Construct: ORF ccsbBroad304_14877

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018969.1_s304c1, BRDN0000459453
Derived from:
ccsbBroadEn_14877
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
VRK2 (7444)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14877
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7444 VRK2 VRK serine/threonine kinase 2 NM_001130480.2 98.6% 48.3% (many diffs)
2 human 7444 VRK2 VRK serine/threonine kinase 2 NM_001130481.2 98.6% 48.3% (many diffs)
3 human 7444 VRK2 VRK serine/threonine kinase 2 NM_001288837.1 98.6% 48.3% (many diffs)
4 human 7444 VRK2 VRK serine/threonine kinase 2 NM_006296.6 98.6% 48.3% (many diffs)
5 human 7444 VRK2 VRK serine/threonine kinase 2 XM_005264540.4 98.6% 48.3% (many diffs)
6 human 7444 VRK2 VRK serine/threonine kinase 2 XM_011533092.3 98.6% 48.3% (many diffs)
7 human 7444 VRK2 VRK serine/threonine kinase 2 NM_001130482.2 94.2% 43.8% (many diffs)
8 human 7444 VRK2 VRK serine/threonine kinase 2 XM_006712090.4 76.6% 61.8% (many diffs)
9 human 7444 VRK2 VRK serine/threonine kinase 2 XM_017004858.2 76.6% 61.8% (many diffs)
10 human 7444 VRK2 VRK serine/threonine kinase 2 NM_001130483.2 76.5% 61.9% (many diffs)
11 human 7444 VRK2 VRK serine/threonine kinase 2 NM_001288838.1 76.5% 61.9% (many diffs)
12 human 7444 VRK2 VRK serine/threonine kinase 2 XM_006712091.4 76.5% 61.9% (many diffs)
13 human 7444 VRK2 VRK serine/threonine kinase 2 NM_001288836.1 75.6% 25.1% (many diffs)
14 human 7444 VRK2 VRK serine/threonine kinase 2 NM_001288839.1 75.6% 25.1% (many diffs)
15 human 7444 VRK2 VRK serine/threonine kinase 2 XM_006712092.4 75.6% 25.1% (many diffs)
16 human 7444 VRK2 VRK serine/threonine kinase 2 XM_006712093.4 75.6% 25.1% (many diffs)
17 human 7444 VRK2 VRK serine/threonine kinase 2 XM_017004859.2 75.6% 25.1% (many diffs)
18 human 7444 VRK2 VRK serine/threonine kinase 2 XM_017004860.2 75.6% 25.1% (many diffs)
19 human 7444 VRK2 VRK serine/threonine kinase 2 XM_017004861.2 75.6% 25.1% (many diffs)
20 human 7444 VRK2 VRK serine/threonine kinase 2 XM_017004862.2 75.6% 25.1% (many diffs)
21 human 7444 VRK2 VRK serine/threonine kinase 2 XM_017004863.2 75.6% 25.1% (many diffs)
22 human 7444 VRK2 VRK serine/threonine kinase 2 XM_017004865.1 53.4% 32.2% (many diffs)
23 human 7444 VRK2 VRK serine/threonine kinase 2 XM_017004866.1 53.4% 32.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
831
ORF length:
765
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc accaaaaaga aatgaaaaat acaaacttcc tattccattt ccagaaggca
121aggttctgga tgatatggaa ggcaatcagt gggtactggg caagaagatt ggctctggag
181gatttggatt gatatattta gctttcccca caaataaacc agagaaagat gcaagacatg
241tagtaaaagt ggaatatcaa gaaaatggcc cgttattttc agaacttaaa ttttatcaga
301gagttgcaaa aaaagactgt atcaaaaagt ggatagaacg caaacaactt gattatttag
361gaattcctct gttttatgga tctggtctga ctgaattcaa gggaagaagt tacagattta
421tggtaatgga aagactagga atagatttac agaagatctc aggccagaat ggtaccttta
481aaaagtcaac tgtcctgcaa ttaggtatcc gaatgttgga tgtactggaa tatatacatg
541aaaatgaata tgttcatggt gatataaaag cagcaaatct acttttgggt tacaaaaatc
601cagaccaggt ttatcttgca gattatggac tttcctacag atattgtccc aatgggaacc
661acaaacagta tcaggaaaat cctagaaaag gccataatgg gacaatagag tttaccagct
721tggatgccca caagggagta gccttgtcca gacgaagtga cgttgagatc ctcggctact
781gcatgctggg ggtgnntggg tgtgggaaac ttcccttggg gaacagaacc tgaaggaccc
841ctgtggctgt gcagactgct aaaaacaaat tcttgttgga cgagctcccc cagtcagtgc
901ttaatnggct ccctctggaa gcagtgnctg tgaaatagcc caatttttgg tatgtgctca
961tagtttagca tatgatgaaa agccaaacta tcaagccctc aagaaaattt tgaaccctca
1021tggaatacct ttaggaccac tggacttttc cacaaaagga cagagtataa atgtccatac
1081tccaaacagt caaaaagttg attcacaaaa ggctgcaaca aagcaagtca acaaggcaca
1141caataggtta atcgaaaaaa aagtccacag tgagagaagc gctgagtcct gtgcaacatg
1201gaaagtgcag aaagaggaga aactgattgg attgatgaac aatgaagcag ctcaggaaag
1261cacaaggaga agacagaaat atcaagagtc tcaagaacct ttgaatgaag taaacagttt
1321cccacaaaaa atcagctata cacaattccc aaactcattt tatgagcctc atcaagattt
1381taccagtcca gatatattca agaagtcaag atctccatct tggtataaat acacttccac
1441agtcagcacg gggatcacag acttagaaag ttcaactgga ctttggccta caatttccca
1501gtttactctt agtgaagaga caaacgcaga tgtttattat tatcgcatca tcatacctgt
1561ccttttgatg ttagtatttc ttgctttatt ttttctcttg ccaactttct tgtacaaagt
1621ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag
1681cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)