Construct: ORF ccsbBroad304_14330
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011196.1_s304c1, BRDN0000401565
- Derived from:
- ccsbBroadEn_14330
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SERPINB12 (89777)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14330
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89777 | SERPINB12 | serpin family B member 12 | NM_001307928.1 | 99.9% | 22.8% | 274_275insN |
| 2 | human | 89777 | SERPINB12 | serpin family B member 12 | XM_011526248.1 | 99.9% | 22.8% | 274_275insN |
| 3 | human | 89777 | SERPINB12 | serpin family B member 12 | XM_011526249.1 | 99.9% | 22.8% | 274_275insN |
| 4 | human | 89777 | SERPINB12 | serpin family B member 12 | XM_017026067.1 | 99.9% | 22.8% | 274_275insN |
| 5 | human | 89777 | SERPINB12 | serpin family B member 12 | XM_011526246.1 | 98.3% | 22.4% | 1_21del;295_296insN |
| 6 | human | 89777 | SERPINB12 | serpin family B member 12 | XM_005266778.3 | 97.1% | 22.1% | 1_36del;310_311insN |
| 7 | human | 89777 | SERPINB12 | serpin family B member 12 | NM_080474.2 | 95.2% | 20.7% | 243_244ins61 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 390
- ORF length:
- 321
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggactctctt gttacagcaa acaccaaatt ttgctttgat ctttttcaag 121 agataggcaa agatgatcgt cataaaaaca tatttttctc tcccctgagc ctctcagctg 181 cccttggtat ggtacgcttg ggtgctagaa gtgacagtgc acatcagatt gatgaggtac 241 tacacttcaa cgaattttcc cagaatgaaa gcaaagaacc tgacccttgt ctgaaaagca 301 acaaacaaaa agtgctggct gacagctctc tggaggggca ganaaaaaac gacagagcct 361 ctggatcagc aggctgggtc cttaaacaat gagagcggac tggtcagctg ctactttggg 421 cagcttctct ccaaattaga caggatcaag actgattaca cactgagtat tgccaacagg 481 ctttatggag agcaggaatt cccaatctgt caggaatact tagatggtgt gattcaattt 541 taccacacga cgattgaaag tgttgatttc caaaaaaacc ctgaaaaatc cagacaagag 601 attaacttct gggttgaatg tcaatcccaa ggtaaaatca aggaactctt cagcaaggac 661 gctattaatg ctgagactgt gctggtactg gtgaatgctg tttacttcaa ggccaaatgg 721 gaaacatact ttgaccatga aaacacggtg gatgcacctt tctgtctaaa tgcgaatgaa 781 aacaagagtg tgaagatgat gacgcaaaaa ggcctctaca gaattggctt catagaggag 841 gtgaaggcac agatcctgga aatgaggtac accaagggga agctcagcat gttcgtgctg 901 ctgccatctc actctaaaga taacctgaag ggtctggaag agcttgaaag gaaaatcacc 961 tatgaaaaaa tggtggcctg gagcagctca gaaaacatgt cagaagaatc ggtggtcctg 1021 tccttccccc ggttcaccct ggaagacagc tatgatctca attccatttt acaagacatg 1081 ggcattacgg atatctttga tgaaacgagg gctgatctta ctggaatctc tccaagtccc 1141 aatttgtact tgtcaaaaat tatccacaaa acctttgtgg aggtggatga aaacggtacc 1201 caggcagctg cagccactgg ggctgttgtc tcggaaaggt cactacgatc ttgggtggag 1261 tttaatgcca accacccttt tctctttttc attagacaca acaaaaccca aaccattctc 1321 ttttatggca gggtctgctc tcctttgcca actttcttgt acaaagtggt tggtaagcct 1381 atccctaacc ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg 1441 cgcgttaagt cgacaatcaa cctctggatt a