Construct: ORF ccsbBroad304_14864
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019060.1_s304c1, BRDN0000459859
- Derived from:
- ccsbBroadEn_14864
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIE1 (7075)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14864
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7075 | TIE1 | tyrosine kinase with immuno... | NM_005424.5 | 99.9% | 100% | 2334T>C |
| 2 | human | 7075 | TIE1 | tyrosine kinase with immuno... | XM_005271163.2 | 96.1% | 96.2% | 911_912ins129;2205T>C |
| 3 | human | 7075 | TIE1 | tyrosine kinase with immuno... | NM_001253357.2 | 95.9% | 95.8% | (many diffs) |
| 4 | human | 7075 | TIE1 | tyrosine kinase with immuno... | XR_946751.2 | 85.2% | (many diffs) | |
| 5 | human | 7075 | TIE1 | tyrosine kinase with immuno... | XM_017002207.2 | 72.2% | 70.6% | (many diffs) |
| 6 | human | 7075 | TIE1 | tyrosine kinase with immuno... | XM_006710869.1 | 68.7% | 68.8% | 0_1ins1065;1269T>C |
| 7 | mouse | 21846 | Tie1 | tyrosine kinase with immuno... | NM_011587.2 | 87.3% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3483
- ORF length:
- 3414
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtctggcgg gtgccccctt tcttgctccc catcctcttc ttggcttctc 121 atgtgggcgc ggcggtggac ctgacgctgc tggccaacct gcggctcacg gacccccagc 181 gcttcttcct gacttgcgtg tctggggagg ccggggcggg gaggggctcg gacgcctggg 241 gcccgcccct gctgctggag aaggacgacc gtatcgtgcg caccccgccc gggccacccc 301 tgcgcctggc gcgcaacggt tcgcaccagg tcacgcttcg cggcttctcc aagccctcgg 361 acctcgtggg cgtcttctcc tgcgtgggcg gtgctggggc gcggcgcacg cgcgtcatct 421 acgtgcacaa cagccctgga gcccacctgc ttccagacaa ggtcacacac actgtgaaca 481 aaggtgacac cgctgtactt tctgcacgtg tgcacaagga gaagcagaca gacgtgatct 541 ggaagagcaa cggatcctac ttctacaccc tggactggca tgaagcccag gatgggcggt 601 tcctgctgca gctcccaaat gtgcagccac catcgagcgg catctacagt gccacttacc 661 tggaagccag ccccctgggc agcgccttct ttcggctcat cgtgcggggt tgtggggctg 721 ggcgctgggg gccaggctgt accaaggagt gcccaggttg cctacatgga ggtgtctgcc 781 acgaccatga cggcgaatgt gtatgccccc ctggcttcac tggcacccgc tgtgaacagg 841 cctgcagaga gggccgtttt gggcagagct gccaggagca gtgcccaggc atatcaggct 901 gccggggcct caccttctgc ctcccagacc cctatggctg ctcttgtgga tctggctgga 961 gaggaagcca gtgccaagaa gcttgtgccc ctggtcattt tggggctgat tgccgactcc 1021 agtgccagtg tcagaatggt ggcacttgtg accggttcag tggttgtgtc tgcccctctg 1081 ggtggcatgg agtgcactgt gagaagtcag accggatccc ccagatcctc aacatggcct 1141 cagaactgga gttcaactta gagacgatgc cccggatcaa ctgtgcagct gcagggaacc 1201 ccttccccgt gcggggcagc atagagctac gcaagccaga cggcactgtg ctcctgtcca 1261 ccaaggccat tgtggagcca gagaagacca cagctgagtt cgaggtgccc cgcttggttc 1321 ttgcggacag tgggttctgg