Construct: ORF ccsbBroad304_14513
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018236.1_s304c1, BRDN0000404821
- Derived from:
- ccsbBroadEn_14513
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLJ43315 (644316)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14513
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 105379274 | LOC105379274 | uncharacterized LOC105379274 | XR_001756097.2 | 17.9% | (many diffs) | |
| 2 | human | 101927615 | LOC101927615 | uncharacterized LOC101927615 | XR_001754942.1 | 13.5% | (many diffs) | |
| 3 | human | 102723393 | LOC102723393 | uncharacterized LOC102723393 | XR_001756168.1 | 12.8% | (many diffs) | |
| 4 | human | 102723393 | LOC102723393 | uncharacterized LOC102723393 | XR_001756166.1 | 12.2% | (many diffs) | |
| 5 | human | 102723393 | LOC102723393 | uncharacterized LOC102723393 | XR_001756165.1 | 12.2% | (many diffs) | |
| 6 | human | 440 | ASNS | asparagine synthetase (glut... | NM_001178075.2 | 11.8% | 10.7% | (many diffs) |
| 7 | human | 440 | ASNS | asparagine synthetase (glut... | NM_001352496.2 | 11.4% | 10.3% | (many diffs) |
| 8 | human | 440 | ASNS | asparagine synthetase (glut... | NM_001673.5 | 11.4% | 10.3% | (many diffs) |
| 9 | human | 440 | ASNS | asparagine synthetase (glut... | NM_133436.3 | 11.4% | 10.3% | (many diffs) |
| 10 | human | 440 | ASNS | asparagine synthetase (glut... | NM_183356.3 | 11.4% | 10.3% | (many diffs) |
| 11 | human | 440 | ASNS | asparagine synthetase (glut... | NM_001178076.2 | 10.5% | 9.7% | (many diffs) |
| 12 | human | 440 | ASNS | asparagine synthetase (glut... | NM_001178077.1 | 10.5% | 9.7% | (many diffs) |
| 13 | human | 101927615 | LOC101927615 | uncharacterized LOC101927615 | XR_949123.2 | 10.5% | (many diffs) | |
| 14 | human | 102723393 | LOC102723393 | uncharacterized LOC102723393 | XR_001756167.1 | 10.3% | (many diffs) | |
| 15 | human | 101927615 | LOC101927615 | uncharacterized LOC101927615 | XR_949125.2 | 10.2% | (many diffs) | |
| 16 | human | 101927615 | LOC101927615 | uncharacterized LOC101927615 | XR_949124.2 | 10.2% | (many diffs) | |
| 17 | human | 101927615 | LOC101927615 | uncharacterized LOC101927615 | XR_246985.4 | 9.7% | (many diffs) | |
| 18 | human | 644316 | FLJ43315 | asparagine synthetase pseud... | NR_033856.1 | 8.1% | 1_1010del;1017_1020delCTCTinsNNNN;1221_2521del | |
| 19 | human | 101927088 | LOC101927088 | uncharacterized LOC101927088 | XR_246956.5 | 8.1% | (many diffs) | |
| 20 | human | 101927088 | LOC101927088 | uncharacterized LOC101927088 | XR_949092.3 | 7.5% | (many diffs) | |
| 21 | human | 442028 | LOC442028 | uncharacterized LOC442028 | NR_037597.1 | 5.7% | (many diffs) | |
| 22 | human | 107986827 | CZ1P-ASNS | CZ1P-ASNS readthrough | NR_147989.1 | 5.4% | (many diffs) | |
| 23 | human | 101927088 | LOC101927088 | uncharacterized LOC101927088 | XR_949093.3 | 5.3% | (many diffs) | |
| 24 | human | 101927088 | LOC101927088 | uncharacterized LOC101927088 | XR_949091.3 | 5.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 276
- ORF length:
- 210
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtg gnnnngttac agtggtgaaa tctacaacca taagaaggtg cagcaccgtt 121 ttgaatttga ataccagacc aaagtggatg gtgacataat ccttcatctt tatgacaaag 181 gaggaattga gcaaacaatt tgtatgttgg atagtgtgtt tacatttgtt ttactggatt 241 ctgctaacaa gaaactgttc ctgggcagag atacataccc aactttcttg tacaaagtgg 301 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 361 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta