Transcript: Human NM_001243926.1

Homo sapiens MAPK activated protein kinase 3 (MAPKAPK3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-21
Taxon:
Homo sapiens (human)
Gene:
MAPKAPK3 (7867)
Length:
3047
CDS:
643..1791

Additional Resources:

NCBI RefSeq record:
NM_001243926.1
NBCI Gene record:
MAPKAPK3 (7867)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147502 ACATAATAGGGAGTATAGCA pXPR_003 GGG 609 53% 8 1.4424 MAPKAPK3 MAPKAPK3 77240
2 BRDN0001146065 GTGTATGAGAACATGCACCA pXPR_003 TGG 323 28% 5 0.554 MAPKAPK3 MAPKAPK3 77241
3 BRDN0001146642 CTTGGACAACTGGTAGTCGT pXPR_003 CGG 124 11% 4 0.2522 MAPKAPK3 MAPKAPK3 77239
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001243926.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000006156 CCTCATCATCATGGAATGCAT pLKO.1 984 CDS 100% 3.000 4.200 N MAPKAPK3 n/a
2 TRCN0000196478 GAACTTAAGGTGGGAGATATT pLKO.1 2516 3UTR 100% 13.200 10.560 N MAPKAPK3 n/a
3 TRCN0000006154 CCCTGCTATACTCCCTATTAT pLKO.1 1246 CDS 100% 15.000 10.500 N MAPKAPK3 n/a
4 TRCN0000297329 CCCTGCTATACTCCCTATTAT pLKO_005 1246 CDS 100% 15.000 10.500 N MAPKAPK3 n/a
5 TRCN0000006153 GCCTGCATAACAGACTGAAAT pLKO.1 1824 3UTR 100% 13.200 9.240 N MAPKAPK3 n/a
6 TRCN0000279762 GCCTGCATAACAGACTGAAAT pLKO_005 1824 3UTR 100% 13.200 9.240 N MAPKAPK3 n/a
7 TRCN0000195452 CATTAACCTGAGGTCTCTTTC pLKO.1 2712 3UTR 100% 10.800 7.560 N MAPKAPK3 n/a
8 TRCN0000279765 CATTAACCTGAGGTCTCTTTC pLKO_005 2712 3UTR 100% 10.800 7.560 N MAPKAPK3 n/a
9 TRCN0000199830 GCAGCACCTTTAGCTAGGTTG pLKO.1 2035 3UTR 100% 4.050 2.835 N MAPKAPK3 n/a
10 TRCN0000279834 GCAGCACCTTTAGCTAGGTTG pLKO_005 2035 3UTR 100% 4.050 2.835 N MAPKAPK3 n/a
11 TRCN0000199756 GCCCTGAAGCTCCTGTATGAC pLKO.1 853 CDS 100% 1.650 1.155 N MAPKAPK3 n/a
12 TRCN0000199453 GCCCAAGAAGTACGCAGTGAC pLKO.1 741 CDS 100% 1.350 0.945 N MAPKAPK3 n/a
13 TRCN0000279835 GCCCAAGAAGTACGCAGTGAC pLKO_005 741 CDS 100% 1.350 0.945 N MAPKAPK3 n/a
14 TRCN0000006157 GCGGGATATTGGCACTGCCAT pLKO.1 1083 CDS 100% 0.880 0.616 N MAPKAPK3 n/a
15 TRCN0000006155 GCTCCTCAACAAGAGGAGAAA pLKO.1 1722 CDS 100% 0.495 0.297 N MAPKAPK3 n/a
16 TRCN0000412509 CCACTATGCGGGTAGACTATG pLKO_005 1661 CDS 100% 10.800 15.120 N Mapkapk3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001243926.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01836 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01836 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000472636 CATGCACCTCCGACAATCCGGGCT pLX_317 17.8% 100% 100% V5 n/a
4 ccsbBroadEn_14887 pDONR223 0% 100% 100% None n/a
5 ccsbBroad304_14887 pLX_304 0% 100% 100% V5 n/a
6 TRCN0000480399 GCCCCCGCTTATCCTTTAGTCGCA pLX_317 30.3% 100% 100% V5 n/a
7 TRCN0000488944 CCACTAAATTGGAGTTATGCTGCA pLX_317 26% 100% 100% V5 (not translated due to prior stop codon) n/a
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