Construct: ORF ccsbBroad304_14258
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013491.1_s304c1, BRDN0000410118
- Derived from:
- ccsbBroadEn_14258
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAPKAP1 (79109)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14258
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79109 | MAPKAP1 | MAPK associated protein 1 | NM_024117.3 | 99.9% | .8% | 3delG |
| 2 | human | 79109 | MAPKAP1 | MAPK associated protein 1 | NM_001006619.1 | 93.3% | .8% | (many diffs) |
| 3 | human | 79109 | MAPKAP1 | MAPK associated protein 1 | NM_001006617.3 | 93% | .7% | 3delG;959_1066del |
| 4 | human | 79109 | MAPKAP1 | MAPK associated protein 1 | NM_001006618.2 | 66.1% | .3% | (many diffs) |
| 5 | human | 79109 | MAPKAP1 | MAPK associated protein 1 | NM_001006620.1 | 56.3% | 1.2% | 0_1ins575;383_490del |
| 6 | human | 79109 | MAPKAP1 | MAPK associated protein 1 | NM_001006621.1 | 56.3% | 1.2% | 0_1ins575;383_490del |
| 7 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | XM_006497978.3 | 92.6% | .6% | (many diffs) |
| 8 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | XM_017317443.1 | 92.6% | .6% | (many diffs) |
| 9 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | NM_001290625.1 | 86.2% | .5% | (many diffs) |
| 10 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | NM_177345.4 | 86.2% | .5% | (many diffs) |
| 11 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | XM_006497975.2 | 86.2% | .5% | (many diffs) |
| 12 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | XM_011239082.2 | 86.2% | .5% | (many diffs) |
| 13 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | XM_011239083.2 | 86.2% | .5% | (many diffs) |
| 14 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | XM_011239084.2 | 86.2% | .5% | (many diffs) |
| 15 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | XM_017317446.1 | 77.7% | .2% | (many diffs) |
| 16 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | XM_006497980.3 | 75.5% | .6% | (many diffs) |
| 17 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | NM_001290626.1 | 51.9% | .9% | (many diffs) |
| 18 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | XM_006497982.2 | 51.9% | .9% | (many diffs) |
| 19 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | XM_006497983.2 | 51.9% | .9% | (many diffs) |
| 20 | mouse | 227743 | Mapkap1 | mitogen-activated protein k... | XM_011239085.2 | 51.9% | .9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 96
- ORF length:
- 30
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc ttcttggaca atccaactat cattctagct catattcgac agtcacatgt 121 gaccagtgat gacacgggaa tgtgtgagat ggttctcatt gatcatgatg ttgacctaga 181 gaagattcat cctccttcaa tgcctggaga cagtgggtca gaaattcagg gaagcaatgg 241 tgagactcag ggctatgtat atgcccagtc agtcgatatt acctcaagtt gggactttgg 301 tattagaaga cgctcaaaca cagctcaaag attagaacga ctccgaaaag agagacaaaa 361 ccagatcaaa tgcaaaaata ttcagtggaa agaaagaaat tctaagcaat cagcccagga 421 gttaaagtca ctgtttgaaa aaaaatctct caaagagaag cctccaattt ctgggaagca 481 gtcgatatta tctgtacgcc tagaacagtg ccctctgcag ctgaataacc cttttaacga 541 gtattccaaa tttgatggca agggtcatgt aggtacaaca gcaaccaaga agatcgatgt 601 ctacctccct ctgcactcga gccaggacag actgctgcca atgaccgtgg tgacaatggc 661 cagcgccagg gtgcaggacc tgatcgggct catctgctgg cagtatacaa gcgaaggacg 721 ggagccgaag ctcaatgaca atgtcagtgc ctactgcctg catattgctg aggatgatgg 781 ggaggtggac accgatttcc ccccgctgga ttccaatgag cccattcata agtttggctt 841 cagtactttg gccctggttg aaaagtactc atctcctggt ctgacatcca aagagtcact 901 ctttgttcga ataaatgctg ctcatggatt ctcccttatt caggtggaca acacaaaggt 961 taccatgaag gaaatcttac tgaaggcagt gaagcgaaga aaaggatccc agaaagtttc 1021 aggttcaagg gcagacgggg tttttgagga ggattcgcaa attgacatag ccacagtaca 1081 ggatatgctt agcagccacc attacaagtc attcaaagtc agcatgatcc acagactgcg 1141 attcacaacc gacgtacagc taggtatctc tggagacaaa gtagagatag accctgttac 1201 gaatcagaaa gccagcacta agttttggat taagcagaaa cccatctcaa tcgattccga 1261 cctgctctgt gcctgtgacc ttgctgaaga gaaaagcccc agtcacgcaa tatttaaact 1321 cacgtatcta agcaatcacg actataaaca cctctacttt gaatcggacg ctgctaccgt 1381 caatgaaatt gtgctcaagg ttaactacat cctggaatcg cgagctagca ctgcccgggc 1441 tgactacttt gctcaaaaac aaagaaaact gaacagacgt acgagcttca gcttccagaa 1501 ggagaagaaa tccgggcagc agtgcccaac tttcttgtac aaagtggttg gtaagcctat 1561 ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta accggtggcg 1621 cgttaagtcg acaatcaacc tctggatta