Construct: ORF ccsbBroad304_14665
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016685.2_s304c1, BRDN0000459647
- Derived from:
- ccsbBroadEn_14665
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HK1 (3098)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14665
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3098 | HK1 | hexokinase 1 | NM_000188.2 | 99.9% | 100% | 78C>G;1443G>A |
| 2 | human | 3098 | HK1 | hexokinase 1 | NM_033496.2 | 98.3% | 96% | (many diffs) |
| 3 | human | 3098 | HK1 | hexokinase 1 | NM_001322364.1 | 98.1% | 96.7% | (many diffs) |
| 4 | human | 3098 | HK1 | hexokinase 1 | NM_001358263.1 | 98.1% | 96.7% | (many diffs) |
| 5 | human | 3098 | HK1 | hexokinase 1 | NM_033497.2 | 98.1% | 96.7% | (many diffs) |
| 6 | human | 3098 | HK1 | hexokinase 1 | NM_033498.2 | 98.1% | 96.7% | (many diffs) |
| 7 | human | 3098 | HK1 | hexokinase 1 | XM_024447969.1 | 98.1% | 96.7% | (many diffs) |
| 8 | human | 3098 | HK1 | hexokinase 1 | NM_033500.2 | 97.3% | 97.2% | (many diffs) |
| 9 | human | 3098 | HK1 | hexokinase 1 | XM_011539732.1 | 97.3% | 97.2% | (many diffs) |
| 10 | human | 3098 | HK1 | hexokinase 1 | NM_001322366.1 | 96.9% | 96.9% | 0_1ins84;1359G>A |
| 11 | human | 3098 | HK1 | hexokinase 1 | XM_005269737.1 | 96.9% | 96.9% | 0_1ins84;1359G>A |
| 12 | human | 3098 | HK1 | hexokinase 1 | NM_001322367.1 | 96.4% | 96.5% | 78C>G;494_495ins96;1347G>A |
| 13 | human | 3098 | HK1 | hexokinase 1 | NM_001322365.1 | 95.1% | 94.4% | (many diffs) |
| 14 | mouse | 15275 | Hk1 | hexokinase 1 | NM_001146100.1 | 86.7% | 92.7% | (many diffs) |
| 15 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513246.3 | 85.5% | 89.2% | (many diffs) |
| 16 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513247.1 | 85.2% | 90.6% | (many diffs) |
| 17 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513243.3 | 84.7% | 89.4% | (many diffs) |
| 18 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513244.3 | 84.7% | 89.4% | (many diffs) |
| 19 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513245.3 | 84.7% | 89.4% | (many diffs) |
| 20 | mouse | 15275 | Hk1 | hexokinase 1 | NM_010438.3 | 82.7% | 87.3% | (many diffs) |
| 21 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513242.1 | 81.2% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2817
- ORF length:
- 2751
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat cgccgcgcag ctcctggcct attacttcac ggagctgaag gatgaccagg 121 tcaaaaagat tgacaagtat ctgtatgcca tgcggctctc cgatgaaact ctcatagata 181 tcatgactcg cttcaggaag gagatgaaga atggcctctc ccgggatttt aatccaacag 241 ccacagtcaa gatgttgcca acattcgtaa ggtccattcc tgatggctct gaaaagggag 301 atttcattgc cctggatctt ggtgggtctt cctttcgaat tctgcgggtg caagtgaatc 361 atgagaaaaa ccagaatgtt cacatggagt ccgaggttta tgacacccca gagaacatcg 421 tgcacggcag tggaagccag ctttttgatc atgttgctga gtgcctggga gatttcatgg 481 agaaaaggaa