Construct: ORF ccsbBroad304_14406
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005572.1_s304c1, BRDN0000400628
- Derived from:
- ccsbBroadEn_14406
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEP120 (153241)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14406
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153241 | CEP120 | centrosomal protein 120 | XM_005271901.5 | 39% | 36.3% | (many diffs) |
| 2 | human | 153241 | CEP120 | centrosomal protein 120 | NM_153223.3 | 37.3% | 34.7% | (many diffs) |
| 3 | human | 153241 | CEP120 | centrosomal protein 120 | XM_024454370.1 | 37.3% | 34.7% | (many diffs) |
| 4 | human | 153241 | CEP120 | centrosomal protein 120 | NM_001166226.1 | 34.7% | 32.1% | (many diffs) |
| 5 | human | 153241 | CEP120 | centrosomal protein 120 | XM_011543185.2 | 34.7% | 32.1% | (many diffs) |
| 6 | mouse | 225523 | Cep120 | centrosomal protein 120 | XM_011246906.1 | 36% | 35.3% | (many diffs) |
| 7 | mouse | 225523 | Cep120 | centrosomal protein 120 | NM_178686.3 | 32.6% | 32.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1206
- ORF length:
- 1140
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ctccaaatcc gaccaattgc tcatcgtcgt gtccatccta gaaggtcggc 121 atttccccaa acgtccaaag catatgcttg tagtggaagc aaagtttgat ggagaacagt 181 tggctactga tcctgtggac cacactgacc agccagaatt tgctactgag ttagcttggg 241 aaattgacag gaaagcgctt catcagcaca ggctacagcg tactcctatc aaactccaat 301 gttttgcctt ggatcctgta acttcagcca aggaaaccat aggttacatc gttctggatt 361 taagaaccgc tcaagaaaca aagcaggcac caaaatggta ccagttgctg agtaataaat 421 acaccaaatt caagtctgag atacagataa gtattgcttt ggaaaccgat acaaagccac 481 cagtggatag ctttaaagca aagggggctc cccctcgaga tggaaaagta cctgccatcc 541 tggctggact tgaccccagg gacattgtgg ctgtgctgaa tgaagaggga ggctaccatc 601 agattggacc agcagaatac tgtactgact cctttattat gtcagtgacc atagcatttg 661 ctacccagtt ggaacagtta attccatgta ccatgaaact tccagaaaga cagcctgagt 721 ttttctttta ctactcttta ctgggaaatg atgttacaaa tgaacccttc aatgatttaa 781 tcaacccaaa ctttgagcca gagagagcat cagttcgcat ccgtagcagt gtagaaattc 841 ttcgtgttta cctggctctt cagtctaaac tgcagattca cctctgctgt ggagaccagt 901 cacttggaag tacagaaata cctttaactg gattacttaa aaagggcagt acagaaatca 961 accagcaccc agtcacagtc gaaggtgctt ttacccttga ccctccaaac agagccaaac 1021 agaagcttgc acctattcct gtggagctag ccccaactgt gggagtgtct gtggctctgc 1081 agagagaagg catagactcc caggatgcat tttggtattc agctctggat atcatatttc 1141 ctctgttcat cttccttttt ctggtcctag atgctataag gaaatttgca aattacgaag 1201 aaaaataccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1261 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1321 acctctggat ta