Construct: ORF ccsbBroad304_14821
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015497.2_s304c1, BRDN0000459568
- Derived from:
- ccsbBroadEn_14821
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TWF1 (5756)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14821
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5756 | TWF1 | twinfilin actin binding pro... | NM_002822.5 | 71.9% | 72% | 1_294del;495C>T |
| 2 | human | 5756 | TWF1 | twinfilin actin binding pro... | NM_001242397.2 | 70.4% | 70.5% | 1_294del;483_503del;516C>T |
| 3 | human | 5756 | TWF1 | twinfilin actin binding pro... | NR_073473.2 | 24.8% | 1_421del;622C>T;1178_3042del | |
| 4 | human | 5756 | TWF1 | twinfilin actin binding pro... | NR_073472.2 | 22.3% | 1_756del;957C>T;1513_3377del | |
| 5 | mouse | 19230 | Twf1 | twinfilin actin binding pro... | XM_006520638.1 | 69.1% | 74% | (many diffs) |
| 6 | mouse | 19230 | Twf1 | twinfilin actin binding pro... | NM_008971.4 | 64% | 68.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 822
- ORF length:
- 756
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt gtatgcagca acaagagcaa ctctgaagaa ggaatttgga ggtggccaca 121 ttaaagatga agtatttgga acagtaaagg aagatgtatc attacatgga tataaaaaat 181 acttgctgtc acaatcttcc cctgccccac tgactgcagc tgaggaagaa ctacgacaga 241 ttaaaatcaa tgaggtacag actgatgtgg gtgtggacac taagcatcaa acactacaag 301 gagtagcatt tcccatttct cgagaagcct ttcaggcttt ggaaaaattg aataatagac 361 agctcaacta tgtgcagttg gaaatagata taaaaaatga aattataatt ttggccaaca 421 caacaaatac agaactgaaa gatttgccaa agaggattcc caaggattca gctcgttacc 481 atttctttct gtataaacat tcccatgaag gagactattt agagtccata gtttttattt 541 attcaatgcc tggatacaca tgcagtataa gagagcggat gctgtattct agctgcaaga 601 gccgtctgct agaaattgta gaaagacaac tacaaatgga tgtaattaga aagatcgaga 661 tagacaatgg ggatgagttg actgcagact tcctttatga agaagtacat cccaagcagc 721 atgcacacaa gcaaagtttt gcaaaaccaa aaggtcctgc aggaaaaaga ggaattcgaa 781 gactaattag gggcccagcg gaaactgaag ctactactga ttacccaact ttcttgtaca 841 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 901 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta