Construct: ORF ccsbBroad304_14974

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018890.1_s304c1, BRDN0000459374
Derived from:
ccsbBroadEn_14974
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAP3K2 (10746)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14974
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10746 MAP3K2 mitogen-activated protein k... NM_001371910.2 98.6% 34.5% (many diffs)
2 human 10746 MAP3K2 mitogen-activated protein k... NM_001371911.1 98.6% 34.5% (many diffs)
3 human 10746 MAP3K2 mitogen-activated protein k... NM_006609.5 98.6% 34.5% (many diffs)
4 mouse 26405 Map3k2 mitogen-activated protein k... NM_011946.3 90.7% 32.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
828
ORF length:
759
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggatgatcag caagctttga actcaatcat gcaagatttg gctgtccttc
121ataaggccag tcgaccagca ttatccttgc aggaaaccag aaaagcaaaa tcttcatcac
181caaaaaaaca gaatgatgtc cgagtcaaat ttgaacatag aggagaaaaa agaatccttc
241agttccccag accagttaaa ctggaagatc tgagatctaa agctaaaatt gcctttggac
301agtctatgga tctacattat accaataacg agttggtaat tccattaact actcaagatg
361acttggacaa agctgtggaa ctgctggatc gtagtattca tatgaagagc ctcaagatat
421tacttgtaat aaatggaagt acacaggcta ctaatttaga accattgcca tcactagaag
481atttggataa tacagtattt ggagcagaga ggaaaaaacg gctatctata ataggtccta
541ctagtagaga tagaagttct cctcccccag gttacattcc agatgaatta caccaggttg
601cccggaatgg gtcattcact agtatcaaca gtgaaggaga gttcattcca gagagcatgg
661accaaatgct ggatccatta tctttaagca gccctgaaaa ttctggctca gaagttgtcc
721atcacttgat agtcctttgg atggagagag ctatccaaaa tcacgaatgc ctagggctca
781gagctaccca gatatcatca gattttcaga ctatgatacc ctatctttga gaattgggaa
841agagacatat ccagaaggta tcatgtttca tatcatcatc aagagtataa tgatggtcgt
901aaaacttttc caagagctag aaggacccag gggaccagct tacggtctcc tgtgagtttc
961agtcctactg atcattcctt aagcactagt agtggaagca gtatctttac cccagagtat
1021gatgatagtc gaataagaag aaggggaagt gacatagaca atcctacttt gaccgtaatg
1081gacatcagcc cacccagccg ttcacctcga gctccgacca cttggaagat gggcaaactg
1141cttgcccaag gagcctttgg naaggtctac ctctgttatg atgtgattac aggaagagaa
1201tttgctgtaa gcaaagttca atttgacccc gatagtcntg AGACCAGCAA GGAAGTAAAT
1261GCACTTGAGT GTGAAATTCA GTTGCTGAAA AACTTGCTAC ATGAGCGAAT TGTTCAGTAT
1321TATGGCTGTT TGAGGGATCC CCAGGAAAAA ACACTTTCCA TATTTATGGA ATATATGCCA
1381GGGGGTTCAA TTAAGGACCA ATTAAAAGCA TATGGCGCTC TTACTGAGAA TGTGACTAGG
1441AAATACACCC GTCAGATTCT GGAGGGTGTC CATTATTTGC ACAGTAATAT GATTGTCCAT
1501AGAGATATCA AAGGCGCAAA TATCCTGCGA GATTCAACAG GCAACGTCAA ACTAGGAGAT
1561TTTGGGGCCA GCAAACGGCT TCAGACCATC TGTCTCTCAG GGACAGGAAT GAAGTCTGTC
1621ACGGGCACAC CATACTGGAT GAGCCCTGAA GTCATCAGTG GAGAAGGCTA TGGAAGAAAA
1681GCAGACATCT GGAGTGTTGC ATGTACTGTG GTAGAAATGC TAACTGAAAA GCCGCCTTGG
1741GCTGAATTTG AAGCAATGGC TGCCATCTTT AAAATCGCCA CTCAGCCAAC AAACCCAAAG
1801CTGCCACCTC ATGTCTCAGA CTATACTCGA GATTTCCTCA AACGGATTTT TGTAGAGGCC
1861AAACTGAGAC CTTCAGCTGA TGAACTCTTA AGGCACATGT TTGTGCATTA TCACTTGCCA
1921ACTTTCTTGT ACaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct
1981acgtagtaat gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt
2041a

Download FASTA (ORF) (Full)