Transcript: Mouse XM_006515014.3

PREDICTED: Mus musculus protein kinase, cAMP dependent regulatory, type II beta (Prkar2b), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Prkar2b (19088)
Length:
1792
CDS:
263..1513

Additional Resources:

NCBI RefSeq record:
XM_006515014.3
NBCI Gene record:
Prkar2b (19088)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006515014.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360699 CGTTCAACGCTCCAGTTATAA pLKO_005 555 CDS 100% 15.000 21.000 N Prkar2b n/a
2 TRCN0000221727 CGCCACCTATGAAGAACAATT pLKO.1 1444 CDS 100% 13.200 18.480 N Prkar2b n/a
3 TRCN0000221730 CCTTCAGGAGAATAATTGTAA pLKO.1 1008 CDS 100% 5.625 7.875 N Prkar2b n/a
4 TRCN0000221731 CGCTCCAGTTATAAACCGGTT pLKO.1 562 CDS 100% 2.160 3.024 N Prkar2b n/a
5 TRCN0000221729 CGATGCAGAGTCCAGGATAAT pLKO.1 637 CDS 100% 13.200 9.240 N Prkar2b n/a
6 TRCN0000037818 GCAAAGACATCCTGCTGTTTA pLKO.1 702 CDS 100% 13.200 9.240 N PRKAR2B n/a
7 TRCN0000360627 TTGGAACAAACATGGATATTG pLKO_005 1476 CDS 100% 13.200 9.240 N Prkar2b n/a
8 TRCN0000221728 CGTCATTGACAGAGGAACATT pLKO.1 832 CDS 100% 5.625 3.938 N Prkar2b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006515014.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06773 pDONR223 100% 91% 96.8% None (many diffs) n/a
2 ccsbBroad304_06773 pLX_304 0% 91% 96.8% V5 (many diffs) n/a
3 TRCN0000480583 GCTTCGTTGCTTAAGTGCGTCTTG pLX_317 27.5% 91% 96.8% V5 (many diffs) n/a
4 ccsbBroadEn_14787 pDONR223 0% 91% 96.8% None (many diffs) n/a
5 ccsbBroad304_14787 pLX_304 0% 91% 96.8% V5 (many diffs) n/a
6 TRCN0000470400 CAGTGATTTGAACGGACTTCAATC pLX_317 32.1% 91% 96.8% V5 (many diffs) n/a
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