Construct: ORF ccsbBroad304_14852
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018899.1_s304c1, BRDN0000459383
- Derived from:
- ccsbBroadEn_14852
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CDKL5 (6792)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14852
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6792 | CDKL5 | cyclin dependent kinase like 5 | NM_001323289.2 | 85.1% | 23.4% | (many diffs) |
| 2 | human | 6792 | CDKL5 | cyclin dependent kinase like 5 | NM_001037343.1 | 79.3% | 21.8% | (many diffs) |
| 3 | human | 6792 | CDKL5 | cyclin dependent kinase like 5 | NM_003159.2 | 79.3% | 21.8% | (many diffs) |
| 4 | mouse | 382253 | Cdkl5 | cyclin-dependent kinase-like 5 | NM_001024624.2 | 78% | 23.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 747
- ORF length:
- 678
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagattcct aacattggta atgtgatgaa taaatttgag atccttgggg 121 ttgtaggtga aggagcctat ggagttgtac ttaaatgcag acacaaggaa acacatgaaa 181 ttgtggcgat caagaaattc aaggacagtg aagaaaatga agaagtcaaa gaaacgactt 241 tacgagagct taaaatgctt cggactctca agcaggaaaa cattgtggag ttgaaggaag 301 catttcgtcg gaggggaaag ttgtacttgg tgtttgagta tgttgaaaaa aatatgctcg 361 aattgctgga agaaatgcca aatggagttc cacctgagaa agtaaaaagc tacatctatc 421 agctaatcaa ggctattcac tggtgccata agaatgatat tgtccatcga gatataaaac 481 cagaaaatct cttaatcagc cacaatgatg tcctaaaact gtgtgacttt ggttttgctc 541 gtaatctgtc agaaggcaat aatgctaatt acacagagta cgttgccacc agatggtatc 601 ggtccccaga actcttactt ggcgctccct atggaaagtc cgtggacatg tggtcggtgg 661 gctgtattct tggggagctt agcgatggac agcctttatt tcctggagaa agtgaaattg 721 accaactttt tactattcag aagtgctagg accacttcca tctgagcaga tgagcttttc 781 tacagtaatc tcgcttcatg ggctcggttt cagctgtaca tctcagtctg gaaagagata 841 ctgatttgat agtgtctact gactatgaga ttactgagtt ggacccagct gacagatact 901 tgacagaaca gtgtttgaat caccctacat ttcaaaccca gagacttctg gatcgttctc 961 cttcaaggtc agcaaaaaga aaaccttacc atgtggaaag cagcacattg tctaatagaa 1021 accaagccgg caaaagcact gctttgcagt ctcaccacag atctaacagc aaggacatcc 1081 agaacctgag tgtaggcctg ccccgggctg acgaaggtct ccctgccaat gaaagcttcc 1141 taaatggaaa ccttgctgga gctagtctta gtccactgca caccaaaacc taccaagcaa 1201 gcagccagcc tgggtctacc agcaaagatc tcaccaacaa caacatacca caccttctta 1261 gcccaaaaga agccaagtca aaaacagagt ttgattttaa tattgaccca aagccttcag 1321 aaggcccagg gacaaagtac ctcaagtcaa acagcagatc tcagcagaac cgccactcat 1381 tcatggaaag ctctcaaagc aaagctggga cactgcagcc caatgaaaag cagagtcggc 1441 atagctatat tgacacaatt ccccagtcct ctaggagtcc ctcctacagg accaaggcca 1501 aaagccatgg ggcactgagt gactccaagt ctgtgagcaa cctttctgaa gccagggccc 1561 aaattgcgga gcccagtacc agtaggtact tcccatctag ctgcttagac ttgaattctc 1621 ccaccagccc aacccccacc agacacagtg acacgagaac tttgctcagc ccttctggaa 1681 gaaataaccg aaatgaggga acgctggact cacgtcgaac cacaaccaga cattcttaga 1741 cgatgaggaa atgaagctgc cggagcacat gaccagtagc cattccccat tcactgtctg 1801 cactcacgaa atctttttcn tatggactgg gctacaccag ccccttttct ccccagcaac 1861 gtcctcatag gcattctatg tatgtgaccc gtgacaaagt gagagccaag ggcttggatg 1921 gaagcttgag catagggcaa gggatggcag ctagagccaa cagcctgcaa ctcttgtcac 1981 cccagcctgg agaacagctc cctccagaga tgactgtggc aagatcttcg gtcaaagaga 2041 cctccagaga aggcacctct tccttccata cacgccagaa gtctgagggt ggagtgtatc 2101 atgacccaca ctctgatgat ggcacagccc ccaaagaaaa tagacaccta tacaatgatc 2161 ctgtgccaag gagagttggt agcttttaca gagtgccatc tccacgtcca gacaattctt 2221 tccatgaaaa taatgtgtca actagagttt cttctctacc atcagagagc agttctggaa 2281 ccaaccactc aaaaagacaa ccagcattcg atccatggaa aagtcctgaa aatattagtc 2341 attcagagca actcaaggaa aaagagaagc aaggattttt caggtcaatg aaaaagaaaa 2401 agaagaaatc tcaaacagta cccaattccg acagccctga tcttctgacg ttgcagaaat 2461 ccattcattc tgctagcact ccaagcagca gaccaaagga gtggcgcccc gagaagatct 2521 cagatctgca gaccttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 2581 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2641 cgacaatcaa cctctggatt a