Construct: ORF ccsbBroad304_14787

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017459.2_s304c1, BRDN0000459584
Derived from:
ccsbBroadEn_14787
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKAR2B (5577)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14787
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5577 PRKAR2B protein kinase cAMP-depende... NM_002736.3 99.8% 99.7% 96G>T;154G>T
2 human 5577 PRKAR2B protein kinase cAMP-depende... XM_011516398.1 93.1% 93% 96G>T;154G>T;395_396ins84
3 human 5577 PRKAR2B protein kinase cAMP-depende... XM_024446836.1 61.2% 61.2% 0_1ins486
4 mouse 19088 Prkar2b protein kinase, cAMP depend... NM_011158.3 91% 96.8% (many diffs)
5 mouse 19088 Prkar2b protein kinase, cAMP depend... XM_006515014.3 91% 96.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1320
ORF length:
1254
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag catcgagatc ccggcgggac tgacggagct gctgcagggc ttcacggtgg
121aggtgctgag gcaccagccc gcggacctgc tggagttcgc tctgcagcac ttcacccgcc
181tgcagcagga gaacgagcgc aaaggcaccg cgcgcttctg ccatgagggc aggacctggg
241gggacctggg cgccgctgcc gggggcggca cccccagcaa gggggtcaac ttcgccgagg
301agcccatgca gtccgactcc gaggacgggg aggaggagga ggcggcgccc gcggacgcag
361gggcgttcaa tgctccagta ataaaccgat tcacaaggcg tgcctcagta tgtgcagaag
421cttataatcc tgatgaagaa gaagatgatg cagagtccag gattatacat ccaaaaactg
481atgatcaaag aaataggttg caagaggctt gcaaagacat cctgctgttt aagaatctgg
541atccggagca gatgtctcaa gtattagatg ccatgtttga aaaattggtc aaagatgggg
601agcatgtaat tgatcaaggt gacgatggtg acaactttta tgtaattgat agaggcacat
661ttgatattta tgtgaaatgt gatggtgttg gaagatgtgt tggtaactat gataatcgtg
721ggagtttcgg cgaactggcc ttaatgtaca atacacccag agcagctaca atcactgcta
781cctctcctgg tgctctgtgg ggtttggaca gggtaacctt caggagaata attgtgaaaa
841acaatgccaa aaagagaaaa atgtatgaaa gctttattga gtcactgcca ttccttaaat
901ctttggagtt ttctgaacgc ctgaaagtag tagatgtgat aggcaccaaa gtatacaacg
961atggagaaca aatcattgct cagggagatt cggctgattc ttttttcatt gtagaatctg
1021gagaagtgaa aattactatg aaaagaaagg gtaaatcaga agtggaagag aatggtgcag
1081tagaaatcgc tcgatgctcg cggggacagt actttggaga gcttgccctg gtaactaaca
1141aacctcgagc agcttctgcc cacgccattg ggactgtcaa atgtttagca atggatgtgc
1201aagcatttga aaggcttctg ggaccttgca tggaaattat gaaaaggaac atcgctacct
1261atgaagaaca gttagttgcc ctgtttggaa cgaacatgga tattgttgaa cccactgcat
1321gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1381attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1441ggatta

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