Construct: ORF ccsbBroad304_14676

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018995.1_s304c1, BRDN0000459797
Derived from:
ccsbBroadEn_14676
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ITK (3702)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14676
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3702 ITK IL2 inducible T cell kinase NM_005546.4 100% 100%
2 human 3702 ITK IL2 inducible T cell kinase XM_017009443.1 79.8% 79.8% 0_1ins375
3 mouse 16428 Itk IL2 inducible T cell kinase NM_010583.3 87.9% 94.1% (many diffs)
4 mouse 16428 Itk IL2 inducible T cell kinase NM_001281968.1 87.8% 94% (many diffs)
5 mouse 16428 Itk IL2 inducible T cell kinase NM_001281966.1 87.5% 93.7% (many diffs)
6 mouse 16428 Itk IL2 inducible T cell kinase NM_001281965.1 87.1% 93.2% (many diffs)
7 mouse 16428 Itk IL2 inducible T cell kinase NM_001281967.1 37.5% 38.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1929
ORF length:
1860
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaacaacttt atcctcctgg aagaacagct catcaagaaa tcccaacaaa
121agagaagaac ttctccctcg aactttaaag tccgcttctt tgtgttaacc aaagccagcc
181tggcatactt tgaagatcgt catgggaaga agcgcacgct gaaggggtcc attgagctct
241cccgaatcaa atgtgttgag attgtgaaaa gtgacatcag catcccatgc cactataaat
301acccgtttca ggtggtgcat gacaactacc tcctatatgt gtttgctcca gatcgtgaga
361gccggcagcg ctgggtgctg gcccttaaag aagaaacgag gaataataac agtttggtgc
421ctaaatatca tcctaatttc tggatggatg ggaagtggag gtgctgttct cagctggaga
481agcttgcaac aggctgtgcc caatatgatc caaccaagaa tgcttcaaag aagcctcttc
541ctcctactcc tgaagacaac aggcgaccac tttgggaacc tgaagaaact gtggtcattg
601ccttatatga ctaccaaacc aatgatcctc aggaactcgc actgcggcgc aacgaagagt
661actgcctgct ggacagttct gagattcact ggtggagagt ccaggacagg aatgggcatg
721aaggatatgt accaagcagt tatctggtgg aaaaatctcc aaataatctg gaaacctatg
781agtggtacaa taagagtatc agccgagaca aagctgaaaa acttcttttg gacacaggca
841aagaaggagc cttcatggta agggattcca ggactgcagg aacatacacc gtgtctgttt
901tcaccaaggc tgttgtaagt gagaacaatc cctgtataaa gcattatcac atcaaggaaa
961caaatgacaa tcctaagcga tactatgtgg ctgaaaagta tgtgttcgat tccatccctc
1021ttctcatcaa ctatcaccaa cataatggag gaggcctggt gactcgactc cggtatccag
1081tttgttttgg gaggcagaaa gccccagtta cagcagggct gagatacggg aaatgggtga
1141tcgacccctc agagctcact tttgtgcaag agattggcag tgggcaattt gggttggtgc
1201atctgggcta ctggctcaac aaggacaagg tggctatcaa aaccattcgg gaaggggcta
1261tgtcagaaga ggacttcata gaggaggctg aagtaatgat gaaactctct catcccaaac
1321tggtgcagct gtatggggtg tgcctggagc aggcccccat ctgcctggtg tttgagttca
1381tggagcacgg ctgcctgtca gattatctac gcacccagcg gggacttttt gctgcagaga
1441ccctgctggg catgtgtctg gatgtgtgtg agggcatggc ctacctggaa gaggcatgtg
1501tcatccacag agacttggct gccagaaatt gtttggtggg agaaaaccaa gtcatcaagg
1561tgtctgactt tgggatgaca aggttcgttc tggatgatca gtacaccagt tccacaggca
1621ccaaattccc ggtgaagtgg gcatccccag aggttttctc tttcagtcgc tatagcagca
1681agtccgatgt gtggtcattt ggtgtgctga tgtgggaagt tttcagtgaa ggcaaaatcc
1741cgtatgaaaa ccgaagcaac tcagaggtgg tggaagacat cagtaccgga tttcggttgt
1801acaagccccg gctggcctcc acacacgtct accagattat gaatcactgc tggaaagaga
1861gaccagaaga tcggccagcc ttctccagac tgctgcgtca actggctgaa attgcagaat
1921caggactttt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
1981tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
2041tcaacctctg gatta

Download FASTA (ORF) (Full)