Construct: ORF ccsbBroad304_14620
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002556.1_s304c1, BRDN0000405422
- Derived from:
- ccsbBroadEn_14620
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DUT (1854)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14620
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1854 | DUT | deoxyuridine triphosphatase | NM_001025248.2 | 99.2% | 13.7% | (many diffs) |
| 2 | human | 1854 | DUT | deoxyuridine triphosphatase | XM_017021988.1 | 75.4% | 17.3% | (many diffs) |
| 3 | human | 1854 | DUT | deoxyuridine triphosphatase | NM_001330286.2 | 65% | 7.2% | (many diffs) |
| 4 | human | 1854 | DUT | deoxyuridine triphosphatase | NM_001948.4 | 63.9% | 2.3% | (many diffs) |
| 5 | human | 1854 | DUT | deoxyuridine triphosphatase | NM_001025249.1 | 55.6% | 3.8% | 0_1ins332;38C>N;42_43delCCinsTT |
| 6 | human | 1854 | DUT | deoxyuridine triphosphatase | XR_931760.1 | 54.4% | (many diffs) | |
| 7 | human | 1854 | DUT | deoxyuridine triphosphatase | XR_001751125.2 | 28.1% | (many diffs) | |
| 8 | human | 147658 | ZNF534 | zinc finger protein 534 | NM_001291369.3 | 18.3% | 12% | (many diffs) |
| 9 | human | 147658 | ZNF534 | zinc finger protein 534 | NM_001291368.3 | 16.2% | 9.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 255
- ORF length:
- 186
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gactcccctc tgccctcgcc ccgcgctctg ctaccatttc cttacgtctc 121 tgcttcgctc agcgatgcaa aacgcgcgag gcgagggcag aggggcgaag ccgcggtact 181 ctccgggcca ggcccgcccc tcggccgcgc cgcgcagcac gggattcccc ggccgctgtc 241 cagcgctggc cgcctgagcc aaggctgccg cggagccagt acagtcgggg ccgctggctg 301 gaagggcgag cttcctaagg cggggggaag cccggcgccg gggccggaga cacccgccat 361 ttcacccagt aagcgggccc ggcctgcgga ggtgggcggc atgcagctcc gctttgcccg 421 gctctccgag cacgccangg cttccacccg gggctccgcg cgcgccgcgg gctacgacct 481 gtacagtgcc tatgattaca caataccacc tatggagaaa gctgttgtga aaacggacat 541 tcagatagcg ctcccttctg ggtgttatgg aagagtggct ccacggtcag gcttggctgc 601 aaaacacttt attgatgtag gagctggtgt catagatgaa gattatagag gaaatgttgg 661 tgttgtactg tttaattttg gcaaagaaaa gtttgaagtc aaaaaaggtg atcgaattgc 721 acagctcatt tgcgaacgga ttttttatcc agaaatagaa gaagttcaag ccttggatga 781 caccgaaagg ggttcaggag gttttggttc cactggaaag aatttgccaa ctttcttgta 841 caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg 901 agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta