Transcript: Mouse NM_173001.3

Mus musculus lysine (K)-specific demethylase 3A (Kdm3a), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-11
Taxon:
Mus musculus (mouse)
Gene:
Kdm3a (104263)
Length:
4857
CDS:
221..4192

Additional Resources:

NCBI RefSeq record:
NM_173001.3
NBCI Gene record:
Kdm3a (104263)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_173001.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231229 GTGTCGGGCGTGCATCATAAA pLKO_005 3152 CDS 100% 13.200 18.480 N Kdm3a n/a
2 TRCN0000252743 TCGGCTTTCCCTTACTGATAA pLKO_005 688 CDS 100% 13.200 18.480 N Kdm3a n/a
3 TRCN0000252745 CTGCGAAGTTTCGTTGGATTT pLKO_005 4528 3UTR 100% 10.800 15.120 N Kdm3a n/a
4 TRCN0000231233 GCGCCACATCAGGTTCATAAC pLKO_005 3965 CDS 100% 10.800 8.640 N Kdm3a n/a
5 TRCN0000252746 ACTCCAGAGGATCGGAAATAT pLKO_005 3548 CDS 100% 15.000 10.500 N Kdm3a n/a
6 TRCN0000231232 CACGATCAGAGCTGGTATTTA pLKO_005 3848 CDS 100% 15.000 10.500 N Kdm3a n/a
7 TRCN0000231231 AGGAGCCCTTTGGCACATATA pLKO_005 3736 CDS 100% 13.200 9.240 N Kdm3a n/a
8 TRCN0000252747 GAAGTTCCTGAGCAAGTTATT pLKO_005 527 CDS 100% 13.200 9.240 N Kdm3a n/a
9 TRCN0000252744 TGCGGGTAGAAGGCTTCTTAA pLKO_005 1971 CDS 100% 13.200 9.240 N Kdm3a n/a
10 TRCN0000231230 ATGATCTGATGGCCAACATTC pLKO_005 3414 CDS 100% 10.800 7.560 N Kdm3a n/a
11 TRCN0000021151 GCTGGTATTTAGACCGATCAT pLKO.1 3858 CDS 100% 4.950 6.930 N KDM3A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_173001.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14209 pDONR223 100% 80% 81.8% None (many diffs) n/a
2 ccsbBroad304_14209 pLX_304 0% 80% 81.8% V5 (many diffs) n/a
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