Construct: ORF ccsbBroad304_14292

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002034.1_s304c1, BRDN0000409494
Derived from:
ccsbBroadEn_14292
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VMP1 (81671)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14292
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81671 VMP1 vacuole membrane protein 1 NM_001329394.2 99.9% 99.2% 1204delG
2 human 81671 VMP1 vacuole membrane protein 1 NM_030938.5 99.9% 99.2% 1204delG
3 human 81671 VMP1 vacuole membrane protein 1 NM_001329395.2 96.8% 96.1% 974_1012del;1243delG
4 human 81671 VMP1 vacuole membrane protein 1 NM_001329396.2 90.8% 90.1% 302_303ins111;1093delG
5 human 81671 VMP1 vacuole membrane protein 1 NM_001329397.2 86.1% 85.4% 413_414ins168;1036delG
6 human 81671 VMP1 vacuole membrane protein 1 NM_001329399.2 75.7% 74.3% (many diffs)
7 human 81671 VMP1 vacuole membrane protein 1 NM_001329398.2 73.4% 72% (many diffs)
8 human 81671 VMP1 vacuole membrane protein 1 NM_001329400.2 65.8% 65.2% (many diffs)
9 human 81671 VMP1 vacuole membrane protein 1 NM_001329401.2 61.9% 60.5% (many diffs)
10 human 81671 VMP1 vacuole membrane protein 1 NM_001329402.2 52.6% 51.9% 0_1ins576;628delG
11 mouse 75909 Vmp1 vacuole membrane protein 1 NM_029478.3 90.9% 93.3% (many diffs)
12 mouse 75909 Vmp1 vacuole membrane protein 1 XM_006534403.3 90.9% 93.3% (many diffs)
13 mouse 75909 Vmp1 vacuole membrane protein 1 XM_006534402.2 87.1% 89.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1284
ORF length:
1218
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agagaatgga aaaaattgtg accagagacg tgtagcaatg aacaaggaac
121atcataatgg aaatttcaca gacccctctt cagtgaatga aaagaagagg agggagcggg
181aagaaaggca gaatattgtc ctgtggagac agccgctcat taccttgcag tatttttctc
241tggaaatcct tgtaatcttg aaggaatgga cctcaaaatt atggcatcgt caaagcattg
301tggtgtcttt tttactgctg cttgctgtgc ttatagctac gtattatgtt gaaggagtgc
361atcaacagta tgtgcaacgt atagagaaac agtttctttt gtatgcctac tggataggct
421taggaatttt gtcttctgtt gggcttggaa cagggctgca cacctttctg ctttatctgg
481gtccacatat agcctcagtt acattagctg cttatgaatg caattcagtt aattttcccg
541aaccacccta tcctgatcag attatttgtc cagatgaaga gggcactgaa ggaaccattt
601ctttgtggag tatcatctca aaagttagga ttgaagcctg catgtggggt atcggtacag
661caatcggaga gctgcctcca tatttcatgg ccagagcagc tcgcctctca ggtgctgaac
721cagatgatga agagtatcag gaatttgaag agatgctgga acatgcagag tctgcacaag
781actttgcctc ccgggccaaa ctggcagttc aaaaactagt acagaaagtt ggattttttg
841gaattttggc ctgtgcttca attccaaatc ctttatttga tctggctgga ataacgtgtg
901gacactttct ggtacctttt tggaccttct ttggtgcaac cctaattgga aaagcaataa
961taaaaatgca tatccagaaa atttttgtta taataacatt cagcaagcac atagtggagc
1021aaatggtggc tttcattggt gctgtccccg gcataggtcc atctctgcag aagccatttc
1081aggagtacct ggaggctcaa cggcagaagc ttcaccacaa aagcgaaatg ggcacaccac
1141agggagaaaa ctggttgtcc tggatgtttg aaaagttggt cgttgtcatg gtgtgttact
1201tcatcctatc tatcattaac tccatggcac aaagttatgc caaacgaatc cagcagcggt
1261tgaactcaag gagaaaacta aagacccaac tttcttgtac aaagtggttg gtaagcctat
1321ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta accggtggcg
1381cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)