Transcript: Mouse NM_133667.2

Mus musculus pyruvate dehydrogenase kinase, isoenzyme 2 (Pdk2), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Pdk2 (18604)
Length:
2279
CDS:
116..1339

Additional Resources:

NCBI RefSeq record:
NM_133667.2
NBCI Gene record:
Pdk2 (18604)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145761 GGTGCTGCCATCAAAGATGA pXPR_003 GGG 523 43% 5 0.501 Pdk2 PDK2 75685
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133667.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000023841 CGAGAAGACGTCATTCACTTT pLKO.1 250 CDS 100% 4.950 6.930 N Pdk2 n/a
2 TRCN0000321933 CGAGAAGACGTCATTCACTTT pLKO_005 250 CDS 100% 4.950 6.930 N Pdk2 n/a
3 TRCN0000023839 CCCAAGAACACATCGACGTAT pLKO.1 1307 CDS 100% 4.950 3.960 N Pdk2 n/a
4 TRCN0000321934 CCCAAGAACACATCGACGTAT pLKO_005 1307 CDS 100% 4.950 3.960 N Pdk2 n/a
5 TRCN0000197089 GCTCCTGTGTGACAAGTATTA pLKO.1 742 CDS 100% 13.200 9.240 N PDK2 n/a
6 TRCN0000321935 CAGGGACCTTCCCTATCTATC pLKO_005 1395 3UTR 100% 10.800 7.560 N Pdk2 n/a
7 TRCN0000321936 CATCTCCATCCGAATGCTAAT pLKO_005 601 CDS 100% 10.800 7.560 N Pdk2 n/a
8 TRCN0000023842 CCTGTGTGACAAGTATTACAT pLKO.1 745 CDS 100% 5.625 3.938 N Pdk2 n/a
9 TRCN0000322005 CCTGTGTGACAAGTATTACAT pLKO_005 745 CDS 100% 5.625 3.938 N Pdk2 n/a
10 TRCN0000195527 CCAACCAGAACATCCAGTACT pLKO.1 555 CDS 100% 4.950 3.465 N PDK2 n/a
11 TRCN0000023840 CTGGAGTACAAGGACACCTAT pLKO.1 518 CDS 100% 4.950 3.465 N Pdk2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133667.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01166 pDONR223 100% 91.5% 97.5% None (many diffs) n/a
2 ccsbBroad304_01166 pLX_304 0% 91.5% 97.5% V5 (many diffs) n/a
3 TRCN0000480761 CTTTTTCCCATACTAGCCCAATTG pLX_317 34.6% 91.5% 97.5% V5 (many diffs) n/a
4 ccsbBroadEn_14738 pDONR223 0% 91.5% 97.5% None (many diffs) n/a
5 ccsbBroad304_14738 pLX_304 0% 91.5% 97.5% V5 (many diffs) n/a
6 TRCN0000488642 TTTTAGCACGTTTTCCGACTGCTG pLX_317 28.4% 91.5% 97.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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