Construct: ORF ccsbBroad304_14726
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018979.1_s304c1, BRDN0000459462
- Derived from:
- ccsbBroadEn_14726
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PAK1 (5058)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14726
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | NM_002576.4 | 62.4% | 38.5% | (many diffs) |
| 2 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017846.1 | 62.4% | 38.5% | (many diffs) |
| 3 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017847.2 | 62.4% | 38.5% | (many diffs) |
| 4 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017848.1 | 62.4% | 38.5% | (many diffs) |
| 5 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017849.1 | 62.4% | 38.5% | (many diffs) |
| 6 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017850.1 | 62.4% | 38.5% | (many diffs) |
| 7 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448560.1 | 62.4% | 38.5% | (many diffs) |
| 8 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448561.1 | 62.4% | 38.5% | (many diffs) |
| 9 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448562.1 | 62.4% | 38.5% | (many diffs) |
| 10 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448563.1 | 62.4% | 38.5% | (many diffs) |
| 11 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448564.1 | 62.4% | 38.5% | (many diffs) |
| 12 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | NM_001128620.1 | 61.3% | 37.9% | (many diffs) |
| 13 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545080.3 | 61.3% | 37.9% | (many diffs) |
| 14 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545081.2 | 61.3% | 37.9% | (many diffs) |
| 15 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545082.2 | 61.3% | 37.9% | (many diffs) |
| 16 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545083.2 | 61.3% | 37.9% | (many diffs) |
| 17 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545084.3 | 61.3% | 37.9% | (many diffs) |
| 18 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448557.1 | 61.3% | 37.9% | (many diffs) |
| 19 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448558.1 | 61.3% | 37.9% | (many diffs) |
| 20 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448559.1 | 61.3% | 37.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 735
- ORF length:
- 666
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcaaataac ggcctagaca ttcaagacaa acccccagcc cctccgatga 121 gaaataccag cactatgatt ggagccggca gcaaagatgc tggaacccta aaccatggtt 181 ctaaacctct gcctccaaac ccagaggaga agaaaaagaa ggaccgattt taccgatcca 241 ttttacctgg agataaaaca aataaaaaga aagagaaaga gcggccagag atttctctcc 301 cttcagattt tgaacacaca attcatgtcg gttttgatgc tgtcacaggg gagtttacgg 361 gaatgccaga gcagtgggcc cgcttgcttc agacatcaaa tatcactaag tcggagcaga 421 agaaaaaccc gcaggctgtt ctggatgtgt tggagtttta caactcgaag aagacatcca 481 acagccagaa atacatgagc tttacagata agtcagctga ggattacaat tcttctaatg 541 ccttgaatgt gaaggctgtg tctgagactc ctgcagtgcc accagtttca gaaaatgagg 601 atgatgatga tgatgatgct accccaccac cagtgattgc tccacgccca gagcacacaa 661 aatctgtata cacacggtct gtgattgaac cacttcTTGT CAGGCGAACC CTCGCGATTC 721 AGCGAGCTCG ACAATAAAGT TTCGCTCCAT AGCTCCGGGC GACGCTTCAA GGATAATCGG 781 GCAAGTCAGA GCGATAGGGG CTCAGAATAC CAAATGTTCT CCATGTAGGG CCCTTCCAAG 841 TGGGCTAAGG TTAAAAAGGC TGCCATGAGG TCCGCAAAAT TGCAGAAGCA TCCTTCCAAA 901 TGCCATGTGC AACCAGAGAG TTTGCGATTA GCACGCTTGC GTGCCCAGGA GCCAGGGCCC 961 AGTCCTGTGA GGCATAGGAA CATTCCCCTA AACCAGTCAA GGCCTTCCCG GCACCTTCCA 1021 GTGCGGGTGG CCTCAGCAGG AAGGCAAAGG CCAATGGAAG CAGGAGCAGG CAAGGAGATG 1081 TTGAGGCCTA CAGAAAAACC AAAGTTGAGA GTAAGAGTTC AAAACCCACG GCCGGCAAGG 1141 TCAAGAATGG TGCTGTTCCC CACCAAAAAG AAGGTGGTGG CCAAGGCCAA GGCCCCAAAG 1201 CTGGGCAGGG GCCGAACACC AAGGCTGTTG CTCCTGCTAA GGCCAGCGGG TCCAAGGTGG 1261 TTCATGCACA TTTGTCCAGG AAGACAGAGG CCCCCAAGGG CCCTAGAAAG ACAGGGCTGC 1321 CCATCAAGGC CTCATCATCC AAAGTGTCCA GCCTGAGGGC TGAAGCTTAG TTGCCAACTT 1381 TTTTGTACAA AGTGGTTGGT AAGCCTATCC CCAACCCTCT CCTCGGTCTC GATTTTACGT 1441 AGTAATGAGA TAGCGCAACC CGGGCGCGTT AAGGCCACAA TCCACCTCTG GATTACAAAA 1501 TTTTGAAAGA TTGAGTGGTA TTTTTAAATA TGTTGCTCCT TTTCCGCTAT GTGGATACGC 1561 TGCTTAAAGC CTTTGATCAT ATATCTTCCA ATAAAA