Construct: ORF ccsbBroad304_14546
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019059.1_s304c1, BRDN0000459858
- Derived from:
- ccsbBroadEn_14546
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- AXL (558)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14546
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 558 | AXL | AXL receptor tyrosine kinase | NM_021913.5 | 92.1% | 75.9% | (many diffs) |
| 2 | human | 558 | AXL | AXL receptor tyrosine kinase | NM_001699.6 | 91.1% | 74.9% | (many diffs) |
| 3 | human | 558 | AXL | AXL receptor tyrosine kinase | NM_001278599.1 | 62.6% | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2286
- ORF length:
- 2217
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtggcgg tgccccagga tgggcagggt cccgctggcc tggtgcttgg 121 cgctgtgcgg ctgggcgtgc atggccccca ggggcacgca ggctgaagaa agtcccttcg 181 tgggcaaccc agggaatatc acaggtgccc ggggactcac gggcaccctt cggtgtcagc 241 tccaggttca gggagagccc cccgaggtac attggcttcg ggatggacag atcctggagc 301 tcgcggacag cacccagacc caggtgcccc tgggtgagga tgaacaggat gactggatag 361 tggtcagcca gctcagaatc acctccctgc agctttccga cacgggacag taccagtgtt 421 tggtgtttct gggacatcag accttcgtgt cccagcctgg ctatgttggg ctggagggct 481 tgccttactt cctggaggag cccgaagaca ggactgtggc cgccaacacc cccttcaacc 541 tgagctgcca agctcaggga cccccagagc ccgtggacct actctggctc caggatgctg 601 tccccctggc cacggctcca ggtcacggcc cccagcgcag cctgcatgtt ccagggctga 661 acaagacatc ctctttctcc tgcgaagccc ataacgccaa gggggtcacc acatcccgca 721 cagccaccat cacagtgctc ccccagcagc cccgtaacct ccacctggtc tcccgccaac 781 ccacggagct ggaggtggct tggactccag gcctgagcgg catctacccc ctgacccact 841 gcaccctgca ggctgtgctg tcagacgatg ggatgggcat ccaggcggga gaaccagacc 901 ccccagagga gcccctcacc tcgcaagcat ccgtgccccc ccatcagctt cggctaggca 961 gcctccatcc tcacacccct tatcacatcc gcgtggcatg caccagcagc cagggcccct 1021 catcctggac ccactggctt cctgtggaga cgccggaggg agtgcccctg ggcccccctg 1081 agaacattag tgctacgcgg aatgggagcc aggccttcgt gcattggcaa gagccccggg 1141 cgcccctgca gggtaccctg ttagggtacc ggctggcgta tcaaggccag gacaccccag 1201 aggtgctaat ggacataggg ctaaggcaag aggtgaccct ggagctgcag ggggacgggt 1261 ctgtgtccaa tctgacagtg tgtgtggcag cctacactgc tgctggggat ggaccctgga 1321 gcctcccagt acccctggag gcctggcgcc cagggcaagc acagccagtc caccagctgg 1381 tgaaggaacc ttcaactcct gccttctcgt ggccctggtg gtatgtactg ctaggagcag 1441 tcgtggccgc tgcctgtgtc ctcatcttgg ctctcttcct tgtccaccgg cgaaagaagg 1501 agacccgtta tggagaagtg tttgaaccaa cagtggaaag aggtgaactg gtagtcaggt 1561 accgcgtgcg caagtcctac agtcgtcgga ccactgaagc taccttgaac agcctgggca 1621 tcagtgaaga gctgaaggag aagctgcggg atgtgatggt ggaccggcac aaggtggccc 1681 tggggaagac tctgggagag ggagagtttg gagctgtgat ggaaggccag ctcaaccagg 1741 acgactccat cctcaaggtg gctgtgaaga cgatgaagat tgccatctgc acgaggtcag 1801 agctggagga tttcctgagt gaagcggtct gcatgaagga atttgaccat cccaacgtca 1861 tgaggctcat cggtgtctgt ttccagggtt ctgaacgaga gagcttccca gcacctgtgg 1921 tcatcttacc tttcatgaaa catggagacc tacacagctt cctcctctat tcccggctcg 1981 gggaccagcc agtgtacctg cccactcaga tgctagtgaa gttcatggca gacatcgcca 2041 gtggcatgga gtatctgagt accaagagat tcatacaccg ggacctGGGG GTCAGATTTT 2101 TGCCTAGCTC ACATCCCCAC ATCTCCCCTG TACCAGATGT CGCTTCGAGT TCTACAAAAA 2161 GATCGGACAT CATGTCCTAC TCCCGGCACG TGTTGTATGT GGCAAGGATA CCAGTCAATG 2221 TGGATTGGCA ATGCACAAGT ATAGCACAAC CATTTCTACT CCATCAAGAG GGACATGTGG 2281 TGCTTTAAGG AAGCCCAACG CGGCTGATTC CCACACGAGG CCAATCCAGA TATGCAGGTG 2341 ATAAAACACT GGACTTTATG CTCTTTACGC CGGGGCAATG GCCTGAAGCA GCTGGCGGAG 2401 TGTATCGATG GTCCGTATGC CCGATATCGC GGCGCTGGTA GCTAAAGCCC CAGGACAGCT 2461 CAATTAATCA AAGCTGCCAC TGCTTTGTAG AACACGGTCA GGCCTGCCCA TTTCCCAGGA 2521 GCCAGACGTA ATCCTCTTTT TTCCCCCTGC ACGAGGGCAG AGGTTACTCT GTCCCCCTCG 2581 TAGACACAAG AGGAGCTGAG CCACCAGTCC AGCCGGACCA TAAGGATACC AGTAGGTGCC 2641 TCCCTGCGGT TCAGGTCCAT CCGCATGGAG GGTATGTCCA GGACCATTCC CCAACCCCTA 2701 GCCCCGCTCA GCGGGTCAAT AGGGTCTCCG CCACAGCCCC AGGGCAGGAG GAGGATGCCC 2761 CACCAGCTTT CTTGACCAAT GTGGTTGAAA AGGCTATCCC TACCCCTTCC TCGCCCTCTC 2821 TTCTGTGTAG TTATGAGGAA TAATGACCTG GGGCTCGGAA AGGGCGCGTT CAACCAAAAT 2881 CAATCTAATT ACAAAATAAA AAAAAATAGT GTGGGGTATT TTGATCTATG TTCTCTTTGT 2941 GCTATGTGTG GATACGGCGT TAAAAAGCCT TTGAATCCAG GATTTA