Construct: ORF ccsbBroad304_14240
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010429.1_s304c1, BRDN0000410188
- Derived from:
- ccsbBroadEn_14240
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C14orf93 (60686)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14240
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60686 | C14orf93 | chromosome 14 open reading ... | NM_001282970.2 | 99.8% | 99.5% | 1181G>T;1494A>T |
| 2 | human | 60686 | C14orf93 | chromosome 14 open reading ... | NM_001130706.3 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 3 | human | 60686 | C14orf93 | chromosome 14 open reading ... | NM_001130708.3 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 4 | human | 60686 | C14orf93 | chromosome 14 open reading ... | NM_021944.4 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 5 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_005267971.1 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 6 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_005267972.2 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 7 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_011537059.1 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 8 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_011537060.3 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 9 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_011537062.2 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 10 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_017021570.2 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 11 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_017021572.2 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 12 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_017021573.1 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 13 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_017021574.2 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 14 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_024449685.1 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 15 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_024449687.1 | 92.4% | 92.1% | 1181G>T;1310_1429del;1614A>T |
| 16 | human | 60686 | C14orf93 | chromosome 14 open reading ... | NM_001282968.2 | 58.9% | 58.7% | (many diffs) |
| 17 | human | 60686 | C14orf93 | chromosome 14 open reading ... | NM_001282969.1 | 58.9% | 58.7% | (many diffs) |
| 18 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_006720231.1 | 58.9% | 58.7% | (many diffs) |
| 19 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_006720232.2 | 58.9% | 58.7% | (many diffs) |
| 20 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_017021575.2 | 58.9% | 58.7% | (many diffs) |
| 21 | human | 60686 | C14orf93 | chromosome 14 open reading ... | XM_017021576.1 | 58.9% | 58.7% | (many diffs) |
| 22 | mouse | 74359 | 4931414P19Rik | RIKEN cDNA 4931414P19 gene | NM_028890.2 | 77% | 79.1% | (many diffs) |
| 23 | mouse | 74359 | 4931414P19Rik | RIKEN cDNA 4931414P19 gene | XM_006519618.3 | 77% | 79.1% | (many diffs) |
| 24 | mouse | 74359 | 4931414P19Rik | RIKEN cDNA 4931414P19 gene | XM_006519619.3 | 77% | 79.1% | (many diffs) |
| 25 | mouse | 74359 | 4931414P19Rik | RIKEN cDNA 4931414P19 gene | XM_006519620.3 | 77% | 79.1% | (many diffs) |
| 26 | mouse | 74359 | 4931414P19Rik | RIKEN cDNA 4931414P19 gene | XM_011245210.2 | 77% | 79.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1560
- ORF length:
- 1494
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc cttcagtgcc accattctct tctcccctcc cagtggcagc gaggccagat 121 gctgctgctg cgcctgtaag agtgagacta atggaggcaa cacaggctcc cagggtggga 181 atcctcctcc cagcaccccc atcacagtga ctggacatgg cttggctgtt cagagctcag 241 agcagctcct gcatgttatc taccagcggg tcgataaggc agtgggtttg gctgaagctg 301 ctctgggtct tgccagggcc aacaatgagt tgttaaaacg tctccaggag gaagtgggtg 361 acctgaggca agggaaagtg tccatccctg atgaagatgg ggaaagccgg gcacatagtt 421 ccccacctga ggagcctggg cctctcaagg aaagtcccgg ggaagccttt aaggctctgt 481 ctgccgtgga agaggagtgt gacagcgtgg gcagcggcgt gcaggtggtg attgaggagc 541 tgcggcagct gggagcagcc tcagtggggc ctgggccttt gggcttccca gcaactcaga 601 gggacatgcg gctcccaggg tgcacgctgg ctgccagcga ggcggccccc ctgctcaatc 661 ctctggtgga tgattacgtg gcctctgagg gtgcagtaca gcgagttctg gtccctgctt 721 atgccaagca actctcacca gccacacaac tggcaatcca gcgggcaacc ccagagacag 781 gaccagaaaa tggaaccaag ctgccaccac cccgccctga ggacatgctc aatgccgctg 841 ctgcgctgga cagtgccttg gaagagtcag gccctgggag cactggggag ctgagacact 901 ctctagggct gaccgtttcc ccatgcagga ccagaggaag tgggcagaag aactccaggc 961 gcaagcggga tcttgtactc tctaaactgg tccacaatgt gcataaccac atcaccaatg 1021 acaagagatt caatgggtct gaaagcatca agtcctcttg gaatatttca gtagtgaagt 1081 ttcttctgga aaagctcaag caagagctgg tgaccagtcc ccacaattac actgataagg 1141 agctaaaagg agcctgtgtg gcctacttcc ttactaagag gcgtgagtac cgcaactccc 1201 tgaacccctt taaaggcctg aaggaaaaag aggagaagaa acttctaagt cgccgatatc 1261 ggctttttgc caaccgatcc agtatcatga ggcattttgg acctgaggac caacgtctgt 1321 ggaatgatgt gacagaggaa ctgatgtcag atgaagagga cagtcttaac gagccagaca 1381 gactgccttc tgctgaagcc cagctccttc caccagaact ttacaatcct aatttccaag 1441 aagaggaaga tgagggaggg gatgagaatg cacctggctc cccatctttt gaccaacccc 1501 acaaaacctg ctgtcctgac ttgaactcat tcattgaaat caaggtggaa aaggatgatt 1561 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1621 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1681 ggatta