Transcript: Human NM_001673.5

Homo sapiens asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
ASNS (440)
Length:
2385
CDS:
118..1803

Additional Resources:

NCBI RefSeq record:
NM_001673.5
NBCI Gene record:
ASNS (440)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001673.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000045873 GCTCTGTTACAATGGTGAAAT pLKO.1 330 CDS 100% 13.200 6.600 Y ASNS n/a
2 TRCN0000045877 GCTGTATGTTCAGAAGCTAAA pLKO.1 583 CDS 100% 10.800 5.400 Y ASNS n/a
3 TRCN0000290113 GCTGTATGTTCAGAAGCTAAA pLKO_005 583 CDS 100% 10.800 5.400 Y ASNS n/a
4 TRCN0000045876 GTGAACATTATGAAGTCCTTT pLKO.1 1040 CDS 100% 4.950 2.475 Y ASNS n/a
5 TRCN0000290034 GTGAACATTATGAAGTCCTTT pLKO_005 1040 CDS 100% 4.950 2.475 Y ASNS n/a
6 TRCN0000045874 CGAGTGAAGAAATATCCGTAT pLKO.1 304 CDS 100% 4.050 2.025 Y ASNS n/a
7 TRCN0000290105 CGAGTGAAGAAATATCCGTAT pLKO_005 304 CDS 100% 4.050 2.025 Y ASNS n/a
8 TRCN0000045875 CGTCAAGTCTTTGAACGCCAT pLKO.1 1672 CDS 100% 2.160 1.080 Y ASNS n/a
9 TRCN0000290103 CGTCAAGTCTTTGAACGCCAT pLKO_005 1672 CDS 100% 2.160 1.080 Y ASNS n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001673.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00113 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00113 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000471072 TGCTAAAATGAAGAACCGACTTTT pLX_317 32% 100% 100% V5 n/a
4 ccsbBroadEn_05860 pDONR223 100% 99.9% 99.8% None 629T>A n/a
5 ccsbBroad304_05860 pLX_304 0% 99.9% 99.8% V5 629T>A n/a
6 TRCN0000474976 TCCTTTCCATGGACTAAAGCAGGT pLX_317 32% 99.9% 99.8% V5 629T>A n/a
7 ccsbBroadEn_14513 pDONR223 100% 11.4% 10.3% None (many diffs) n/a
8 ccsbBroad304_14513 pLX_304 0% 11.4% 10.3% V5 (many diffs) n/a
Download CSV