Transcript: Human NR_073485.1

Homo sapiens transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 (TPTE2), transcript variant 6, non-coding RNA.

Source:
NCBI, updated 2018-05-12
Taxon:
Homo sapiens (human)
Gene:
TPTE2 (93492)
Length:
2213
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_073485.1
NBCI Gene record:
TPTE2 (93492)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_073485.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000355994 CATTAGGAAATTGTTCGATAT pLKO_005 1609 3UTR 100% 10.800 15.120 N TPTE2 n/a
2 TRCN0000378179 TTCGATTCGTGGTGATGTATG pLKO_005 1535 3UTR 100% 10.800 7.560 N TPTE2 n/a
3 TRCN0000355949 TTTGGAGAAAGGCGAACCAAT pLKO_005 1374 3UTR 100% 4.950 3.465 N TPTE2 n/a
4 TRCN0000010730 ATTGAGGAAGTTGTGCGGTTT pLKO.1 1059 3UTR 100% 4.050 2.835 N TPTE2 n/a
5 TRCN0000002694 GATTCGTGGTGATGTATGTGA pLKO.1 1538 3UTR 100% 3.000 2.100 N TPTE2 n/a
6 TRCN0000002693 CAAAGGAAGAACCGGGACTAT pLKO.1 1292 3UTR 100% 4.950 2.970 N TPTE2 n/a
7 TRCN0000355948 TTCTATAGAAATCCAATTGAG pLKO_005 1044 3UTR 100% 4.950 2.970 N TPTE2 n/a
8 TRCN0000002692 GCAAAGGAAGAACCGGGACTA pLKO.1 1291 3UTR 100% 4.050 2.430 N TPTE2 n/a
9 TRCN0000052816 CCACAGACAAACGAATTTAAA pLKO.1 336 3UTR 100% 15.000 7.500 Y TPTEP2 n/a
10 TRCN0000052815 CCTTTGGAGTATCGTTCTATT pLKO.1 636 3UTR 100% 13.200 6.600 Y TPTEP2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_073485.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07096 pDONR223 100% 67.1% None (many diffs) n/a
2 ccsbBroad304_07096 pLX_304 0% 67.1% V5 (many diffs) n/a
3 ccsbBroadEn_09359 pDONR223 100% 55.6% None (many diffs) n/a
4 ccsbBroad304_09359 pLX_304 0% 55.6% V5 (many diffs) n/a
5 TRCN0000476938 CCCCATGTCTGGCCCGTCAAGACC pLX_317 35.7% 55.6% V5 (many diffs) n/a
6 TRCN0000475551 ACGAACACCAGTACCGGGAGCTGG pLX_317 100% 11.1% V5 (many diffs) n/a
7 ccsbBroadEn_14490 pDONR223 100% 11.1% None (many diffs) n/a
8 ccsbBroad304_14490 pLX_304 0% 11.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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