Construct: ORF ccsbBroad304_14799
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018934.1_s304c1, BRDN0000459418
- Derived from:
- ccsbBroadEn_14799
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK1 (5594)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14799
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5594 | MAPK1 | mitogen-activated protein k... | NM_002745.4 | 99.5% | 98.8% | 3_5delGGC;463C>N;508C>N |
| 2 | human | 5594 | MAPK1 | mitogen-activated protein k... | NM_138957.3 | 99.5% | 98.8% | 3_5delGGC;463C>N;508C>N |
| 3 | mouse | 26413 | Mapk1 | mitogen-activated protein k... | NM_001038663.1 | 92.3% | 98.3% | (many diffs) |
| 4 | mouse | 26413 | Mapk1 | mitogen-activated protein k... | NM_011949.3 | 92.3% | 98.3% | (many diffs) |
| 5 | mouse | 26413 | Mapk1 | mitogen-activated protein k... | XM_006522147.3 | 92.3% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1146
- ORF length:
- 1077
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcggcg gcggcgggcg cgggcccgga gatggtccgc gggcaggtgt 121 tcgacgtggg gccgcgctac accaacctct cgtacatcgg cgagggcgcc tacggcatgg 181 tgtgctctgc ttatgataat gtcaacaaag ttcgagtagc tatcaagaaa atcagcccct 241 ttgagcacca gacctactgc cagagaaccc tgagggagat aaaaatctta ctgcgcttca 301 gacatgagaa catcattgga atcaatgaca ttattcgagc accaaccatc gagcaaatga 361 aagatgtata tatagtacag gacctcatgg aaacagatct ttacaagctc ttgaagacac 421 aacacctcag caatgaccat atctgctatt ttctctacca gatcctcaga gggttaaaat 481 atatccattc agctaacgtt ctgcaccgtg acctcaagcc ttccaacntg ctgctcaaca 541 ccacctgtga tctcaagatc tgtgactttg gcntggcccg tgttgcagat ccagaccatg 601 atcacacagg gttcctGACA GAATATGTGG CCACACGTTG GTACAGGGCT CCAGAAATTA 661 TGTTGAATTC CAAGGGCTAC ACCAAGTCCA TTGATATTTG GTCTGTAGGC TGCATTCTGG 721 CAGAAATGCT TTCTAACAGG CCCATCTTTC CAGGGAAGCA TTATCTTGAC CAGCTGAACC 781 ACATTTTGGG TATTCTTGGA TCCCCATCAC AAGAAGACCT GAATTGTATA ATAAATTTAA 841 AAGCTAGGAA CTATTTGCTT TCTCTTCCAC ACAAAAATAA GGTGCCATGG AACAGGCTGT 901 TCCCAAATGC TGACTCCAAA GCTCTGGACT TATTGGACAA AATGTTGACA TTCAACCCAC 961 ACAAGAGGAT TGAAGTAGAA CAGGCTCTGG CCCACCCATA TCTGGAGCAG TATTACGACC 1021 CGAGTGACGA GCCCATCGCC GAAGCACCAT TCAAGTTCGA CATGGAATTG GATGACTTGC 1081 CTAAGGAAAA GCTCAAAGAA CTAATTTTTG AAGAGACTGC TAGATTCCAG CCAGGATACA 1141 GATCTTTGCC AACTTTCTTG TACaaagtgg ttggtaagcc tatccctaac cctctcctcg 1201 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1261 acctctggat ta