Construct: ORF ccsbBroad304_14887

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014252.2_s304c1, BRDN0000459706
Derived from:
ccsbBroadEn_14887
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAPKAPK3 (7867)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14887
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7867 MAPKAPK3 MAPK activated protein kina... NM_001243925.2 100% 100%
2 human 7867 MAPKAPK3 MAPK activated protein kina... NM_001243926.1 100% 100%
3 human 7867 MAPKAPK3 MAPK activated protein kina... NM_004635.4 100% 100%
4 human 7867 MAPKAPK3 MAPK activated protein kina... XR_940501.2 40.5% 1_126del;629_630ins124;1149_2396del
5 mouse 102626 Mapkapk3 mitogen-activated protein k... NM_178907.3 89.6% 94.2% (many diffs)
6 mouse 102626 Mapkapk3 mitogen-activated protein k... XM_006511616.3 89.6% 94.2% (many diffs)
7 mouse 102626 Mapkapk3 mitogen-activated protein k... XM_006511617.3 76.4% 78.6% (many diffs)
8 mouse 102626 Mapkapk3 mitogen-activated protein k... XM_006511618.3 75% 79.1% (many diffs)
9 mouse 102626 Mapkapk3 mitogen-activated protein k... XM_006511619.1 65.7% 70.1% (many diffs)
10 mouse 102626 Mapkapk3 mitogen-activated protein k... NM_001316691.1 62.1% 58% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1212
ORF length:
1146
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga tggtgaaaca gcagaggagc aggggggccc tgtgcccccg ccagttgcac
121ccggcggacc cggcttgggc ggtgctccgg gggggcggcg ggagcccaag aagtacgcag
181tgaccgacga ctaccagttg tccaagcagg tgctgggcct gggtgtgaac ggcaaagtgc
241tggagtgctt ccatcggcgc actggacaga agtgtgccct gaagctcctg tatgacagcc
301ccaaggcccg gcaggaggta gaccatcact ggcaggcttc tggcggcccc catattgtct
361gcatcctgga tgtgtatgag aacatgcacc atggcaagcg ctgtctcctc atcatcatgg
421aatgcatgga aggtggtgag ttgttcagca ggattcagga gcgtggcgac caggctttca
481ctgagagaga agctgcagag ataatgcggg atattggcac tgccatccag tttctgcaca
541gccataacat tgcccaccga gatgtcaagc ctgaaaacct actctacaca tctaaggaga
601aagacgcagt gcttaagctc accgattttg gctttgctaa ggagaccacc caaaatgccc
661tgcagacacc ctgctatact ccctattatg tggcccctga ggtcctgggt ccagagaagt
721atgacaagtc atgtgacatg tggtccctgg gtgtcatcat gtacatcctc ctttgtggct
781tcccaccctt ctactccaac acgggccagg ccatctcccc ggggatgaag aggaggattc
841gcctgggcca gtacggcttc cccaatcctg agtggtcaga agtctctgag gatgccaagc
901agctgatccg cctcctgttg aagacagacc ccacagagag gctgaccatc actcagttca
961tgaaccaccc ctggatcaac caatcgatgg tagtgccaca gaccccactc cacacggccc
1021gagtgctgca ggaggacaaa gaccactggg acgaagtcaa ggaggagatg accagtgcct
1081tggccactat gcgggtagac tacgaccagg tgaagatcaa ggacctgaag acctctaaca
1141accggctcct caacaagagg agaaaaaagc aggcaggcag ctcctctgcc tcacagggct
1201gcaacaacca gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1261tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1321caatcaacct ctggatta

Download FASTA (ORF) (Full)

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