Construct: ORF ccsbBroad304_14419
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014740.1_s304c1, BRDN0000409630
- Derived from:
- ccsbBroadEn_14419
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MARCHF10 (162333)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14419
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 162333 | MARCHF10 | membrane associated ring-CH... | NM_001288780.2 | 81.8% | 81.7% | (many diffs) |
| 2 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XM_005257103.2 | 81.8% | 81.7% | (many diffs) |
| 3 | human | 162333 | MARCHF10 | membrane associated ring-CH... | NM_001100875.3 | 81.1% | 81% | (many diffs) |
| 4 | human | 162333 | MARCHF10 | membrane associated ring-CH... | NM_152598.4 | 81.1% | 81% | (many diffs) |
| 5 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XM_011524437.2 | 81% | 80.9% | (many diffs) |
| 6 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XM_005257100.3 | 79.5% | 79.3% | (many diffs) |
| 7 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XM_011524436.1 | 79.5% | 79.3% | (many diffs) |
| 8 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XM_005257102.3 | 79.3% | 79.2% | (many diffs) |
| 9 | human | 162333 | MARCHF10 | membrane associated ring-CH... | NM_001288779.2 | 77.5% | 77.4% | (many diffs) |
| 10 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XM_005257098.3 | 76.1% | 75.9% | (many diffs) |
| 11 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XM_011524435.2 | 76.1% | 75.9% | (many diffs) |
| 12 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XM_005257095.3 | 76% | 75.8% | (many diffs) |
| 13 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XM_005257096.2 | 76% | 75.8% | (many diffs) |
| 14 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XM_005257097.3 | 76% | 75.8% | (many diffs) |
| 15 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XM_005257106.4 | 68.1% | 63.1% | (many diffs) |
| 16 | human | 162333 | MARCHF10 | membrane associated ring-CH... | XR_934406.3 | 51.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2037
- ORF length:
- 1971
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaagaag 61 ttggcatgtt gcatgacgca agggtcaggc agaagttctt cagcgatgtt cagtatctgc 121 gggacatgca gcataaggtg gactctgagt atcaggcttg tctgagacga caggaatata 181 gaagagaccc aaatgagaag aaacgcgatc agttttgggg gcaagagaca agttttgaga 241 gatcccggtt ttctagcagg tcatcttcca aacagagttc tagtgaggag gatgctctaa 301 ctgaaccaag gtcatctatc aagatatctg catttaagtg tgactccaaa cttccagcaa 361 ttgaccaaac atcagtcaag cagaaacata aaagtaccat gactgtaagg aaagcagaga 421 aagtggaccc cagcgaaccc tctccagcag accaagcacc aatggtttta ttaagaaaga 481 ggaaaccaaa cctgaggaga tttacagtca gcccagaatc gcacagcccg agagcatctg 541 gggacagaag cagacagaaa cagcagtggc ctgcaaaggt gccggttccc aggggagcag 601 atcaagtagt tcaacaagaa ggcctgatgt gtaacactaa gctgaagagg ccaaatcaag 661 agagaagaaa cttggtccca tcgtcacagc caatgactga gaacgcccct gatagagcca 721 aaaaaggaga cccgagtgct ccttcccaga gtgagctgca tccagccctg tcccaggcct 781 tccaagaaaa aaatagtcct caagtattga gtgagttctc ggggccacca ctcacaccca 841 ccactgtagg agggccaaga aaggcatcat ttaggttccg agatgaagac ttttattcca 901 ttttgtcatt gaacagcaga agagaaagcg atgacactga agaggaaacc cagtctgaag 961 aatgtctgtg ggttggtgtg cgctcaccct gttctccttc ccatcacaaa agaagtagat 1021 ttggggggac atcgacccct caggccaaaa ataaaaattt tgaagaaaat gctgaaaatt 1081 gcagaggtca ttcttcaaga agaagtgagc ccagtcatgg ctcattgaga ataagcaatg 1141 caatggagcc agcaacagag cggccttctg ctgggcaaag gttgtcccaa gaccccgggc 1201 tgcctgatag ggaatctgct acagagaagg acagaggtgg cagtgaaaat gcgaaaaaga 1261 gccctctttc gtgggacacc aaatccgagc ccagacaaga ggttggtgtc aatgctgaaa 1321 atgtatggag tgattgtatt tctgtggaac ataggcctgg cacccatgat tctgaaggat 1381 actggaaaga ctatttaaac agttctcaaa attctcttga ctactttatt tctggcagac 1441 caatatctcc aagatcatca gtgaattcat cctataaccc tcctgcatca ttcatgcatt 1501 ctgctctgag agatgatatt ccagtagact tgtcaatgtc atcgacttca gttcacagct 1561 cagactcaga gggaaactca gggtttcatg tttgccaacc actgtctccc attagaaata 1621 ggactccatt tgccagtgcc gaaaaccata attatttccc agtaaacagt gcacacgaat 1681 ttgctgtcag ggaagcagaa gatactactt taacaagcca gcctcagggg gctccactat 1741 atacagatct cttactaaat ccacagggca atttgtcctt agtggattct tccagctcct 1801 ctccatcaag aatgaactca gaaggccatt tgcatgtgtc tgggtctctg caagaaaata 1861 caccatttac tttctttgca gtgtctcatt tcccaaatca aaatgataat gggagcagga 1921 tggcagcctc tggtttcaca gatgaaaagg aaaccagtaa gataaaggca gaccctgaga 1981 aactcaagaa attgcaagaa agtctcctgg aggaggactc cgaggaggag ggagactacc 2041 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2101 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 2161 tta