Construct: ORF ccsbBroad304_14910
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018877.1_s304c1, BRDN0000459361
- Derived from:
- ccsbBroadEn_14910
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RIPK1 (8737)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14910
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8737 | RIPK1 | receptor interacting serine... | NM_001354930.2 | 67.9% | 37% | (many diffs) |
| 2 | human | 8737 | RIPK1 | receptor interacting serine... | NM_003804.6 | 67.9% | 37% | (many diffs) |
| 3 | human | 8737 | RIPK1 | receptor interacting serine... | NM_001354931.2 | 61.5% | 30.3% | (many diffs) |
| 4 | human | 8737 | RIPK1 | receptor interacting serine... | XM_006715237.3 | 59.5% | 68.9% | (many diffs) |
| 5 | human | 8737 | RIPK1 | receptor interacting serine... | XM_017011405.1 | 59.5% | 68.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 909
- ORF length:
- 840
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaaccagac atgtccttga atgtcattaa gatgaaatcc agtgacttcc 121 tggagagtgc agaactggac agcggaggct tcgggaaggt gtctctgtgt ttccacagaa 181 cccagggact catgatcatg aaaacagtgt acaaggggcc caactgcatt gagcacaacg 241 aggccctctt ggaggaggcg aagatgatga acagactgag acacagccgg gtggtgaagc 301 tcctgggcgt catcatagag gaagggaagt actccctggt gatggagtac atggagaagg 361 gcaacctgat gcacgtgctg aaagccgaga tgagtactcc gctttctgta aaaggaagga 421 taattttgga aatcattgaa ggaatgtgct acttacatgg aaaaggcgtg atacacaagg 481 acctgaagcc tgaaaatatc cttgttgata atgacttcca cattaagatc gcagacctcg 541 gccttgcctc ctttaagatg tggagcaaac tgaataatga agagcacaat gagctgaggg 601 aagtggacgg caccgctaag aagaatggcg gcaccctcta ctacatggcg cccgagcacc 661 tgaatgacgt caacgcaaag cccacagaga agtcggatgt gtacagcttt gctgtagtac 721 tctgggcgat atttgcaaat aaggagccat atgaaaatgc tatctgtgag cagcagttga 781 taatgtgcat aaaatctggg aacaggccag atgtgatgac atcactgagt actgcccaga 841 gaaattatca gtctcatgaa gctctgctgg gaagcgaatc cggaagctcg gccgacattt 901 cctggcattg aagaaaaatt taggcctttt tatttaagtc aattagaaga aagtgtagaa 961 gaggacgtga agagtttaaa gaaagagtat tcaaacgaaa atgcagttgt gaagagaatg 1021 cagtctcttc aacttgattg tgtggcagta ccttcAGGCA GCTCGGTATA ATGATAACTA 1081 TAGAGATAAT TAGGTAATTC CCGTAACGTC CCAAAATAGC CTCTTCACTA CATCTGGGAT 1141 TGGCTGAGAG GTCGGGCCGG CTTCGGCCTT TGCATAAATA AAAAAATTAG TCAGCCATGG 1201 GTCGGCGAAT GAGCAGACCG GGCGGAGTTA GGGGCGACAT GGCCGGAGTT AGGGGCGGGA 1261 TAGCTAGAGC CAGACATGAT AAGATACATT GATGAGTTTG GACAACCCTC AACTAGAATG 1321 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT 1381 ATAAGTTGCA ATAAACAAGT TCCTCTCACT CTCTGATATT CATTTCTTTG CAAGTTATAA 1441 ATACTGAATA ATAAGATGAC ATGAACTACT ATACGTACTG CTAGAGATTT TCCACACTGA 1501 CTAAAAGGGT CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC 1561 TTGAAGCACT CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA 1621 GAGCTCCCAG GCTCAGATCT GGTCTAACCA GAGAGACCCG TTTATGTATC GAGATAGGCA 1681 CTTAAATACA ATATCTATGC AATGCGGCAA TTCAGTGGTT CGTCCAATCC ATGTCAGACC 1741 CGTCTGTTGC CTTCCTAATA AGGCACGATC GTACCACCTT ACTTCCACCA ATCGGCATGC 1801 ACGGTGCTTT TTCTCTCCTT GGAAGGCATG TTGCTAACTC ATCGTTACCA TGTTGCAAGA 1861 CTACAAGAGT ATTGCATAAG ACTACATTAA GCTTGCAGCT CCAGCTTTTG TTGCCTTTAG 1921 TGAGGGAAAT GCGCGACTTG TGGATTAGTT CTTCG