Construct: ORF ccsbBroad304_14738

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012652.2_s304c1, BRDN0000459511
Derived from:
ccsbBroadEn_14738
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDK2 (5164)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14738
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5164 PDK2 pyruvate dehydrogenase kina... NM_002611.5 100% 100%
2 human 5164 PDK2 pyruvate dehydrogenase kina... NM_001199898.1 84.2% 84.2% 0_1ins192
3 human 5164 PDK2 pyruvate dehydrogenase kina... NM_001199899.1 84.2% 84.2% 0_1ins192
4 human 5164 PDK2 pyruvate dehydrogenase kina... NM_001199900.1 46% 40.5% (many diffs)
5 mouse 18604 Pdk2 pyruvate dehydrogenase kina... NM_133667.2 91.5% 97.5% (many diffs)
6 mouse 18604 Pdk2 pyruvate dehydrogenase kina... XM_006532457.3 77% 82% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1287
ORF length:
1221
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg ctgggtgtgg gcgctgctga agaatgcgtc cctggcaggg gcgcccaagt
121acatagagca cttcagcaag ttctccccgt ccccgctgtc catgaagcag tttctggact
181tcggatccag caatgcctgt gagaaaacct ccttcacctt cctcaggcag gagctgcctg
241tgcgcctggc caacatcatg aaagagatca acctgcttcc cgaccgagtg ctgagcacac
301cctccgtgca gctggtgcag agctggtatg tccagagcct cctggacatc atggagttcc
361tggacaagga tcccgaggac catcgcaccc tgagccagtt cactgacgcc ctggtcacca
421tccggaaccg gcacaacgac gtggtgccca ccatggcaca aggcgtgctt gagtacaagg
481acacctacgg cgatgacccc gtctccaacc agaacatcca gtacttcctg gaccgcttct
541acctcagccg catctccatc cgcatgctca tcaaccagca caccctcatc tttgatggca
601gcaccaaccc agcccatccc aaacacatcg gcagcatcga ccccaactgc aacgtctctg
661aggtggtcaa agatgcctac gacatggcta agctcctgtg tgacaagtat tacatggcct
721cacctgacct ggagatccag gagatcaatg cagccaactc caaacagccg attcacatgg
781tctacgtccc ctcccacctc taccacatgc tctttgagct cttcaagaat gccatgaggg
841cgactgtgga aagccatgag tccagcctca ttctcccacc catcaaggtc atggtggcct
901tgggtgagga agatctgtcc atcaagatga gtgaccgagg tgggggtgtt cccttgagga
961agattgagcg actcttcagc tacatgtact ccacagcacc caccccccag cctggcaccg
1021ggggaacgcc gctggctggc tttggttatg ggctccccat ttcccgcctc tacgccaagt
1081acttccaggg agacctgcag ctcttctcca tggaaggctt tgggaccgat gctgtcatct
1141atctcaaggc cctgtccacg gactcggtgg agcgcctgcc tgtctacaac aagtcagcct
1201ggcgccacta ccagaccatc caggaggccg gcgactggtg tgtgcccagc acggagccca
1261agaacacgtc cacgtaccgc gtcagctacc caactttctt gtacaaagtg gttggtaagc
1321ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
1381ggcgcgttaa gtcgacaatc aacctctgga tta

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