Construct: ORF ccsbBroad304_14490
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011740.1_s304c1, BRDN0000460186
- Derived from:
- ccsbBroadEn_14490
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TPTEP1 (387590)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14490
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387590 | TPTEP1 | TPTE pseudogene 1 | NR_001591.1 | 18.3% | (many diffs) | |
| 2 | human | 7179 | TPTE | transmembrane phosphatase w... | NM_199260.3 | 16.4% | 4.3% | (many diffs) |
| 3 | human | 93492 | TPTE2 | transmembrane phosphoinosit... | NM_199254.2 | 15.7% | 3.9% | (many diffs) |
| 4 | human | 7179 | TPTE | transmembrane phosphatase w... | NM_199261.3 | 15.2% | 3.7% | (many diffs) |
| 5 | human | 7179 | TPTE | transmembrane phosphatase w... | NM_199259.3 | 15.1% | 4.1% | (many diffs) |
| 6 | human | 93492 | TPTE2 | transmembrane phosphoinosit... | NR_073485.1 | 11.1% | (many diffs) | |
| 7 | human | 220115 | TPTE2P3 | transmembrane phosphoinosit... | NR_002793.1 | 8.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 237
- ORF length:
- 168
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggattcacta ccagattcta ctgtatgctc tgacaactat gaccacaatg 121 gcagcaagat taagaaaatt gtgcattcaa ttatatcatc ctttgcattt tggactattt 181 ggagttttcc tggtcttact ggatgtcact ctcgtccttg ccgacctaat tttcactgac 241 agcaaacttt atattccttc ggagtatcgt tctatttctc tagctattgc cttatttttt 301 ctcatggatg ttcttcttcg agtatttgta gaagggaaac tgaacttgcc aactttcttg 361 tacaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa 421 tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta