Construct: ORF ccsbBroad304_14314
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002918.1_s304c1, BRDN0000400051
- Derived from:
- ccsbBroadEn_14314
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHA8 (84725)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14314
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_032639.4 | 99.9% | 98.8% | 1306delC |
| 2 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001363473.1 | 98.7% | 98.4% | (many diffs) |
| 3 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001350974.2 | 97.6% | 98.4% | 1301_1302insAAATCAACAGAAAACAC;1303_1317del |
| 4 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001350973.2 | 96.1% | 96% | (many diffs) |
| 5 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001197027.1 | 95.2% | 95.2% | (many diffs) |
| 6 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_005249897.2 | 94.9% | 94.5% | (many diffs) |
| 7 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_011515592.3 | 92.5% | 92.4% | 1301_1362del;1368delC;1383_1425del |
| 8 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_011515591.2 | 89% | 89% | (many diffs) |
| 9 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_017012744.1 | 89% | 89% | (many diffs) |
| 10 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001197026.2 | 84.2% | 84.2% | (many diffs) |
| 11 | human | 51054 | PLEKHA8P1 | pleckstrin homology domain ... | NR_037144.1 | 65.6% | (many diffs) | |
| 12 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001363474.1 | 58.1% | 57.7% | (many diffs) |
| 13 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_011515596.2 | 51.7% | 51.6% | (many diffs) |
| 14 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001350975.1 | 51.6% | 51.1% | (many diffs) |
| 15 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_017012746.1 | 45.9% | 45.8% | (many diffs) |
| 16 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_017012747.1 | 45.9% | 45.8% | (many diffs) |
| 17 | mouse | 231999 | Plekha8 | pleckstrin homology domain ... | NM_001164361.1 | 73.7% | 77.8% | (many diffs) |
| 18 | mouse | 231999 | Plekha8 | pleckstrin homology domain ... | NM_001001335.2 | 65.6% | 69.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gggggtgctg tacaagtgga ccaactatct gagcggttgg cagcctcgat 121 ggttccttct ctgtggggga atattgtcct attatgattc tcctgaagat gcctggaaag 181 gttgcaaagg gagcatacaa atggcagtct gtgaaattca agttcattct gtagataata 241 cacgcatgga cctgataatc cctggggaac agtatttcta cctgaaggcc agaagtgtgg 301 ctgaaagaca gcggtggctg gtggccctgg gatcagccaa ggcttgcctg actgacagta 361 ggacccagaa ggagaaagag tttgctgaaa acactgaaaa cttgaaaacc aaaatgtcag 421 aactaagact ctactgtgac ctccttgttc agcaagtaga taaaacaaaa gaagtgacca 481 caactggtgt gtccaattct gaggagggaa ttgatgtggg aactttgctg aaatcaacct 541 gtaatacttt tctgaagacc ttggaagaat gcatgcagat cgcaaatgca gccttcacct 601 ctgagctgct ctaccgcact ccaccaggat cacctcagct ggccatgctc aagtccagca 661 agatgaaaca tcctattata ccaattcata attcattgga aaggcaaatg gagttgagca 721 cttgtgaaaa tggatcttta aatatggaaa taaatggtga ggaagaaatc ctaatgaaaa 781 ataagaattc cttatatttg aaatctgcag agatagactg cagcatatca agtgaggaaa 841 atacagatga taatataaca gtccaaggtg aaataaggaa ggaagatgga atggaaaacc 901 tgaaaaatca tgacaataac ttgactcagt ctggatcaga ctcaagttgc tctccggaat 961 gcctctggga ggaaggcaaa gaagttatcc caactttctt tagtaccatg aacacaagct 1021 ttagtgacat tgaacttctg gaagacagtg gcattcccac agaagcattc ttggcatcat 1081 gttatgctgt ggttccagta ttagacaaac ttggccctac agtgtttgct cctgttaaga 1141 tggatcttgt tggaaatatt aagaaagtaa atcagaagta tataaccaac aaagaagagt 1201 ttaccactct ccagaagata gtgctgcacg aagtggaggc ggatgtagcc caggttagga 1261 actcagcgac tgaagccctc ttgtggctga agagaggtct caaatttttg aagggatttt 1321 tgacagaagt gaaaaatggg gagaaggata tccagacagc cctaagaaat caacagaaaa 1381 cacttgccca actttcttgt acaaagtggt tggtaagcct atccctaacc ctctcctcgg 1441 tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa 1501 cctctggatt a