Transcript: Human NM_002736.3

Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
PRKAR2B (5577)
Length:
3689
CDS:
204..1460

Additional Resources:

NCBI RefSeq record:
NM_002736.3
NBCI Gene record:
PRKAR2B (5577)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147707 TTGGTAACTATGATAATCGT pXPR_003 GGG 651 52% 6 0.725 PRKAR2B PRKAR2B 76152
2 BRDN0001147301 GCGGACGCAGGGGCGTTCAA pXPR_003 TGG 302 24% 1 0.07 PRKAR2B PRKAR2B 76150
3 BRDN0001147315 TGAAAAATTGGTCAAAGATG pXPR_003 GGG 529 42% 5 -0.5220 PRKAR2B PRKAR2B 76151
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002736.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000353640 AGAGGGCACTTGGCAATTAAA pLKO_005 1843 3UTR 100% 15.000 21.000 N PRKAR2B n/a
2 TRCN0000195284 CGTTCAATGCTCCAGTAATAA pLKO.1 502 CDS 100% 15.000 21.000 N PRKAR2B n/a
3 TRCN0000380090 GGCGTTCAATGCTCCAGTAAT pLKO_005 500 CDS 100% 13.200 18.480 N PRKAR2B n/a
4 TRCN0000382429 TAATAGTCAATAGGCTTTAAC pLKO_005 1613 3UTR 100% 13.200 18.480 N PRKAR2B n/a
5 TRCN0000196680 GCTCCAGTAATAAACCGATTC pLKO.1 510 CDS 100% 6.000 8.400 N PRKAR2B n/a
6 TRCN0000196892 GTATGTGCAGAAGCTTATAAT pLKO.1 546 CDS 100% 15.000 10.500 N PRKAR2B n/a
7 TRCN0000353586 GTATGTGCAGAAGCTTATAAT pLKO_005 546 CDS 100% 15.000 10.500 N PRKAR2B n/a
8 TRCN0000330006 ATGCAGAGTCCAGGATTATAC pLKO_005 586 CDS 100% 13.200 9.240 N PRKAR2B n/a
9 TRCN0000330007 GAGCAGATGTCTCAAGTATTA pLKO_005 684 CDS 100% 13.200 9.240 N PRKAR2B n/a
10 TRCN0000194726 GATGTTATCATAGGCTATATG pLKO.1 1773 3UTR 100% 13.200 9.240 N PRKAR2B n/a
11 TRCN0000037818 GCAAAGACATCCTGCTGTTTA pLKO.1 649 CDS 100% 13.200 9.240 N PRKAR2B n/a
12 TRCN0000037814 CAAGTATTAGATGCCATGTTT pLKO.1 696 CDS 100% 5.625 3.938 N PRKAR2B n/a
13 TRCN0000037815 CCTGAAAGTAGTAGATGTGAT pLKO.1 1058 CDS 100% 4.950 3.465 N PRKAR2B n/a
14 TRCN0000194673 CGAACATGGATATTGTTGAAC pLKO.1 1429 CDS 100% 4.950 3.465 N PRKAR2B n/a
15 TRCN0000330005 CGAACATGGATATTGTTGAAC pLKO_005 1429 CDS 100% 4.950 3.465 N PRKAR2B n/a
16 TRCN0000037816 GACTGTCAAATGTTTAGCAAT pLKO.1 1310 CDS 100% 4.950 3.465 N PRKAR2B n/a
17 TRCN0000037817 GTCCAGGATTATACATCCAAA pLKO.1 593 CDS 100% 4.950 3.465 N PRKAR2B n/a
18 TRCN0000221730 CCTTCAGGAGAATAATTGTAA pLKO.1 955 CDS 100% 5.625 3.938 N Prkar2b n/a
19 TRCN0000093079 GATGAAGAAGAAGATGATGAT pLKO.1 570 CDS 100% 4.950 2.475 Y Gm5518 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002736.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06773 pDONR223 100% 99.8% 99.7% None 96G>T;154G>T n/a
2 ccsbBroad304_06773 pLX_304 0% 99.8% 99.7% V5 96G>T;154G>T n/a
3 TRCN0000480583 GCTTCGTTGCTTAAGTGCGTCTTG pLX_317 27.5% 99.8% 99.7% V5 96G>T;154G>T n/a
4 ccsbBroadEn_14787 pDONR223 0% 99.8% 99.7% None 96G>T;154G>T n/a
5 ccsbBroad304_14787 pLX_304 0% 99.8% 99.7% V5 96G>T;154G>T n/a
6 TRCN0000470400 CAGTGATTTGAACGGACTTCAATC pLX_317 32.1% 99.8% 99.7% V5 96G>T;154G>T n/a
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