Construct: ORF ccsbBroad304_14643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019026.1_s304c1, BRDN0000459825
- Derived from:
- ccsbBroadEn_14643
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLT1 (2321)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14643
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | NM_002019.4 | 100% | 100% | |
| 2 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | XM_017020485.1 | 97.1% | 97.1% | 2591_2592ins114 |
| 3 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | NM_001160030.2 | 54.2% | 53.2% | (many diffs) |
| 4 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | XM_011535014.1 | 53.2% | 52.7% | (many diffs) |
| 5 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | NM_001159920.2 | 50.7% | 49% | (many diffs) |
| 6 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | NM_001160031.1 | 40% | 38.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4083
- ORF length:
- 4014
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcagctac tgggacaccg gggtcctgct gtgcgcgctg ctcagctgtc 121 tgcttctcac aggatctagt tcaggttcaa aattaaaaga tcctgaactg agtttaaaag 181 gcacccagca catcatgcaa gcaggccaga cactgcatct ccaatgcagg ggggaagcag 241 cccataaatg gtctttgcct gaaatggtga gtaaggaaag cgaaaggctg agcataacta 301 aatctgcctg tggaagaaat ggcaaacaat tctgcagtac tttaaccttg aacacagctc 361 aagcaaacca cactggcttc tacagctgca aatatctagc tgtacctact tcaaagaaga 421 aggaaacaga atctgcaatc tatatattta ttagtgatac aggtagacct ttcgtagaga 481 tgtacagtga aatccccgaa attatacaca tgactgaagg aagggagctc gtcattccct 541 gccgggttac gtcacctaac atcactgtta ctttaaaaaa gtttccactt gacactttga 601 tccctgatgg aaaacgcata atctgggaca gtagaaaggg cttcatcata tcaaatgcaa 661 cgtacaaaga aatagggctt ctgacctgtg aagcaacagt caatgggcat ttgtataaga 721 caaactatct cacacatcga caaaccaata caatcataga tgtccaaata agcacaccac 781 gcccagtcaa attacttaga ggccatactc ttgtcctcaa ttgtactgct accactccct 841 tgaacacgag agttcaaatg acctggagtt accctgatga aaaaaataag agagcttccg 901 taaggcgacg aattgaccaa agcaattccc atgccaacat attctacagt gttcttacta 961 ttgacaaaat gcagaacaaa gacaaaggac tttatacttg tcgtgtaagg agtggaccat 1021 cattcaaatc tgttaacacc tcagtgcata tatatgataa agcattcatc actgtgaaac 1081 atcgaaaaca gcaggtgctt gaaaccgtag ctggcaagcg gtcttaccgg ctctctatga 1141 aagtgaaggc atttccctcg ccggaagttg tatggttaaa agatgggtta cctgcgactg 1201 agaaatctgc tcgctatttg actcgtggct actcgttaat tatcaaggac gtaactgaag 1261 aggatgcagg gaattataca atcttgctga gcataaaaca gtcaaatgtg tttaaaaacc 1321 tcactgccac tctaattgtc aatgtgaaac cccagattta cgaaaaggcc gtgtcatcgt 1381 ttccagaccc ggctctctac ccactgggca gcagacaaat cctgacttgt accgcatatg 1441 gtatccctca acctacaatc aagtggttct ggcacccctg taaccataat cattccgaag 1501 caaggtgtga cttttgttcc aataatgaag agtcctttat cctggatgct gacagcaaca 1561 tgggaaacag aattgagagc atcactcagc gcatggcaat aatagaagga aagaataaga 1621 tggctagcac cttggttgtg gctgactcta gaatttctgg aatctacatt tgcatagctt 1681 ccaataaagt tgggactgtg ggaagaaaca taagctttta tatcacagat gtgccaaatg 1741 ggtttcatgt taacttggaa aaaatgccga cggaaggaga ggacctgaaa ctgtcttgca 1801 cagttaacaa gttcttatac agagacgtta cttggatttt actgcggaca gttaataaca 1861 gaacaatgca ctacagtatt agcaagcaaa aaatggccat cactaaggag cactccatca 1921 ctcttaatct taccatcatg aatgtttccc tgcaagattc aggcacctat gcctgcagag 1981 ccaggaatgt atacacaggg gaagaaatcc tccagaagaa agaaattaca atcagagatc 2041 aggaagcacc atacctcctg cgaaacctca gtgatcacac agtggccatc agcagttcca 2101 ccactttaga ctgtcatgct aatggtgtcc ccgagcctca gatcacttgg tttaaaaaca 2161 accacaaaat acaacaagag cctggaatta ttttaggacc aggaagcagc acgctgttta 2221 ttgaaagagt cacagaagag gatgaaggtg tctatcactg caaagccacc aaccagaagg 2281 gctctgtgga aagttcagca tacctcactg ttcaaggaac ctcggacaag tctaatctgg 2341 agctgatcac tctaacatgc acctgtgtgg ctgcgactct cttctggctc ctattaaccc 2401 tctttatccg aaaaatgaaa aggtcttctt ctgaaataaa gactgactac ctatcaatta 2461 taatggaccc agatgaagtt cctttggatg agcagtgtga gcggctccct tatgatgcca 2521 gcaagtggga gtttgcccgg gagagactta aactgggcaa atcacttgga agaggggctt 2581 ttggaaaagt ggttcaagca tcagcatttg gcattaagaa atcacctacg tgccggactg 2641 tggctgtgaa aatgctgaaa gagggggcca cggccagcga gtacaaagct ctgatgactg 2701 agctaaaaat cttgacccac attggccacc atctgaacgt ggttaacctg ctgggagcct 2761 gcaccaagca aggagggcct ctgatggtga ttgttgaata ctgcaaatat ggaaatctct 2821 ccaactacct caagagcaaa cgtgacttat tttttctcaa caaggatgca gcactacaca 2881 tggagcctaa gaaagaaaaa atggagccag gcctggaaca aggcaagaaa ccaagactag 2941 atagcgtcac cagcagcgaa agctttgcga gctccggctt tcaggaagat aaaagtctga 3001 gtgatgttga ggaagaggag gattctgacg gtttctacaa ggagcccatc actatggaag 3061 atctgatttc ttacagtttt caagtggcca gaggcatgga gttcctgtct tccagaaagt 3121 gcattcatcg ggacctggca gcgagaaaca ttcttttatc tgagaacaac gtggtgaaga 3181 tttgtgattt tggccttgcc cgggatattt ataagaaccc cgattatgtg agaaaaggag 3241 atactcgact tcctctgaaa tggatggctc ctgaatctat ctttgacaaa atctacagca 3301 ccaagagcga cgtgtggtct tacggagtat tgctgtggga aatcttctcc ttaggtgggt 3361 ctccataccc aggagtacaa atggatgagg acttttgcag tcgcctgagg gaaggcatga 3421 ggatgagagc tcctgagtac tctactcctg aaatctatca gatcatgctg gactgctggc 3481 acagagaccc aaaagaaagg ccaagatttg cagaacttgt ggaaaaacta ggtgatttgc 3541 ttcaagcaaa tgtacaacag gatggtaaag actacatccc aatcaatgcc atactgacag 3601 gaaatagtgg gtttacatac tcaactcctg ccttctctga ggacttcttc aaggaaagta 3661 tttcagctcc gaagtttaat tcaggaagct ctgatgatgt cagatacgta aatgctttca 3721 agttcatgag cctggaaaga atcaaaacct ttgaagaact tttaccgaat gccacctcca 3781 tgtttgatga ctaccagggc gacagcagca ctctgttggc ctctcccatg ctgaagcgct 3841 tcacctggac tgacagcaaa cccaaggcct cgctcaagat tgacttgaga gtaaccagta 3901 aaagtaagga gtcggggctg tctgatgtca gcaggcccag tttctgccat tccagctgtg 3961 ggcacgtcag cgaaggcaag cgcaggttca cctacgacca cgctgagctg gaaaggaaaa 4021 tcgcgtgctg ctccccgccc ccagactaca actcggtggt cctgtactcc accccaccca 4081 tcttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 4141 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 4201 tctggatta