Construct: ORF ccsbBroad304_14864

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019060.1_s304c1, BRDN0000459859
Derived from:
ccsbBroadEn_14864
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TIE1 (7075)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14864
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7075 TIE1 tyrosine kinase with immuno... NM_005424.5 99.9% 100% 2334T>C
2 human 7075 TIE1 tyrosine kinase with immuno... XM_005271163.2 96.1% 96.2% 911_912ins129;2205T>C
3 human 7075 TIE1 tyrosine kinase with immuno... NM_001253357.2 95.9% 95.8% (many diffs)
4 human 7075 TIE1 tyrosine kinase with immuno... XR_946751.2 85.2% (many diffs)
5 human 7075 TIE1 tyrosine kinase with immuno... XM_017002207.2 72.2% 70.6% (many diffs)
6 human 7075 TIE1 tyrosine kinase with immuno... XM_006710869.1 68.7% 68.8% 0_1ins1065;1269T>C
7 mouse 21846 Tie1 tyrosine kinase with immuno... NM_011587.2 87.3% 92.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3483
ORF length:
3414
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggtctggcgg gtgccccctt tcttgctccc catcctcttc ttggcttctc
121atgtgggcgc ggcggtggac ctgacgctgc tggccaacct gcggctcacg gacccccagc
181gcttcttcct gacttgcgtg tctggggagg ccggggcggg gaggggctcg gacgcctggg
241gcccgcccct gctgctggag aaggacgacc gtatcgtgcg caccccgccc gggccacccc
301tgcgcctggc gcgcaacggt tcgcaccagg tcacgcttcg cggcttctcc aagccctcgg
361acctcgtggg cgtcttctcc tgcgtgggcg gtgctggggc gcggcgcacg cgcgtcatct
421acgtgcacaa cagccctgga gcccacctgc ttccagacaa ggtcacacac actgtgaaca
481aaggtgacac cgctgtactt tctgcacgtg tgcacaagga gaagcagaca gacgtgatct
541ggaagagcaa cggatcctac ttctacaccc tggactggca tgaagcccag gatgggcggt
601tcctgctgca gctcccaaat gtgcagccac catcgagcgg catctacagt gccacttacc
661tggaagccag ccccctgggc agcgccttct ttcggctcat cgtgcggggt tgtggggctg
721ggcgctgggg gccaggctgt accaaggagt gcccaggttg cctacatgga ggtgtctgcc
781acgaccatga cggcgaatgt gtatgccccc ctggcttcac tggcacccgc tgtgaacagg
841cctgcagaga gggccgtttt gggcagagct gccaggagca gtgcccaggc atatcaggct
901gccggggcct caccttctgc ctcccagacc cctatggctg ctcttgtgga tctggctgga
961gaggaagcca gtgccaagaa gcttgtgccc ctggtcattt tggggctgat tgccgactcc
1021agtgccagtg tcagaatggt ggcacttgtg accggttcag tggttgtgtc tgcccctctg
1081ggtggcatgg agtgcactgt gagaagtcag accggatccc ccagatcctc aacatggcct
1141cagaactgga gttcaactta gagacgatgc cccggatcaa ctgtgcagct gcagggaacc
1201ccttccccgt gcggggcagc atagagctac gcaagccaga cggcactgtg ctcctgtcca
1261ccaaggccat tgtggagcca gagaagacca cagctgagtt cgaggtgccc cgcttggttc
1321ttgcggacag tgggttctgg gagtgccgtg tgtccacatc tggcggccaa gacagccggc
1381gcttcaaggt caatgtgaaa gtgccccccg tgcccctggc tgcacctcgg ctcctgacca
1441agcagagccg ccagcttgtg gtctccccgc tggtctcgtt ctctggggat ggacccatct
1501ccactgtccg cctgcactac cggccccagg acagtaccat ggactggtcg accattgtgg
1561tggaccccag tgagaacgtg acgttaatga acctgaggcc aaagacagga tacagtgttc
1621gtgtgcagct gagccggcca ggggaaggag gagagggggc ctgggggcct cccaccctca
1681tgaccacaga ctgtcctgag cctttgttgc agccgtggtt ggagggctgg catgtggaag
1741gcactgaccg gctgcgagtg agctggtcct tgcccttggt gcccgggcca ctggtgggcg
1801acggtttcct gctgcgcctg tgggacggga cacgggggca ggagcggcgg gagaacgtct
1861catcccccca ggcccgcact gccctcctga cgggactcac gcctggcacc cactaccagc
1921tggatgtgca gctctaccac tgcaccctcc tgggcccggc ctcgccccct gcacacgtgc
1981ttctgccccc cagtgggcct ccagcccccc gacacctcca cgcccaggcc ctctcagact
2041ccgagatcca gctgacatgg aagcacccgg aggctctgcc tgggccaata tccaagtacg
2101ttgtggaggt gcaggtggct gggggtgcag gagacccact gtggatagac gtggacaggc
2161ctgaggagac aagcaccatc atccgtggcc tcaacgccag cacgcgctac ctcttccgca
2221tgcgggccag cattcagggg ctcggggact ggagcaacac agtagaagag tccaccctgg
2281gcaacgggct gcaggctgag ggcccagtcc aagagagccg ggcagctgaa gagggcctgg
2341atcagcagct gatcctggcg gtggtgggct ccgtgtctgc cacctgcctc accatcctgg
2401ccgccctttt aaccctggtg tgcatccgca gaagctgcct gcatcggaga cgcaccttca
2461cctaccagtc aggctcgggc gaggagacca tcctgcagtt cagctcaggg accttgacac
2521ttacccggcg gccaaaactg cagcccgagc ccctgagcta cccagtgcta gagtgggagg
2581acatcacctt tgaggacctc atcggggagg ggaacttcgg ccaggtcatc cgggccatga
2641tcaagaagga cgggctgaag atgaacgcag ccatcaaaat gctgaaagag tatgcctctg
2701aaaatgacca tcgtgacttt gcgggagaac tggaagttct gtgcaaattg gggcatcacc
2761ccaacatcat caacctcctg ggggcctgta agaaccgagg ttacttgtat atcgctattg
2821aatatgcccc ctacgggaac ctgctagatt ttctgcggaa aagccgggtc ctagagactg
2881acccagcttt tgctcgagag catgggacag cctctaccct tagctcccgg cagctgctgc
2941gtttcgccag tgatgcggcc aatggcatgc agtacctgag tgagaagcag ttcatccaca
3001gggacctggc tgcccggaat gtgctggtcg gagagaacct agcctccaag attgcagact
3061tcggcctttc tcggggagag gaggtttatg tgaagaagac gatggggcgt ctccctgtgc
3121gctggatggc cattgagtcc ctgaactaca gtgtctatac caccaagagt gatgtctggt
3181cctttggagt ccttctttgg gagatagtga gccttggagg tacaccctac tgtggcatga
3241cctgtgccga gctctatgaa aagctgcccc agggctaccg catggagcag cctcgaaact
3301gtgacgatga agtgtacgag ctgatgcgtc agtgctggcg ggaccgtccc tatgagcgac
3361ccccctttgc ccagattgcg ctacagctag gccgcatgct ggaagccagg aaggcctatg
3421tgaacatgtc gctgtttgag aacttcactt acgcgggcat tgatgccaca gctgaggagg
3481ccttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
3541tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
3601tctggatta

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