Construct: ORF ccsbBroad304_14676
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018995.1_s304c1, BRDN0000459797
- Derived from:
- ccsbBroadEn_14676
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITK (3702)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14676
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3702 | ITK | IL2 inducible T cell kinase | NM_005546.4 | 100% | 100% | |
| 2 | human | 3702 | ITK | IL2 inducible T cell kinase | XM_017009443.1 | 79.8% | 79.8% | 0_1ins375 |
| 3 | mouse | 16428 | Itk | IL2 inducible T cell kinase | NM_010583.3 | 87.9% | 94.1% | (many diffs) |
| 4 | mouse | 16428 | Itk | IL2 inducible T cell kinase | NM_001281968.1 | 87.8% | 94% | (many diffs) |
| 5 | mouse | 16428 | Itk | IL2 inducible T cell kinase | NM_001281966.1 | 87.5% | 93.7% | (many diffs) |
| 6 | mouse | 16428 | Itk | IL2 inducible T cell kinase | NM_001281965.1 | 87.1% | 93.2% | (many diffs) |
| 7 | mouse | 16428 | Itk | IL2 inducible T cell kinase | NM_001281967.1 | 37.5% | 38.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1929
- ORF length:
- 1860
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacaacttt atcctcctgg aagaacagct catcaagaaa tcccaacaaa 121 agagaagaac ttctccctcg aactttaaag tccgcttctt tgtgttaacc aaagccagcc 181 tggcatactt tgaagatcgt catgggaaga agcgcacgct gaaggggtcc attgagctct 241 cccgaatcaa atgtgttgag attgtgaaaa gtgacatcag catcccatgc cactataaat 301 acccgtttca ggtggtgcat gacaactacc tcctatatgt gtttgctcca gatcgtgaga 361 gccggcagcg ctgggtgctg gcccttaaag aagaaacgag gaataataac agtttggtgc 421 ctaaatatca tcctaatttc tggatggatg ggaagtggag gtgctgttct cagctggaga 481 agcttgcaac aggctgtgcc caatatgatc caaccaagaa tgcttcaaag aagcctcttc 541 ctcctactcc tgaagacaac aggcgaccac tttgggaacc tgaagaaact gtggtcattg 601 ccttatatga ctaccaaacc aatgatcctc aggaactcgc actgcggcgc aacgaagagt 661 actgcctgct ggacagttct gagattcact ggtggagagt ccaggacagg aatgggcatg 721 aaggatatgt accaagcagt tatctggtgg aaaaatctcc aaataatctg gaaacctatg 781 agtggtacaa taagagtatc agccgagaca aagctgaaaa acttcttttg gacacaggca 841 aagaaggagc cttcatggta agggattcca ggactgcagg aacatacacc gtgtctgttt 901 tcaccaaggc tgttgtaagt gagaacaatc cctgtataaa gcattatcac atcaaggaaa 961 caaatgacaa tcctaagcga tactatgtgg ctgaaaagta tgtgttcgat tccatccctc 1021 ttctcatcaa ctatcaccaa cataatggag gaggcctggt gactcgactc cggtatccag 1081 tttgttttgg gaggcagaaa gccccagtta cagcagggct gagatacggg aaatgggtga 1141 tcgacccctc agagctcact tttgtgcaag agattggcag tgggcaattt gggttggtgc 1201 atctgggcta ctggctcaac aaggacaagg tggctatcaa aaccattcgg gaaggggcta 1261 tgtcagaaga ggacttcata gaggaggctg aagtaatgat gaaactctct catcccaaac 1321 tggtgcagct gtatggggtg tgcctggagc aggcccccat ctgcctggtg tttgagttca 1381 tggagcacgg ctgcctgtca gattatctac gcacccagcg gggacttttt gctgcagaga 1441 ccctgctggg catgtgtctg gatgtgtgtg agggcatggc ctacctggaa gaggcatgtg 1501 tcatccacag agacttggct gccagaaatt gtttggtggg agaaaaccaa gtcatcaagg 1561 tgtctgactt tgggatgaca aggttcgttc tggatgatca gtacaccagt tccacaggca 1621 ccaaattccc ggtgaagtgg gcatccccag aggttttctc tttcagtcgc tatagcagca 1681 agtccgatgt gtggtcattt ggtgtgctga tgtgggaagt tttcagtgaa ggcaaaatcc 1741 cgtatgaaaa ccgaagcaac tcagaggtgg tggaagacat cagtaccgga tttcggttgt 1801 acaagccccg gctggcctcc acacacgtct accagattat gaatcactgc tggaaagaga 1861 gaccagaaga tcggccagcc ttctccagac tgctgcgtca actggctgaa attgcagaat 1921 caggactttt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1981 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 2041 tcaacctctg gatta