Transcript: Human NM_199261.3

Homo sapiens transmembrane phosphatase with tensin homology (TPTE), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-06
Taxon:
Homo sapiens (human)
Gene:
TPTE (7179)
Length:
2765
CDS:
392..2047

Additional Resources:

NCBI RefSeq record:
NM_199261.3
NBCI Gene record:
TPTE (7179)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_199261.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000355931 GTTCGGTACTTGATAACATTA pLKO_005 1743 CDS 100% 13.200 18.480 N TPTE n/a
2 TRCN0000355868 TCGTTATGTACGTGATCTAAA pLKO_005 1666 CDS 100% 13.200 18.480 N TPTE n/a
3 TRCN0000050104 TACTTCTGGTTGCACACATCT pLKO.1 1874 CDS 100% 4.950 3.960 N TPTE n/a
4 TRCN0000355867 GAGAATTTATCCATCAGATTT pLKO_005 1963 CDS 100% 13.200 9.240 N TPTE n/a
5 TRCN0000355866 ACAGATAGAACAGGAACTATG pLKO_005 1415 CDS 100% 10.800 7.560 N TPTE n/a
6 TRCN0000050105 TAAAGGAGGCACAGATAGAAC pLKO.1 1405 CDS 100% 4.950 3.465 N TPTE n/a
7 TRCN0000050107 ACCTGGCGGGAGTCATCATTG pLKO.1 417 CDS 100% 3.600 2.520 N TPTE n/a
8 TRCN0000050106 CATTATTTATTCGATTCCTCG pLKO.1 1648 CDS 100% 2.160 1.296 N TPTE n/a
9 TRCN0000052815 CCTTTGGAGTATCGTTCTATT pLKO.1 758 CDS 100% 13.200 6.600 Y TPTEP2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_199261.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07096 pDONR223 100% 96.6% 96.5% None 115_168del;1409T>C n/a
2 ccsbBroad304_07096 pLX_304 0% 96.6% 96.5% V5 115_168del;1409T>C n/a
3 ccsbBroadEn_09359 pDONR223 100% 69.7% 63.7% None (many diffs) n/a
4 ccsbBroad304_09359 pLX_304 0% 69.7% 63.7% V5 (many diffs) n/a
5 TRCN0000476938 CCCCATGTCTGGCCCGTCAAGACC pLX_317 35.7% 69.7% 63.7% V5 (many diffs) n/a
6 TRCN0000475551 ACGAACACCAGTACCGGGAGCTGG pLX_317 100% 15.2% 12.5% V5 (many diffs) n/a
7 ccsbBroadEn_14490 pDONR223 100% 15.2% 3.7% None (many diffs) n/a
8 ccsbBroad304_14490 pLX_304 0% 15.2% 3.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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