Transcript: Mouse NM_001001335.2

Mus musculus pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8 (Plekha8), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Plekha8 (231999)
Length:
6628
CDS:
362..1786

Additional Resources:

NCBI RefSeq record:
NM_001001335.2
NBCI Gene record:
Plekha8 (231999)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001001335.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000105580 GCCGCCATTCAAGTAACATAA pLKO.1 2874 3UTR 100% 13.200 10.560 N Plekha8 n/a
2 TRCN0000142461 GCTGAAGAGAGGTCTCAAATT pLKO.1 1447 CDS 100% 13.200 9.240 N PLEKHA8P1 n/a
3 TRCN0000105581 CATCCCATCATACCAATTCAT pLKO.1 830 CDS 100% 5.625 3.938 N Plekha8 n/a
4 TRCN0000105582 CCACAGTGTTTGCTCCTGTTA pLKO.1 1278 CDS 100% 4.950 3.465 N Plekha8 n/a
5 TRCN0000105583 CCTGTTAAGATGGATCTTGTT pLKO.1 1292 CDS 100% 4.950 3.465 N Plekha8 n/a
6 TRCN0000146717 CCTGTTAAGATGGATCTTGTT pLKO.1 1292 CDS 100% 4.950 3.465 N PLEKHA8 n/a
7 TRCN0000105584 CCCTGGGAAGACAATGAAGAA pLKO.1 1124 CDS 100% 4.950 2.970 N Plekha8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001001335.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10505 pDONR223 100% 71% 73.4% None (many diffs) n/a
2 TRCN0000492207 GAGGTCTCTGCGTGAGTACTCCCC pLX_317 34.1% 71% 73.4% V5 (many diffs) n/a
3 ccsbBroadEn_14314 pDONR223 100% 65.6% 69.1% None (many diffs) n/a
4 ccsbBroad304_14314 pLX_304 0% 65.6% 69.1% V5 (not translated due to frame shift) (many diffs) n/a
5 TRCN0000470965 ATATCTTGGGGATCAGTCCGGGCA pLX_317 33% 65.6% 69.1% V5 (not translated due to frame shift) (many diffs) n/a
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