Transcript: Human NR_147989.1

Homo sapiens CZ1P-ASNS readthrough (CZ1P-ASNS), long non-coding RNA.

Source:
NCBI, updated 2018-05-09
Taxon:
Homo sapiens (human)
Gene:
CZ1P-ASNS (107986827)
Length:
3531
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_147989.1
NBCI Gene record:
CZ1P-ASNS (107986827)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_147989.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000045873 GCTCTGTTACAATGGTGAAAT pLKO.1 1842 3UTR 100% 13.200 6.600 Y ASNS n/a
2 TRCN0000045877 GCTGTATGTTCAGAAGCTAAA pLKO.1 2095 3UTR 100% 10.800 5.400 Y ASNS n/a
3 TRCN0000290113 GCTGTATGTTCAGAAGCTAAA pLKO_005 2095 3UTR 100% 10.800 5.400 Y ASNS n/a
4 TRCN0000128294 GCTGAGTTTCTTCATCTACAA pLKO.1 176 3UTR 100% 4.950 2.475 Y CCZ1 n/a
5 TRCN0000045876 GTGAACATTATGAAGTCCTTT pLKO.1 2552 3UTR 100% 4.950 2.475 Y ASNS n/a
6 TRCN0000290034 GTGAACATTATGAAGTCCTTT pLKO_005 2552 3UTR 100% 4.950 2.475 Y ASNS n/a
7 TRCN0000045874 CGAGTGAAGAAATATCCGTAT pLKO.1 1816 3UTR 100% 4.050 2.025 Y ASNS n/a
8 TRCN0000290105 CGAGTGAAGAAATATCCGTAT pLKO_005 1816 3UTR 100% 4.050 2.025 Y ASNS n/a
9 TRCN0000045875 CGTCAAGTCTTTGAACGCCAT pLKO.1 3258 3UTR 100% 2.160 1.080 Y ASNS n/a
10 TRCN0000290103 CGTCAAGTCTTTGAACGCCAT pLKO_005 3258 3UTR 100% 2.160 1.080 Y ASNS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_147989.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00113 pDONR223 100% 47.6% None 1_1629del;2765_2838del;3387_3531del n/a
2 ccsbBroad304_00113 pLX_304 0% 47.6% V5 1_1629del;2765_2838del;3387_3531del n/a
3 TRCN0000471072 TGCTAAAATGAAGAACCGACTTTT pLX_317 32% 47.6% V5 1_1629del;2765_2838del;3387_3531del n/a
4 ccsbBroadEn_05860 pDONR223 100% 47.6% None (many diffs) n/a
5 ccsbBroad304_05860 pLX_304 0% 47.6% V5 (many diffs) n/a
6 TRCN0000474976 TCCTTTCCATGGACTAAAGCAGGT pLX_317 32% 47.6% V5 (many diffs) n/a
7 ccsbBroadEn_14513 pDONR223 100% 5.4% None (many diffs) n/a
8 ccsbBroad304_14513 pLX_304 0% 5.4% V5 (many diffs) n/a
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