Construct: ORF ccsbBroad304_14608

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018964.1_s304c1, BRDN0000459448
Derived from:
ccsbBroadEn_14608
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
DGKG (1608)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14608
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1608 DGKG diacylglycerol kinase gamma NM_001346.3 99% 27.8% (many diffs)
2 human 1608 DGKG diacylglycerol kinase gamma NM_001080744.2 95.9% 28.7% (many diffs)
3 human 1608 DGKG diacylglycerol kinase gamma NM_001080745.2 94.1% 29.2% (many diffs)
4 mouse 110197 Dgkg diacylglycerol kinase, gamma NM_138650.2 87% 22.3% (many diffs)
5 mouse 110197 Dgkg diacylglycerol kinase, gamma XM_017316850.1 82.2% 21.1% (many diffs)
6 mouse 110197 Dgkg diacylglycerol kinase, gamma NM_001346673.1 82.2% 23.5% (many diffs)
7 mouse 110197 Dgkg diacylglycerol kinase, gamma XM_017316851.1 77.6% 22.1% (many diffs)
8 mouse 110197 Dgkg diacylglycerol kinase, gamma XM_017316852.1 72.6% 23.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
738
ORF length:
672
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg tgaagaacgg tgggtctccc tcactccaga agaatttgac caactccaga
121aatattcaga atattcctcc aagaagataa aagatgcctt gactgaattt aatgagggtg
181ggagcctcaa acaatatgac ccacatgagc cgattagcta tgatgtcttc aagctgttca
241tgagggcgta cctggaggtg gaccttcccc agccactgag cactcacctc ttcctggcct
301tcagccagaa gcccagacac gagacctctg accacccgac ggagggagcc agcaacagtg
361aggccaacag cgcagatact aatatacaga atgcagataa tgccaccaaa gcagacgagg
421cctgtgcccc tgatactgaa tcaaatatgg ctgagaagca agcaccagct gaagaccaag
481tggctgcgag ccccctggaa ccccccgtcc ctcggtcttc aagctcggaa tccccagtgg
541tgtacctgaa ggatgttgtg tgctacctgt ccctgctgga gacggggagg cctcaggata
601agctggagtt catgtttcgc ctctatgatt cagatgagaa cggtctcctg gaccaagcgg
661agatggattg cattgtcaac caaatgctgc atattgccca gtacctggag tgggatccca
721cagagctgag cctatattga aggagatgct gcaagggatg gactacgacc gggacggctt
781tgtgtctcta caggaatggg tccatggagg atgacacatc ccatgctgtc ctcctgggga
841tgatgactct ggctccatgg ggatgggcgg cacgcctgga acatgaagca cttcaagaaa
901ccaacctact gcaacttctg ccatatcatg ctcatgggcg tccgcaagca aggcctgtgc
961tgcacttact gtaaatacac tgtccacgaa cgctgtgtgt ccaaaaacat tcctggttgt
1021gtcaaaacgt actcaaaagc caaaaggagt ggtgaggtga tgcagcacgc atgggtggaa
1081gggaactcct ccgtcaagtg tgaccggtgc cacaaaagta tcaagtgcta ccagagtgtc
1141accgcgcggc actgcgtgtg gtgccggatg acgtttcacc gcaaatgtga attatcaacg
1201ttgtgtgacg gtggggaact cagagaccac atcttactgc ccacctccat atgccccatc
1261acccgggaca ggccaggtga gaagtctgat ggctgcgtgt ccgccaaggg cgaacttgtc
1321atgcagtata agatcatccc caccccgggt acccaccccc tgctggtctt ggtgaacccc
1381aagagtggag ggagacaagg agaaagaatt cttcggaaat tccactatct gctcaacccc
1441aaacaagttt tcaacctgga caatgggggg cctactccag ggttgaactt tttccgtgat
1501actccagact tccgtgtttt ggcctgtggt ggagatggga cagttggctg gattttggat
1561tgcattgata aggccaactt tgcaaagcat ccaccagtgg ctgtcctgcc tcttggaaca
1621ggaaatgacc ttgcccgttg tttccgcggg gaaggaggtt atgaaggggg gcagcttgac
1681aaaaatcctg aaagacattg agcagagccc cttggtgatg ctggaccgct gcatctggna
1741agtcatcccc agagaggaag tgaaaacggg gacccaggtc ccatacagca tcatgaacaa
1801ctatttctcc attggtgtgg acgcttccat tgcacacaga ttccatgtga tgagagagaa
1861acatcctgaa aaattcaaca gcaggatgaa gaacaagctg tggtactttg aatttggcac
1921ctcggagact tttgcagcga cctgcaagaa actccacgac cacattgagt tggagtgtga
1981tggggttggg gtggacctga gcaacatctt cctggaaggc attgccattc tcaacattcc
2041cagcatgtac ggaggcacca atctctgggg agaaaacaag aagaaccggg ctgtgatccg
2101ggaaagcagg aagggtgtca ctgaccccaa agaactgaaa ttctgcgttc aagacctcag
2161tgaccagctc cttgaagtgg tggggctaga aggagccatg gagatggggc agatctacac
2221cggcctgaag agtgcaggca ggaggctggc ccagtgcgcc tctgtcacca tcaggacaaa
2281caagctgctg ccaatgcaag tggatggaga accctggatg cagccatgtt gcacgattaa
2341aattactcac aagaaccaag cgcccatgat gatggggcct ccccagaaga gcagcttctt
2401ctcgttgaga aggaagagcc gttcaaaaga cttgccaact ttcttgtaca aagtggttgg
2461taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa
2521ccggtggcgc gttaagtcga caatcaacct ctggatta

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