Construct: ORF ccsbBroad304_14832
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018973.1_s304c1, BRDN0000459457
- Derived from:
- ccsbBroadEn_14832
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RPS6KA3 (6197)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14832
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | NM_004586.3 | 99.5% | 30.5% | (many diffs) |
| 2 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_005274573.2 | 99.4% | 30.5% | (many diffs) |
| 3 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545556.1 | 98.6% | 30.3% | (many diffs) |
| 4 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545555.1 | 97.6% | 30.2% | (many diffs) |
| 5 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545563.3 | 95.8% | 26.7% | (many diffs) |
| 6 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029713.1 | 95.8% | 26.7% | (many diffs) |
| 7 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029714.2 | 95.8% | 26.7% | (many diffs) |
| 8 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029715.2 | 95.8% | 26.7% | (many diffs) |
| 9 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029716.1 | 95.8% | 26.7% | (many diffs) |
| 10 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029717.2 | 95.8% | 26.7% | (many diffs) |
| 11 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_006724507.3 | 95.6% | 26.7% | (many diffs) |
| 12 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029719.2 | 95.6% | 26.7% | (many diffs) |
| 13 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545557.2 | 95% | 26.5% | (many diffs) |
| 14 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545558.2 | 95% | 26.5% | (many diffs) |
| 15 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545560.1 | 95% | 26.5% | (many diffs) |
| 16 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545561.2 | 95% | 26.5% | (many diffs) |
| 17 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029718.2 | 94.3% | 24.8% | (many diffs) |
| 18 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_005274577.3 | 94.2% | 24.9% | (many diffs) |
| 19 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545562.2 | 93.6% | 24.6% | (many diffs) |
| 20 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | NM_148945.2 | 94.1% | 30.4% | (many diffs) |
| 21 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | XM_017318353.1 | 91.2% | 27.2% | (many diffs) |
| 22 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | NM_001346675.1 | 90.4% | 26.6% | (many diffs) |
| 23 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | XM_006528687.1 | 90.4% | 26.6% | (many diffs) |
| 24 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | XM_017318354.1 | 90.4% | 26.6% | (many diffs) |
| 25 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | XM_017318355.1 | 90.4% | 26.6% | (many diffs) |
| 26 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | XM_006528688.2 | 89.1% | 24.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaagg 61 ttggcatgcc gctggcgcag ctggcggacc cgtggcagaa gatggctgtg gagagcccgt 121 ccgacagcgc tgagaatgga cagcaaatta tggatgaacc tatgggagag gaggagatta 181 acccacaaac tgaagaagtc agtatcaaag aaattgcaat cacacatcat gtaaaggaag 241 gacatgaaaa ggcagatcct tcccagtttg aacttttaaa agtattaggg cagggatcat 301 ttggaaaggt tttcttagtt aaaaaaatct caggctctga tgctaggcag ctttatgcca 361 tgaaggtatt gaagaaggcc acactgaaag ttcgagaccg agttcggaca aaaatggaac 421 gtgatatctt ggtagaggtt aatcatcctt ttattgtcaa gttgcattat gcttttcaaa 481 ctgaagggaa gttgtatctt attttggatt ttctcagggg aggagatttg tttacacgct 541 tatccaaaga ggtgatgttc acagaagaag atgtcaaatt ctacttggct gaacttgcac 601 ttgctttaga ccatctacat agcctgggaa taatttatag agacttaaaa ccagaaaata 661 tacttcttga tgaagaaagt cacatcaagt taacagattt cggcctaagt aaagagtcta 721 ttgaccatga aaagaagcat attctttttg tggaactgtg gagtatatgg ctccagaagt 781 agttaatcgt cgaggtcata ctcagagtgc tgactggtgg tcttttggtg tgttaatgtt 841 tgaaatgctt actggtacac tccctttcca aggaaaagat cgaaaagaaa caatgactat 901 gattcttaaa gccaaacttg gaatgccaca gtttttgagt cctgaagcgc agagtctttt 961 acgaatgctt ttcaagcgaa atcctgcaaa cagattaggt gcaggaccag atggagttga 1021 agaaattaaa agacattcat ttttctcaac gatagactgg aataaactgt atagaagaga 1081 aattcatccg ccatttaaac ctgcaacggg caggcctgaa gatacattct attttgatcc 1141 tgagtttact gcaaaaactc ccaaagattc acctggcatt ccacctagtg ctaatgcaca 1201 tcagcttttt cgggggttta gttttgttgc tattacctca gatgatgaaa gccaagctat 1261 gcagacagtt ggtgtacatt caattgttca gcagttacac aggaacagta ttcagtttac 1321 tgatggatat gaagtaaaag aagatattgg agttggctcc tactctgttt gcaagagatg 1381 tatacataaa gctacaaaca tggagtttgc agtgaagatt attgataaaa gcaagagaga 1441 cccaacagaa gaaattgaaa ttcttcttcg ttatggacag catccaaaca tatcacctct 1501 aaaggaatgt atatgatgat ggaaagtatg tgtatgtagt aacagaactt atgaaaggag 1561 tgaatggctg gataaaattt tagacaaaat ttttctctga acgagaggcc agtgctgtcc 1621 tgttcactat aactaaaacc gttgaatatc ttcacgcaca aggggtggtt catagagact 1681 tgaaacctag caacattctt tatgtggatg aatctggtaa tccggaatct attcgaattt 1741 gtgattttgg ctttgcaaaa cagctgagag cggaaaatgg tcttctcatg actccttgtt 1801 acactgcaaa ttttgttgca ccagaggttt taaaaagaca aggctatgat gctgcttgtg 1861 atatatggag tcttggtgtc ctactctata caatgcttac cggttacact ccatttgcaa 1921 atggtcctga tgatacacca gaggaaatat tggcacgaat aggtagcgga aaattctcac 1981 tcagtggtgg ttactggaat tctgtttcag acacagcaaa ggacctggtg tcaaagatgc 2041 ttcatgtaga ccctcatcag agactgactg ctgctcttgt gctcagacat ccttggatcg 2101 tccactggga ccaactgcca caataccaac taaacagaca ggatgcacca catctagtaa 2161 agggtgccat ggcagctaca tattctgctt tgaaccgtaa tcagtcacca gttttggaac 2221 cagtaggccg ctctactctt gctcagcgga gaggtattaa aaaaatcacc tcaacagccc 2281 tgttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 2341 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 2401 tctggatta