gatcaaggac aagaagttac ctgtgggatt cacgttttct tttccttgcc 541 aacaatccaa aatagatgag gccatcctga tcacctggac aaagcgattt aaagcgagcg 601 gagtggaagg agcagatgtg gtcaaactgc ttaacaaagc catcaaaaag cgaggggact 661 atgatgccaa catcgtagct gtggtgaatg acacagtggg caccatgatg acctgtggct 721 atgacgacca gcactgtgaa gtcggcctga tcatcggcac tggcaccaat gcttgctaca 781 tggaggaact gaggcacatt gatctggtgg aaggagacga ggggaggatg tgtatcaata 841 cagaatgggg agcctttgga gacgatggat cattagaaga catccggaca gagtttgaca 901 gggagataga ccggggatcc ctcaaccctg gaaaacagct gtttgagaag atggtcagtg 961 gcatgtactt gggagagctg gttcgactga tcctagtcaa gatggccaag gagggcctct 1021 tatttgaagg gcggatcacc ccggagctgc tcacccgagg gaagtttaac accagtgatg 1081 tgtcagccat cgaaaagaat aaggaaggcc tccacaatgc caaagaaatc ctgacccgcc 1141 tgggagtgga gccgtccgat gatgactgtg tctcagtcca gcacgtttgc accattgtct 1201 catttcgctc agccaacttg gtggctgcca cactgggcgc catcttgaac cgcctgcgtg 1261 ataacaaggg cacacccagg ctgcggacca cggttggtgt cgacggatct ctttacaaga 1321 cgcacccaca gtattcccgg cgtttccaca agactctaag gcgcttggtg ccagactccg 1381 atgtgcgctt cctcctctcg gagagtggca gcggcaaggg ggctgccatg gtgacggcgg 1441 tggcctaccg cttggccgag cagcaccggc agatagagga gaccctggct catttccacc 1501 tcaccaaaga catgctgctg gaggtgaaga agaggatgcg ggccgagatg gagctggggc 1561 tgaggaagca gacgcacaac aatgccgtgg ttaagatgct gccctccttc gtccggagaa 1621 ctcccgacgg gaccgagaat ggtgacttct tggccctgga tcttggagga accaatttcc 1681 gtgtgctgct ggtgaaaatc cgtagtggga aaaagagaac ggtggaaatg cacaacaaga 1741 tctacgccat tcctattgaa atcatgcagg gcactgggga agagctgttt gatcacattg 1801 tctcctgcat ctctgacttc ttggactaca tggggatcaa aggccccagg atgcctctgg 1861 gcttcacgtt ctcatttccc tgccagcaga cgagtctgga cgcgggaatc ttgatcacgt 1921 ggacaaaggg ttttaaggca acagactgcg tgggccacga tgtagtcacc ttactaaggg 1981 atgcgataaa aaggagagag gaatttgacc tggacgtggt ggctgtggtc aacgacacag 2041 tgggcaccat gatgacctgt gcttatgagg agcccacctg tgaggttgga ctcattgttg 2101 ggaccggcag caatgcctgc tacatggagg agatgaagaa cgtggagatg gtggaggggg 2161 accaggggca gatgtgcatc aacatggagt ggggggcctt tggggacaac gggtgtctgg 2221 atgatatcag gacacactac gacagactgg tggacgaata ttccctaaat gctgggaaac 2281 aaaggtatga gaagatgatc agtggtatgt acctgggtga aatcgtccgc aacatcttaa 2341 tcgacttcac caagaaggga ttcctcttcc gagggcagat ctctgagacg ctgaagaccc 2401 ggggcatctt tgagaccaag tttctctctc agatcgagag tgaccgatta gcactgctcc 2461 aggtccgggc tatcctccag cagctaggtc tgaatagcac ctgcgatgac agtatcctcg 2521 tcaagacagt gtgcggggtg gtgtccagga gggccgcaca gctgtgtggc gcaggcatgg 2581 ctgcggttgt ggataagatc cgcgagaaca gaggactgga ccgtctgaat gtgactgtgg 2641 gagtggacgg gacactctac aagcttcatc cacacttctc cagaatcatg caccagacgg 2701 tgaaggaact gtcaccaaaa tgtaacgtgt ccttcctcct gtctgaggat ggcagcggca 2761 agggggccgc cctcatcacg gccgtgggcg tgcggttacg cacagaggca agcagctacc 2821 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2881 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 2941 tta