 2161 ctgcttacaa gactttctct tggctaaaat gtgtgaagag tcagatacat gaaccagaga 2221 acttaatgcc cacacagatc attcctggaa aagcactcta tgatgttgga gacattgttc 2281 attctgtaag agcgaaatgg ggaataaagg caaactgccc ttgttcaaac aggcaattca 2341 aactcttttc aaagccagcc tcaaaggaag acctaaaaca gacttcttta gctggagaaa 2401 aaccgactct tggtgcagtg ctccagcaga atccctcagt gttggagcca gcagctgtgg 2461 gtggggaagc agcctccaag ccagccggca gcatgaagcc tgcctgtcca gccagcacat 2521 ctcctctaaa ctggctggcc gacctaacca gcgggaatgt caacaaggaa aacaaggaaa 2581 aacaaccaac aatgccaatt ttaaagaatg aaatcaaatg ccttccaccc ctcccacctt 2641 taagcaaatc cagcacagtc ctccatacgt ttaacagcac aattttgaca cccgtaagca 2701 acaacaattc tggtttcctc cggaatctct tgaattcttc tacaggaaag acagaaaatg 2761 gactcaagaa tacaccaaaa atccttgatg acatctttgc ctctttggtg caaaataaga 2821 cgacttctga tttatctaag aggcctcaag gactaaccat caagcccagc attctgggct 2881 ttgacactcc tcactattgg ctttgtgata atcgcttgct gtgcttgcaa gaccccaaca 2941 ataagagcaa ctggaatgtg tttagggagt gctggaaaca agggcagcca gtgatggtgt 3001 ctggagtgca tcataaattg aactctgaac tttggaaacc tgaatccttc aggaaagagt 3061 ttggtgagca ggaagtagac ctagttaatt gtaggaccaa tgaaatcatc acaggagcca 3121 cagtaggaga cttctgggat ggatttgaag atgttccaaa tcgtttgaaa aatgaaaaag 3181 aaccaatggt gttgaaactt aaggactggc caccaggaga agattttaga gatatgatgc 3241 cttccaggtt tgatgatctg atggccaaca ttccactgcc cgagtacaca aggcgagatg 3301 gcaaactgaa tttggcctct aggctgccaa actactttgt tcggccagat ctgggcccca 3361 agatgtataa tgcttatgga ttaatcactc ctgaagatcg gaaatatgga acaacaaatc 3421 ttcacttaga tgtatctgat gcagctaatg tcatggtcta tgtgggaatt cccaaaggac 3481 agtgtgagca agaagaagaa gtccttaaga ccatccaaga tggagattct gacgaactca 3541 caataaagcg atttattgaa ggaaaagaga agccaggagc actgtggcac atatatgctg 3601 caaaggacac ggagaagata agggaatttc ttaaaaagat atacccaact ttcttgtaca 3661 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 3721 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta