Construct: ORF ccsbBroad304_14360
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006100.1_s304c1, BRDN0000462263
- Derived from:
- ccsbBroadEn_14360
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GRIN3A (116443)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14360
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116443 | GRIN3A | glutamate ionotropic recept... | NM_133445.3 | 99.8% | 17.9% | (many diffs) |
| 2 | human | 116443 | GRIN3A | glutamate ionotropic recept... | XM_011518211.2 | 82.7% | 21.6% | (many diffs) |
| 3 | mouse | 242443 | Grin3a | glutamate receptor ionotrop... | NM_001033351.2 | 89.1% | 16.6% | (many diffs) |
| 4 | mouse | 242443 | Grin3a | glutamate receptor ionotrop... | XM_006537913.3 | 87.6% | 16.4% | (many diffs) |
| 5 | mouse | 242443 | Grin3a | glutamate receptor ionotrop... | NM_001276355.1 | 87.5% | 16.3% | (many diffs) |
| 6 | mouse | 242443 | Grin3a | glutamate receptor ionotrop... | XM_006537914.3 | 73.9% | 20.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 684
- ORF length:
- 615
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggagacta agtttgtggt ggctgctgag cagggtctgt ctgctgttgc 121 cgccgccctg cgcactggtg ctggccgggg tgcccagctc ctcctcgcac ccgcagccct 181 gccagatcct caagcgcatc gggcacgcgg tgagggtggg cgcggtgcac ttgcagccct 241 ggaccaccgc cccccgcgcg gccagccgcg ctccggacga cagccgagca ggagcccaga 301 gggatgagcc ggagccaggg actaggcggt ccccggcgcc ctcgccgggc gcacgctggt 361 tggggagcac cctgcatggc cgggggccgc cgggctcccg taagcccggg gagggcgcca 421 gggcggaggc cctgtggcca cgggacgccc tcctatttgc cgtggacaac ctgaaccgcg 481 tggaagggct gctaccctac aacctgtctt tggaagtagt gatggccatc gaggcaggcc 541 tgggcgatct gccacttttg cccttctcct cccctagttc gccatggagc agtgaccctt 601 tctccttcct gcaaagtgtg tgccataccg tggtggtgca aggggtgtcg gcgctgctcg 661 ccttccccag agccagggcg aaatgatgga gctcgacttg gtcagcttag tcctgcacat 721 tccagtgatc agcatcgtgc gccacgagtt tccacgggag agtcagaatc cccttcacct 781 acaactgagt ttagaaaatt cattaagttc tgatgctgat gtcactgtct caatcctgac 841 catgaacaac tggtacaatt ttagcttgtt gctgtgccag gaagactgga acatcaccga 901 cttcctcctc cttacccaga ataattccaa gttccacctt ggttctatca tcaacatcac 961 cgctaacctc ccctccaccc aggacctctt gagcttccta cagatccagc ttgagagtat 1021 taagaacagc acacccacag tggtgatgtt tggctgcgac atggaaagta tccggcggat 1081 tttcgaaatt acaacccagt ttggggtcat gccccctgaa cttcgttggg tgctgggaga 1141 ttcccagaat gtggaggaac tgaggacaga gggtctgccc ttagggctca ttgctcatgg 1201 aaaaacaaca cagtctgtct ttgagcacta cgtacaagat gctatggagc tggtcgcaag 1261 agctgtagcc acagccacca tgatccaacc agaacttgct ctcattccca gcacgatgaa 1321 ctgcatggag gtggaaacta caaatctcac ttcaggacaa tatttatcaa ggtttctagc 1381 caataccact ttcagaggcc tcagtggttc catcagagta aaaggttcca ccatcgtcag 1441 ctcagaaaac aactttttca tctggaatct tcaacatgac cccatgggaa agccaatgtg 1501 gacccgcttg ggcagctggc aggggggaaa gattgtcatg gactatggaa tatggccaga 1561 gcaggcccag agacacaaaa cccacttcca acatccaagt aagctacact tgagagtggt 1621 taccctgatt gagcatcctt ttgtcttcac aagggaggta gatgatgaag gcttgtgccc 1681 tgctggccaa ctctgtctag accccatgac taatgactct tccacattgg acagcctttt 1741 tagcagcctc catagcagta atgatacagt gcccattaaa ttcaagaagt gctgctatgg 1801 atattgcatt gatctgctgg aaaagatagc agaagacatg aactttgact tcgacctcta 1861 tattgtaggg gatggaaagt atggagcatg gaaaaatggg cactggactg ggctagtggg 1921 tgatctcctg agagggactg cccacatggc agtcacttcc tttagcatca atactgcacg 1981 gagccaggtg atagatttca ccagcccttt cttctccacc agcttgggca tcttagtgag 2041 gacccgagat acagcagctc ccattggagc cttcatgtgg ccactccact ggacaatgtg 2101 gctggggatt tttgtggctc tgcacatcac tgccgtcttc ctcactctgt atgaatggaa 2161 gagtccattt ggtttgactc ccaaggggcg aaatagaagt aaagtcttct ccttttcttc 2221 agccttgaac atctgttatg ccctcttgtt tggcagaaca gtggccatca aacctccaaa 2281 atgttggact ggaaggtttc taatgaacct ttgggccatt ttctgtatgt tttgcctttc 2341 cacatacacg gcaaacttgg ctgctgtcat ggtaggtgag aagatctatg aagagctttc 2401 tggaatacat gaccccaagt tacatcatcc ttcccaagga ttccgctttg gaactgtccg 2461 agaaagcagt gctgaagatt atgtgagaca aagtttccca gagatgcatg aatatatgag 2521 aaggtacaat gttccagcca cccctgatgg agtggagtat ctgaagaacg atccagagaa 2581 actagacgcc ttcatcatgg acaaagccct tctggattat gaagtgtcaa tagatgctga 2641 ctgcaaactt ctcactgtgg ggaagccatt tgccatagaa ggatacggca ttggcctccc 2701 acccaactct ccattgaccg ccaacatatc cgagctaatc agtcaataca agtcacatgg 2761 gtttatggat atgctccatg acaagtggta cagggtggtt ccctgtggca agagaagttt 2821 tgctgtcacg gagactttgc aaatgggcat caaacacttc tctgggatct ttgtgctgct 2881 gtgcattgga tttggtctgt ccattttgac caccattggt gagcacatag tatacaggct 2941 gctgctacca cgaatcaaaa acaaatccaa gctgcaatac tggctccaca ccagccagag 3001 attacacaga gcaataaata catcatttat agaggaaaag cagcagcatt tcaagaccaa 3061 acgtgtggaa aagaggtcta atgtgggacc ccgtcagctt accgtatgga atacttccaa 3121 tctgagtcat gacaaccgac ggaaatacat ctttagtgat gaggaaggac aaaaccagct 3181 gggcatccag atccaccagg acatccccct ccctccaagg agaagagagc tccctgcctt 3241 gcggaccacc aatgggaaag cagactccct aaatgtatct cggaactcag tgatgcagga 3301 actctcagag ctcgagaagc agattcaggt gatccgtcag gagctgcagc tggctgtgag 3361 caggaaaacg gagctggagg agtatcaaag gacaagtcgg acttgtgagt ccttgccaac 3421 tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac 3481 gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggatta