Transcript: Human NM_002019.4

Homo sapiens fms related tyrosine kinase 1 (FLT1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
FLT1 (2321)
Length:
7123
CDS:
286..4302

Additional Resources:

NCBI RefSeq record:
NM_002019.4
NBCI Gene record:
FLT1 (2321)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147168 AGGTTGAGGGATACCATATG pXPR_003 CGG 1369 34% 10 1.3238 FLT1 FLT1 75559
2 BRDN0001145335 ACAGCCACAGTCCGGCACGT pXPR_003 AGG 2562 64% 18 0.4261 FLT1 FLT1 75556
3 BRDN0001145564 TGGCCACTGTGTGATCACTG pXPR_003 AGG 2003 50% 14 0.4207 FLT1 FLT1 75557
4 BRDN0001146782 CTTACCATATATATGCACTG pXPR_003 AGG 976 24% 7 0.3433 FLT1 FLT1 75558
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002019.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000230834 ACTCGTGGCTACTCGTTAATT pLKO_005 1438 CDS 100% 15.000 21.000 N FLT1 n/a
2 TRCN0000000633 CGCCGGAAGTTGTATGGTTAA pLKO.1 1376 CDS 100% 10.800 15.120 N FLT1 n/a
3 TRCN0000199542 CCATTCTCATTCGTGGCATGT pLKO.1 5056 3UTR 100% 4.050 5.670 N FLT1 n/a
4 TRCN0000230836 TGCCAGTATTATGCATATATA pLKO_005 4367 3UTR 100% 15.000 12.000 N FLT1 n/a
5 TRCN0000194670 CGTAGAGATGTACAGTGAAAT pLKO.1 690 CDS 100% 13.200 10.560 N FLT1 n/a
6 TRCN0000218557 AGACTGACTACCTATCAATTA pLKO_005 2657 CDS 100% 13.200 9.240 N FLT1 n/a
7 TRCN0000196726 GACTTGAGAGTAACCAGTAAA pLKO.1 4099 CDS 100% 13.200 9.240 N FLT1 n/a
8 TRCN0000199625 GAGCATTAGCTGGCGCATATT pLKO.1 5522 3UTR 100% 13.200 9.240 N FLT1 n/a
9 TRCN0000230833 GCCGGAAGTTGTATGGTTAAA pLKO_005 1377 CDS 100% 13.200 9.240 N FLT1 n/a
10 TRCN0000230835 TCCATACCCAGGAGTACAAAT pLKO_005 3579 CDS 100% 13.200 9.240 N FLT1 n/a
11 TRCN0000000631 CCCGTCTCTATACCAACCAAA pLKO.1 5395 3UTR 100% 4.950 3.465 N FLT1 n/a
12 TRCN0000082566 CCTCTGATGGTGATTGTTGAA pLKO.1 2995 CDS 100% 4.950 3.465 N FLT1P1 n/a
13 TRCN0000082567 CGTAAATGCTTTCAAGTTCAT pLKO.1 3924 CDS 100% 4.950 3.465 N FLT1P1 n/a
14 TRCN0000000635 CTTGACACTTTGATCCCTGAT pLKO.1 805 CDS 100% 4.050 2.835 N FLT1 n/a
15 TRCN0000000634 GAGAGACTTAAACTGGGCAAA pLKO.1 2758 CDS 100% 4.050 2.835 N FLT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002019.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14643 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_14643 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000472926 TTACTCCGCGAACAAACAATAACA pLX_317 9.6% 100% 100% V5 n/a
4 ccsbBroadEn_00580 pDONR223 100% 50.7% 49% None (many diffs) n/a
5 ccsbBroad304_00580 pLX_304 0% 50.7% 49% V5 (many diffs) n/a
6 TRCN0000479303 CACAGAAACCATGCTGTTGTAAAG pLX_317 19.1% 50.7% 49% V5 (many diffs) n/a
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