Construct: ORF ccsbBroad304_14830

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002048.2_s304c1, BRDN0000459776
Derived from:
ccsbBroadEn_14830
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPS6KA1 (6195)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14830
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6195 RPS6KA1 ribosomal protein S6 kinase A1 NM_002953.4 99.9% 100% 51T>G;507T>C
2 human 6195 RPS6KA1 ribosomal protein S6 kinase A1 NM_001330441.2 96.2% 95.9% (many diffs)
3 human 6195 RPS6KA1 ribosomal protein S6 kinase A1 NM_001006665.2 95.8% 95.3% (many diffs)
4 human 6195 RPS6KA1 ribosomal protein S6 kinase A1 XM_024448871.1 87.4% 87.4% 0_1ins276;231T>C
5 mouse 20111 Rps6ka1 ribosomal protein S6 kinase... NM_009097.5 91% 98.5% (many diffs)
6 mouse 20111 Rps6ka1 ribosomal protein S6 kinase... NM_001285505.1 89.5% 97% (many diffs)
7 mouse 20111 Rps6ka1 ribosomal protein S6 kinase... XM_006538677.3 87.4% 92.9% (many diffs)
8 mouse 20111 Rps6ka1 ribosomal protein S6 kinase... NM_001285506.1 86.8% 94.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2271
ORF length:
2205
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc gctcgcccag ctcaaggagc cctggccgct catggagcta gtgccgctgg
121acccggagaa tggacagacc tcaggggaag aagctggact tcagccgtcc aaggatgagg
181gcgtcctcaa ggagatctcc atcacgcacc acgtcaaggc tggctctgag aaggctgatc
241catcccattt cgagctcctc aaggttctgg gccagggatc ctttggcaaa gtcttcctgg
301tgcggaaagt cacccggcct gacagtgggc acctgtatgc tatgaaggtg ctgaagaagg
361caacgctgaa agtacgtgac cgcgtccgga ccaagatgga gagagacatc ctggctgatg
421taaatcaccc attcgtggtg aagctgcact atgccttcca gaccgagggc aagctctatc
481tcattctgga cttcctgcgt ggtggggacc tcttcacccg gctctcaaaa gaggtgatgt
541tcacggagga ggatgtgaag ttttacctgg ccgagctggc tctgggcctg gatcacctgc
601acagcctggg tatcatttac agagacctca agcctgagaa catccttctg gatgaggagg
661gccacatcaa actcactgac tttggcctga gcaaagaggc cattgaccac gagaagaagg
721cctattcttt ctgcgggaca gtggagtaca tggcccctga ggtcgtcaac cgccagggcc
781actcccatag tgcggactgg tggtcctatg gggtgttgat gtttgagatg ctgacgggct
841ccctgccctt ccaggggaag gaccggaagg agaccatgac actgattctg aaggcgaagc
901taggcatgcc ccagtttctg agcactgaag cccagagcct cttgcgggcc ctgttcaagc
961ggaatcctgc caaccggctc ggctccggcc ctgatggggc agaggaaatc aagcggcatg
1021tcttctactc caccattgac tggaataagc tataccgtcg tgagatcaag ccacccttca
1081agccagcagt ggctcagcct gatgacacct tctactttga caccgagttc acgtcccgca
1141cacccaagga ttccccaggc atccccccca gcgctggggc ccatcagctg ttccggggct
1201tcagcttcgt ggccaccggc ctgatggaag acgacggcaa gcctcgtgcc ccgcaggcac
1261ccctgcactc ggtggtacag caactccatg ggaagaacct ggtttttagt gacggctacg
1321tggtaaagga gacaattggt gtgggctcct actctgagtg caagcgctgt gtccacaagg
1381ccaccaacat ggagtatgct gtcaaggtca ttgataagag caagcgggat ccttcagaag
1441agattgagat tcttctgcgg tatggccagc accccaacat catcactctg aaagatgtgt
1501atgatgatgg caaacacgtg tacctggtga cagagctgat gcggggtggg gagctgctgg
1561acaagatcct gcggcagaag ttcttctcag agcgggaggc cagctttgtc ctgcacacca
1621ttggcaaaac tgtggagtat ctgcactcac agggggttgt gcacagggac ctgaagccca
1681gcaacatcct gtatgtggac gagtccggga atcccgagtg cctgcgcatc tgtgactttg
1741gttttgccaa acagctgcgg gctgagaatg ggctcctcat gacaccttgc tacacagcca
1801actttgtggc gcctgaggtg ctgaagcgcc agggctacga tgaaggctgc gacatctgga
1861gcctgggcat tctgctgtac accatgctgg caggatatac tccatttgcc aacggtccca
1921gtgacacacc agaggaaatc ctaacccgga tcggcagtgg gaagtttacc ctcagtgggg
1981gaaattggaa cacagtttca gagacagcca aggacctggt gtccaagatg ctacacgtgg
2041atccccacca gcgcctcaca gctaagcagg ttctgcagca tccatgggtc acccagaaag
2101acaagcttcc ccaaagccag ctgtcccacc aggacctaca gcttgtgaag ggagccatgg
2161ctgccacgta ctccgcactc aacagctcca agcccacccc ccagctgaag cccatcgagt
2221catccatcct ggcccagcgg cgagtgagga agttgccatc caccaccctg tgcccaactt
2281tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
2341agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

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