Construct: ORF ccsbBroad304_14567

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010472.1_s304c1, BRDN0000406392
Derived from:
ccsbBroadEn_14567
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CAPNS1 (826)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14567
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 826 CAPNS1 calpain small subunit 1 XM_005259295.1 99.8% 99.6% 957T>A
2 human 826 CAPNS1 calpain small subunit 1 XM_005259296.1 99.8% 99.6% 957T>A
3 human 826 CAPNS1 calpain small subunit 1 NM_001003962.3 82.4% 80.4% (many diffs)
4 human 826 CAPNS1 calpain small subunit 1 NM_001302632.2 82.4% 80.4% (many diffs)
5 human 826 CAPNS1 calpain small subunit 1 NM_001749.4 82.4% 80.4% (many diffs)
6 human 826 CAPNS1 calpain small subunit 1 XM_024451732.1 62% 59.4% (many diffs)
7 human 826 CAPNS1 calpain small subunit 1 XM_011527359.1 61.1% 58.8% (many diffs)
8 human 826 CAPNS1 calpain small subunit 1 NM_001302633.2 47.7% 45.8% (many diffs)
9 mouse 12336 Capns1 calpain, small subunit 1 NM_009795.4 72.8% 76.2% (many diffs)
10 mouse 12336 Capns1 calpain, small subunit 1 XM_006539488.2 72.8% 76.2% (many diffs)
11 mouse 12336 Capns1 calpain, small subunit 1 XM_011250419.2 56.2% 55.3% (many diffs)
12 mouse 12336 Capns1 calpain, small subunit 1 XM_011250420.1 42.9% 43.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1032
ORF length:
966
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt cctggttaac tcgttcttga agggcggcgg cggcggcggc gggggaggcg
121ggggcctggg tgggggcctg ggaaatgtgc ttggaggcct gatcagcggg gccgggggcg
181gcggcggcgg cggcggcggc ggcggcggtg gtggaggcgg cggtggcggt ggaacggcca
241tgcgcatcct aggcggagtc atcagcgcca tcagcgaggc ggctgcgcag tacaacccgg
301agcccccgcc cccacgcaca cattactcca acattgaggc caacgagagt gaggaggtcc
361ggcagttccg gagactcttt gcccagctgg ctggagatga catggaggtc agcgccacag
421aactcatgaa cattctcaat aaggttgtga cacgacaccc tgatctgaag actgatggtt
481ttggcattga cacatgtcgc agcatggtgg ccgtgatgga tagcgacacc acaggcaagc
541tgggctttga ggaattcaag tacttgtgga acaacatcaa aaggtggcag gccatataca
601aacagttcga cactgaccga tcagggacca tttgcagtag tgaactccca ggtgcctttg
661aggcagcagg gttccacctg aatgagcatc tctataacat gatcatccga cgctactcag
721atgaaagtgg gaacatggat tttgacaact tcatcagctg cttggtcagg ctggacgcca
781tgttccgtgc cttcaaatct cttgacaaag atggcactgg acaaatccag gtgaacatcc
841aggaggtaag gacccccata ttggggtatg ggtgcctggg aggaccccac ccctcagccc
901ttcataccag ctctgagctg cagtcccctt cctcctattt tgccagccgt ccctgggtga
961gggcaaaggg gctggtgctc ttggggttcc ctgtcctcac tctccaccct cctctcccca
1021gaggctgcag ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1081tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1141caatcaacct ctggatta

Download FASTA (ORF) (Full)