Construct: ORF ccsbBroad304_14480
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000462.1_s304c1, BRDN0000408882
- Derived from:
- ccsbBroadEn_14480
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MTX3 (345778)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14480
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 345778 | MTX3 | metaxin 3 | NM_001010891.5 | 61.6% | 7.9% | (many diffs) |
| 2 | human | 345778 | MTX3 | metaxin 3 | NM_001363818.2 | 49% | 6.3% | (many diffs) |
| 3 | human | 345778 | MTX3 | metaxin 3 | XM_017009440.1 | 37.4% | 1.3% | (many diffs) |
| 4 | human | 345778 | MTX3 | metaxin 3 | NM_001167741.2 | 29.7% | .6% | (many diffs) |
| 5 | mouse | 382793 | Mtx3 | metaxin 3 | XM_006517713.2 | 63.7% | 8.8% | (many diffs) |
| 6 | mouse | 382793 | Mtx3 | metaxin 3 | XM_006517714.2 | 63.7% | 8.8% | (many diffs) |
| 7 | mouse | 382793 | Mtx3 | metaxin 3 | XM_006517715.2 | 63.7% | 8.8% | (many diffs) |
| 8 | mouse | 382793 | Mtx3 | metaxin 3 | XM_006517716.2 | 63.7% | 8.8% | (many diffs) |
| 9 | mouse | 382793 | Mtx3 | metaxin 3 | XM_006517712.2 | 54.2% | 7.5% | (many diffs) |
| 10 | mouse | 382793 | Mtx3 | metaxin 3 | XM_006517711.2 | 52.5% | 7.3% | (many diffs) |
| 11 | mouse | 382793 | Mtx3 | metaxin 3 | XM_006517710.2 | 48.9% | 6.8% | (many diffs) |
| 12 | mouse | 382793 | Mtx3 | metaxin 3 | NM_001162945.1 | 43% | 6% | (many diffs) |
| 13 | mouse | 382793 | Mtx3 | metaxin 3 | XR_382738.2 | 41.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 195
- ORF length:
- 126
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccccc ttggaactca gttgctgggg aggcggctgg ggactcccat 121 cggttcagcg agtccctggt ggtgatggct tatgccaaat tttctggtgc acccttgaaa 181 gtcaatgtga tagataacac ctggagaggt tcaagaggcg atgtaccaat tttgacaact 241 gaagacgaca tggtttctca gccagcaaaa atactaaact ttttaagaaa acagaaatat 301 aatgctgatt atgaactctc agcaaaacaa ggggcagata cattggctta tattgctctc 361 ctcgaagaga agcttctccc tgcagtgctt cacacattct gggttgagag tgacaattac 421 tttactgtga caaagccatg gtttgcttca caaattcctt ttcctttgag tttgatcctg 481 cctggaagaa tgtctnagca aatcttatca agtcgtgcag gaacccatat gttaagaaaa 541 ttgccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc 601 gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc 661 tggatta