Construct: ORF ccsbBroad304_14582
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018963.1_s304c1, BRDN0000459447
- Derived from:
- ccsbBroadEn_14582
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CHEK1 (1111)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14582
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001114121.2 | 98.6% | 45.4% | (many diffs) |
| 2 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001114122.2 | 98.6% | 45.4% | (many diffs) |
| 3 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001274.5 | 98.6% | 45.4% | (many diffs) |
| 4 | human | 1111 | CHEK1 | checkpoint kinase 1 | XM_017017146.2 | 98.6% | 45.4% | (many diffs) |
| 5 | human | 1111 | CHEK1 | checkpoint kinase 1 | XM_024448337.1 | 98.6% | 45.4% | (many diffs) |
| 6 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001244846.1 | 91.4% | 48.8% | (many diffs) |
| 7 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001330428.1 | 78.8% | 25.4% | (many diffs) |
| 8 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001330427.1 | 69.7% | 25.2% | (many diffs) |
| 9 | human | 1111 | CHEK1 | checkpoint kinase 1 | XM_011542560.2 | 69.7% | 25.2% | (many diffs) |
| 10 | human | 1111 | CHEK1 | checkpoint kinase 1 | NR_045205.1 | 38% | (many diffs) | |
| 11 | human | 1111 | CHEK1 | checkpoint kinase 1 | NR_045204.1 | 28.4% | (many diffs) | |
| 12 | mouse | 12649 | Chek1 | checkpoint kinase 1 | NM_007691.5 | 88.1% | 43.9% | (many diffs) |
| 13 | mouse | 12649 | Chek1 | checkpoint kinase 1 | XM_017313117.1 | 69% | 23.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 798
- ORF length:
- 732
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agtgcccttt gtggaagact gggacttggt gcaaaccctg ggagaaggtg 121 cctatggaga agttcaactt gctgtgaata gagtaactga agaagcagtc gcagtgaaga 181 ttgtagatat gaagcgtgcc gtagactgtc cagaaaatat taagaaagag atctgtatca 241 ataaaatgct aaatcatgaa aatgtagtaa aattctatgg tcacaggaga gaagacaata 301 tccaatattt atttctggag tactgtagtg gaggagagct ttttgacaga atagagccag 361 acataggcat gcctgaacca gatgctcaga gattcttcca tcaactcatg gcaggggtgg 421 tttatctgca tggtattgga ataactcaca gggatattaa accagaaaat cttctgttgg 481 atgaaaggga taacctcaaa atctcagact ttggcttggc aacagtattt cggtataata 541 atcgtgagcg tttgttgaac aagatgtgtg gtactttacc atatgttgct ccagaacttc 601 tgaagagaag agaatttcat gcagaaccag ttgatgtttg gtcctgtgga atagtactta 661 ctgcaatgct cgctggagaa ttgccatggg acnancccag tgaccagctg tcaggagtat 721 ttctgactgg aaagaaaaaa aaaccantnc ctcnaccctt ggaaaaaaat cgattctgct 781 cctctagctc tgctgcataa aatcttagtt gagannncat cagcannnnt taccattcca 841 gacatcaaaa aagatagatg gtacaacaaa ccccctcaag aaaggggcna aaaggccccg 901 agtcacttca ggtggtgtgt cagagtctcc cagtggattt tctaagcaca ttcaatccaa 961 tttggacttc tctccagtaa acagtgcttc tagtgaagaa aatgtgaagt actccagttc 1021 tcagccagaa ccccgcacag gtctttcctt atgggatacc agcccctcat acattgataa 1081 attggtacaa gggatcagct tttcccagcc cacatgtcct gatcatatgc ttttgaatag 1141 tcagttactt ggcaccccag gatcctcaca gaacccctgg cagcggttgg tcaaaagaat 1201 gacacgattc tttaccaaat tggatgcaga caaatcttat caatgcctga aagagacttg 1261 tgagaagttg ggctatcaat ggaagaaaag ttgtatgaat caggttacta tatcaacaac 1321 tgataggaga aacaataaac tcattttcaa agtgaatttg ttagaaatgg atgataaaat 1381 attggttgac ttccggcttt ctaagggtga tggattggag ttcaagagac acttcctgaa 1441 gattaaaggg aagctgattg atattgtgag cagccagaag gtttggcttc ctgccacatg 1501 cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga 1561 ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg 1621 gatta