Construct: ORF ccsbBroadEn_14726
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018979.1_s300c1, BRDN0000458275
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PAK1 (5058)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | NM_002576.4 | 99% | 41.1% | (many diffs) |
| 2 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017846.1 | 99% | 41.1% | (many diffs) |
| 3 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017847.2 | 99% | 41.1% | (many diffs) |
| 4 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017848.1 | 99% | 41.1% | (many diffs) |
| 5 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017849.1 | 99% | 41.1% | (many diffs) |
| 6 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017850.1 | 99% | 41.1% | (many diffs) |
| 7 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448560.1 | 99% | 41.1% | (many diffs) |
| 8 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448561.1 | 99% | 41.1% | (many diffs) |
| 9 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448562.1 | 99% | 41.1% | (many diffs) |
| 10 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448563.1 | 99% | 41.1% | (many diffs) |
| 11 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448564.1 | 99% | 41.1% | (many diffs) |
| 12 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | NM_001128620.1 | 94.7% | 40.5% | (many diffs) |
| 13 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545080.3 | 94.7% | 40.5% | (many diffs) |
| 14 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545081.2 | 94.7% | 40.5% | (many diffs) |
| 15 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545082.2 | 94.7% | 40.5% | (many diffs) |
| 16 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545083.2 | 94.7% | 40.5% | (many diffs) |
| 17 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545084.3 | 94.7% | 40.5% | (many diffs) |
| 18 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448557.1 | 94.7% | 40.5% | (many diffs) |
| 19 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448558.1 | 94.7% | 40.5% | (many diffs) |
| 20 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448559.1 | 94.7% | 40.5% | (many diffs) |
| 21 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | NM_011035.2 | 91.5% | 40.1% | (many diffs) |
| 22 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_017322028.1 | 91.5% | 40.1% | (many diffs) |
| 23 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_006507434.1 | 90.1% | 39.6% | (many diffs) |
| 24 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_006507436.3 | 90.1% | 39.6% | (many diffs) |
| 25 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_006507437.2 | 90.1% | 39.6% | (many diffs) |
| 26 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_011241694.1 | 90.1% | 39.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 132
- ORF end:
- 849
- ORF length:
- 717
- Sequence:
-
1 GGGGGGGGCG GCGCCTGAAA ATAGCCGGGC CCAATATGAT TTTATTTTGA CTGATAGTGA 61 CCTGTTCGTT GCAACAAATT GATGAGCAAT GCTTTTTTAT AATGCCAACT TTGTACAAAA 121 AAGTTGGCAC CATGTCAAAT AACGGCCTAG ACATTCAAGA CAAACCCCCA GCCCCTCCGA 181 TGAGAAATAC CAGCACTATG ATTGGAGCCG GCAGCAAAGA TGCTGGAACC CTAAACCATG 241 GTTCTAAACC TCTGCCTCCA AACCCAGAGG AGAAGAAAAA GAAGGACCGA TTTTACCGAT 301 CCATTTTACC TGGAGATAAA ACAAATAAAA AGAAAGAGAA AGAGCGGCCA GAGATTTCTC 361 TCCCTTCAGA TTTTGAACAC ACAATTCATG TCGGTTTTGA TGCTGTCACA GGGGAGTTTA 421 CGGGAATGCC AGAGCAGTGG GCCCGCTTGC TTCAGACATC AAATATCACT AAGTCGGAGC 481 AGAAGAAAAA CCCGCAGGCT GTTCTGGATG TGTTGGAGTT TTACAACTCG AAGAAGACAT 541 CCAACAGCCA GAAATACATG AGCTTTACAG ATAAGTCAGC TGAGGATTAC AATTCTTCTA 601 ATGCCTTGAA TGTGAAGGCT GTGTCTGAGA CTCCTGCAGT GCCACCAGTT TCAGAAAATG 661 AGGATGATGA TGATGATGAT GCTACCCCAC CACCAGTGAT TGCTCCACGC CCAGAGCACA 721 CAAAATCTGT ATACACACGG TCTGTGATTG AACCACTTCC TGTCACTCCA ACTCGGGAAG 781 TGGCTACCTC CTCCTTTTCA CCCTCTGAAA ATTACACCCA CTCCCCCAGA AGGTTTGGAC 841 Cggaatactg agaagcagaa gaagaagcct aaaatgtctg atgaggagat cttggagaaa 901 ttacgaagca tagtgagtgt gggcgatcct aagaagaaat atacacggtt tgagaagatt 961 ggacaaggtg cttcaggcac cgtgtacaca gcaatggatg tggccacagg acaggaggtg 1021 gccattaagc agatgaatct tcagcagcag cccaagaaag agctgattat taatgagatc 1081 ctggtcatga gggaaaacaa gAACCCAAAC ATTGTGAATT ACTTGGACAG TTACCTCGTG 1141 GGAGATGAGC TGTGGGTTGT TATGGAATAC TTGGCTGGAG GCTCCTTGAC AGATGTGGTG 1201 ACAGAAACTT GCATGGATGA AGGCCAAATT GCAGCTGTGT GCCGTGAGTG TCTGCAGGCT 1261 CTGGAGTTCT TGCATTCGAA CCAGGTCATT CACAGAGACA TCAAGAGTGA CAATATTCTG 1321 TTGGGAATGG ATGGCTCTGT CAAGCTAACT GACTTTGGAT TCTGTGCACA GATAACCCCA 1381 GAGCAGAGCA AACGGAGCAC CATGGTAGGA ACCCCATACT GGATGGCACC AGAGGTTGTG 1441 ACACGAAAGG CCTATGGGCC CAAGGTTGAC ATCTGGTCCC TGGGCATCAT GGCCATCGAA 1501 ATGATTGAAG GGGAGCCTCC ATACCTCAAT GAAAACCCTC TGAGAGCCTT GTACCTCATT 1561 GCCACCAATG GGACCCCAGA ACTTCAGAAC CCAGAGAAGC TGTCAGCTAT CTTCCGGGAC 1621 TTTCTGAACC GCTGTCTCGA GATGGATGTG GAGAAGAGAG GTTCAGCTAA AGAGCTGCTA 1681 CAGCATCAAT TCCTGAAGAT TGCCAAGCCC CTCTCCAGCC TCACTCCACT GATTGCTGCA 1741 GCTAAGGAGG CAACAAAGAA CAATCACTTG CCAACTTTCT TGTACAAAGT TGGCATTATA 1801 AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC 1861 ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATACATGGTT CATAGCTGTT 1921 TCCC