Construct: ORF ccsbBroad304_14634

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018927.1_s304c1, BRDN0000459411
Derived from:
ccsbBroadEn_14634
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
ERN1 (2081)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14634
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2081 ERN1 endoplasmic reticulum to nu... NM_001433.5 99.1% 25.2% (many diffs)
2 human 2081 ERN1 endoplasmic reticulum to nu... XM_017024347.2 95% 22.9% (many diffs)
3 human 2081 ERN1 endoplasmic reticulum to nu... XM_017024348.2 91.7% 19.6% (many diffs)
4 mouse 78943 Ern1 endoplasmic reticulum (ER) ... NM_023913.2 87.9% 24.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
825
ORF length:
756
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gccggcccgg cggctgctgc tgctgctgac gctgctgctg cccggcctcg
121ggatttttgg aagtaccagc acagtgacgc ttcctgaaac cttgttgttt gtgtcaacgc
181tggatggaag tttgcatgct gtcagcaaga ggacaggctc aatcaaatgg actttaaaag
241aagatccagt cctgcaggtc ccaacacatg tggaagagcc tgcctttctc ccagatccta
301atgatggcag cctgtatacg cttggaagca agaataatga aggcctgacg aaacttcctt
361ttaccatccc agaattggtg caggcatccc catgccgaag ttcagatgga atcctctaca
421tgggtaaaaa gcaggacatc tggtatgtta ttgacctcct gaccggagag aagcagcaga
481ctttgtcatc ggcctttgca gatagtctct gcccatcaac ctctcttctg tatcttgggc
541gaacagaata caccatcacc atgtacgaca ccaaaacccg agagctccgg tggaatgcca
601cctactttga ctatgcggcc tcactgcctg aggacgacgt ggactacaag atgtcccact
661ttgtgtccaa tggtgatggg ctggtggtga ctgtggacag tgaatctggg gacgtcctgt
721ggatccaaaa ctacgcctcc cctgtggtgg ccttttatgt ctggcagcgg gagggtctga
781ggaaggtgat gcacatcaat gtcgctgtga gaccctgcgc tatctgacct tcatgtctgg
841gggaggtggg ggcgcatcac aaagtggaag tacccgttcc ccaaagagac agaggccaag
901agcaagctga cgcccactct gtatgttggg aaatactcta ccagcctcta tgcctctccc
961tcaatggtac acgagggggt tgctgtcgtg ccccgcggca gcacacttcc tttgctggaa
1021gggccccaga ctgatggcgt caccatcggg gacaaggggg agtgtgtgat cacgcccagc
1081acggacgtca agtttgatcc cggactcaaa agcaagaaca agctcaacta cttgaggaat
1141tactggcttc tgataggaca ccatgaaacc ccactgtctg cgtctaccaa gatgctggag
1201agatttccca acaatctacc caaacatcgg gaaaatgtga ttcctgctga ttcagagaaa
1261aagagctttg aggaagttat caacctggtt gaccagactt cagaaaacgc acctaccacc
1321gtgtctcggg atgtggagga gaagcccgcc catgcccctg cccggcccga ggcccccgtg
1381gactccatgc ttaaggacat ggctaccatc atcctgagca ccttcctgct gattggctgg
1441gtggccttca tcatcaccta tcccctgagc atgcatcagc agcagcagct ccagcaccag
1501cagttccaga aggaactgga gaagatccag ctcctgcagc agcagcagca gcagctgccc
1561ttccacccac ctggagacac ggctcaggac ggcgagctcc tggacacgtc tggcccgtac
1621tcagagagct cgggcaccag cagccccagc acgtccccca gggcctccaa ccactcgctc
1681tgctccggca gctctgcctc caaggctggc agcagcccct ccctggaaca agacgatgga
1741gatgaggaaa ccagcgtggt gatagttggg aaaatttcct tctgtcccaa ggatgtcctg
1801ggccatggag ctgagggcac aattgtgtac cggggcatgt ttgacaaccg cgacgtggcc
1861gtgaagagga tcctccccga gtgttttagc ttcgcagacc gtgaggtcca gctgttgcga
1921gaatcggatg agcacccgaa cgtgatccgc tacttctgca cggagaagga ccggcaattc
1981cagtacattg ccatcgagct gtgtgcagcc accctgcaag agtatgtgga gcagaaggac
2041tttgcgcatc tcggcctgga gcccatcacc ttgctgcagc agaccacctc gggcctggcc
2101cacctccact ccctcaacat cgttcacaga gacctaaagc cacacaacat cctcatatcc
2161atgcccaatg cacacggcaa gatcaaggcc atgatctccg actttggcct ctgcaagaag
2221gtgggcagtg gggcaaacca agttttcagc cgcccgattt ggggggccct ggcacagaag
2281gttggatcgt cccagagatg ctgagcgaag actgtaagga gaaccctacg tacacggtgg
2341acatcttttt tgcaggttgc gtcttttact acgtaatctc tgagggcagc cacctttttg
2401gcaagtccct gcagcggcag gccaacatcc tcctgggtgc ctgcagcctt gactgcttgc
2461acccagagaa gcacgaagac gtcattgcac gtgaattgat agagaagatg attgcgatgg
2521atcctcagaa acgccccTCA GCGAAGCACG TGCTCAAACA CCCGTTCTTC TGGAGCCTAG
2581AGAAGCAGCT CCAGTTCTTC CAGGACGTGA GCGACAGAAT AGAAAAGGAA TCCCTGGATG
2641GCCCGATCGT GAAGCAGTTA GAGAGAGGCG GGAGAGCCGT GGTGAAGATG GACTGGCGGG
2701AGAACATCAC TGTCCCCCTC CAGACAGACC TGCGTAAATT CAGGACCTAT AAAGGTGGTT
2761CTGTCAGAGA TCTCCTCCGA GCCATGAGAA ATAAGAAGCA CCACTACCGG GAGCTGCCTG
2821CAGAGGTGCG GGAGACGCTG GGGTCCCTCC CCGACGACTT CGTGTGCTAC TTCACGTCTC
2881GCTTCCCCCA CCTCCTCGCA CACACCTACC GGGCCATGGA GCTGTGCAGC CACGAGAGAC
2941TCTTCCAGCC CTACTACTTC CACGAGCCCC CAGAGCCCCA GCCCCCAGTG ACTCCAGACG
3001CCCTCTTGCC AACTTTCTTG TACaaagtgg ttggtaagcc tatccctaac cctctcctcg
3061gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
3121acctctggat ta

Download FASTA (ORF) (Full)