Construct: ORF ccsbBroad304_14478
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007290.1_s304c1, BRDN0000461253
- Derived from:
- ccsbBroadEn_14478
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIPM (340654)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14478
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340654 | LIPM | lipase family member M | NM_001128215.1 | 89.7% | 88.6% | (many diffs) |
| 2 | human | 340654 | LIPM | lipase family member M | XM_011539748.3 | 88.2% | 87.2% | (many diffs) |
| 3 | human | 340654 | LIPM | lipase family member M | XM_011539751.3 | 75.5% | 75.3% | 0_1ins264;474_494del;520C>A |
| 4 | human | 340654 | LIPM | lipase family member M | XM_011539752.3 | 59.6% | 59.4% | 0_1ins450;288_308del;334C>A |
| 5 | human | 340654 | LIPM | lipase family member M | XM_011539753.2 | 46% | 44.9% | (many diffs) |
| 6 | mouse | 78753 | Lipm | lipase, family member M | NM_023903.1 | 76.9% | 78.7% | (many diffs) |
| 7 | mouse | 78753 | Lipm | lipase, family member M | XM_006527466.4 | 76.9% | 78.7% | (many diffs) |
| 8 | mouse | 78753 | Lipm | lipase, family member M | XM_006527467.4 | 51.6% | 53% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1218
- ORF length:
- 1149
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccaggcct ttagaccgca aacagagtga aatcatccaa catcaaggct 121 atccctgtga ggaatatgaa gtcgcaactg aagatgggta tatcctttct gttaacagga 181 ttcctcgagg cctagtgcaa cctaagaaga caggttccag gcctgtggtg ttactgcagc 241 atggcctagt tggaggtgct agcaactgga tttccaacct gcccaacaat agcctgggct 301 tcattctggc agatgctggt tttgacgtgt ggatggggaa cagcagggga aacgcctggt 361 ctcgaaaaca caagacactc tccatagacc aagatgagtt ctgggctttc agttatgatg 421 agatggctag gtttgacctt cctgcagtga taaactttat tttgcagaaa acgggccagg 481 aaaagatcta ttatgtcggc tattcacagg gcaccaccat gggctttatt gcattttcca 541 ccatgccaga gctggctcag aaaatcaaaa tgtattttgc tttagcaccc atagccactg 601 ttaagcatgc aaaaagcccc gggaccaaat ttttgttgct gccagatatg atgatcaagg 661 gattgtttgg caaaaaagaa tttctgtatc agaccagatt tctcagacaa cttgttattt 721 acctttgtgg ccaggtgatt cttgatcaga tttgtagtaa tatcatgtta cttctgggtg 781 gattcaacac caacaatatg aacatgagcc gagcaagtgt atatgctgcc aacactcttg 841 ctggaacatc tgtgcaaaat attctacact ggagccaggc agtgaattct ggtgaactcc 901 gggcatttga ctgggggagt gagaccaaaa atctggaaaa atgcaatcag ccaactcctg 961 taaggtacag agtcagagat atgacggtcc ctacagcaat gtggacagga ggtcaggact 1021 ggctttcaaa tccagaagac gtgaaaatgc tgctctctga ggtgaccaac ctcatctacc 1081 ataagaatat tcctgaatgg gctcacgtgg atttcatctg gggtttggat gctcctcacc 1141 gtatgtacaa tgaaatcatc catctgatgc agcaggagga gaccaacctt tcccagggac 1201 ggtgtgaggc cgtattgttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 1261 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1321 agtcgacaat caacctctgg atta