Construct: ORF ccsbBroad304_14683

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002005.1_s304c1, BRDN0000400215
Derived from:
ccsbBroadEn_14683
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KCNA3 (3738)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14683
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3738 KCNA3 potassium voltage-gated cha... NM_002232.5 90.8% 90.9% 1_156del;1035C>A
2 mouse 16491 Kcna3 potassium voltage-gated cha... NM_008418.2 89.9% 96.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1635
ORF length:
1569
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgac cgtggtgccc ggggaccacc tgctggagcc ggaggtggcc gatggtggag
121gggccccgcc tcaaggcggc tgtggcggcg gcggctgcga ccgctacgag ccgctgccgc
181cctcactgcc ggccgcgggc gagcaggact gctgcgggga gcgcgtggtc atcaacatct
241ccgggctgcg cttcgagacg cagctgaaga ccctttgcca gttccccgag acgctgctgg
301gcgaccccaa gcggcgcatg aggtacttcg acccgctccg caacgagtac ttcttcgacc
361gcaaccggcc cagcttcgac gccatcctct actactatca gtccgggggc cgcatccgcc
421ggccggtcaa cgtgcccatc gacattttct ccgaggagat ccgcttctac cagctgggcg
481aggaggccat ggagaagttc cgcgaggacg agggcttcct gcgggaggag gagcggccct
541tgccccgccg cgacttccag cgccaggtgt ggctgctctt cgagtacccc gagagctccg
601ggccggcccg gggcatcgcc atcgtgtccg tgctggtcat cctcatctcc attgtcatct
661tctgcctgga gacgctgccg gagttccgcg acgagaagga ctaccccgcc tcgacgtcgc
721aggactcatt cgaagcagcc ggcaacagca cgtcggggtc ccgcgcagga gcctccagct
781tctccgatcc cttcttcgtg gtggagacgc tgtgcatcat ctggttctcc ttcgaactgc
841tggtgcggtt cttcgcttgt cctagcaaag ccaccttctc gcgaaacatc atgaacctga
901tcgacattgt ggccatcatt ccttatttta tcactctggg tacagagctg gccgaacgac
961agggcaatgg acagcaggcc atgtctctgg ccatcctgag ggtcatccgc ctggtaaggg
1021tcttccgcat cttcaagctg tcgcgccact ccaaggggct gcagatcctc gggcaaacgc
1081tgaaggcgtc catgcgggag ctgggattgc tcatcttctt cctctttatt ggggtcatcc
1141ttttctccag cgcggtctac tttgccgagg cagacgaccc cacttcaggt ttcagcagca
1201tcccggatgc cttctggtgg gcagtggtaa ccatgacaac agtgggttac ggcgatatgc
1261acccagtgac catagggggc aagattgtgg gatctctctg tgccatcgcc ggtgtcttga
1321ccatcgcatt gccagttccc gtgattgttt ccaacttcaa ttacttctac caccgggaga
1381cagaagggga agagcaatcc cagtacatgc acgtgggaag ttgccagcac ctctcctctt
1441cagccgagga gctccgaaaa gcaaggagta actcgactct gagtaagtcg gagtatatgg
1501tgatcgaaga ggggggtatg aaccatagcg ctttccccca gacccctttc aaaacgggca
1561attccactgc cacctgcacc acgaacaata atcccaactc ttgtgtcaac atcaaaaaga
1621tattcaccga tgtttaccca actttcttgt acaaagtggt tggtaagcct atccctaacc
1681ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1741cgacaatcaa cctctggatt a

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