gagtgccgtg tgtccacatc tggcggccaa gacagccggc 1381 gcttcaaggt caatgtgaaa gtgccccccg tgcccctggc tgcacctcgg ctcctgacca 1441 agcagagccg ccagcttgtg gtctccccgc tggtctcgtt ctctggggat ggacccatct 1501 ccactgtccg cctgcactac cggccccagg acagtaccat ggactggtcg accattgtgg 1561 tggaccccag tgagaacgtg acgttaatga acctgaggcc aaagacagga tacagtgttc 1621 gtgtgcagct gagccggcca ggggaaggag gagagggggc ctgggggcct cccaccctca 1681 tgaccacaga ctgtcctgag cctttgttgc agccgtggtt ggagggctgg catgtggaag 1741 gcactgaccg gctgcgagtg agctggtcct tgcccttggt gcccgggcca ctggtgggcg 1801 acggtttcct gctgcgcctg tgggacggga cacgggggca ggagcggcgg gagaacgtct 1861 catcccccca ggcccgcact gccctcctga cgggactcac gcctggcacc cactaccagc 1921 tggatgtgca gctctaccac tgcaccctcc tgggcccggc ctcgccccct gcacacgtgc 1981 ttctgccccc cagtgggcct ccagcccccc gacacctcca cgcccaggcc ctctcagact 2041 ccgagatcca gctgacatgg aagcacccgg aggctctgcc tgggccaata tccaagtacg 2101 ttgtggaggt gcaggtggct gggggtgcag gagacccact gtggatagac gtggacaggc 2161 ctgaggagac aagcaccatc atccgtggcc tcaacgccag cacgcgctac ctcttccgca 2221 tgcgggccag cattcagggg ctcggggact ggagcaacac agtagaagag tccaccctgg 2281 gcaacgggct gcaggctgag ggcccagtcc aagagagccg ggcagctgaa gagggcctgg 2341 atcagcagct gatcctggcg gtggtgggct ccgtgtctgc cacctgcctc accatcctgg 2401 ccgccctttt aaccctggtg tgcatccgca gaagctgcct gcatcggaga cgcaccttca 2461 cctaccagtc aggctcgggc gaggagacca tcctgcagtt cagctcaggg accttgacac 2521 ttacccggcg gccaaaactg cagcccgagc ccctgagcta cccagtgcta gagtgggagg 2581 acatcacctt tgaggacctc atcggggagg ggaacttcgg ccaggtcatc cgggccatga 2641 tcaagaagga cgggctgaag atgaacgcag ccatcaaaat gctgaaagag tatgcctctg 2701 aaaatgacca tcgtgacttt gcgggagaac tggaagttct gtgcaaattg gggcatcacc 2761 ccaacatcat caacctcctg ggggcctgta agaaccgagg ttacttgtat atcgctattg 2821 aatatgcccc ctacgggaac ctgctagatt ttctgcggaa aagccgggtc ctagagactg 2881 acccagcttt tgctcgagag catgggacag cctctaccct tagctcccgg cagctgctgc 2941 gtttcgccag tgatgcggcc aatggcatgc agtacctgag tgagaagcag ttcatccaca 3001 gggacctggc tgcccggaat gtgctggtcg gagagaacct agcctccaag attgcagact 3061 tcggcctttc tcggggagag gaggtttatg tgaagaagac gatggggcgt ctccctgtgc 3121 gctggatggc cattgagtcc ctgaactaca gtgtctatac caccaagagt gatgtctggt 3181 cctttggagt ccttctttgg gagatagtga gccttggagg tacaccctac tgtggcatga 3241 cctgtgccga gctctatgaa aagctgcccc agggctaccg catggagcag cctcgaaact 3301 gtgacgatga agtgtacgag ctgatgcgtc agtgctggcg ggaccgtccc tatgagcgac 3361 ccccctttgc ccagattgcg ctacagctag gccgcatgct ggaagccagg aaggcctatg 3421 tgaacatgtc gctgtttgag aacttcactt acgcgggcat tgatgccaca gctgaggagg 3481 ccttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 3541 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 3601 tctggatta